Fa 23 Microbial Lab Schedule - REVISED_-359802875
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MCB 3020C: Microbial Biology Fall 2023 - REVISED
Lab Schedule – subject to change
Date
Lab Activities
Lab Manual Sections
8/23
lab safety & ubiquity of microbes & Winogradsky column
introduction
Section 2-1, 8-1, pg 1-7
8/25
ubiquity of microbes & microscope
Section 2-1, 2-2, 3-1, 3-2, pg 181-184
8/30
Hurricane Idalia
9/1
aseptic technique
Winogradsky Discussion post due
Section 1-3, pg 27, Appendix C
9/6
simple stains
Section 3-5, 3-6, pg 181-184
9/8
simple stain, negative stains, & pour plates
Section 3-5, 3-6, 3-9, pg 181-184
9/13
quadrant streak plates & spread plates
Section 1-4, Canvas Handout
9/15
Differential staining: Gram, Endospore and Acid Fast
Section 3-7, 3-8, 3-10
9/20
Differential staining: Gram, Endospore and Acid Fast
Section 3-7, 3-8, 3-10
9/22
Catch up & practice for hands on exam
Microscope worksheet Due
9/27
Lab Written Exam
9/29
Hands On Exam:
Gram Stain, Streak and Spread Plates
10/4
Quantification of bacteria: standard plate count - Meat
experiment part 1
Section 6-1, Canvas Handout
10/6
Quantification of bacteria: standard plate count analysis -
Meat experiment part 2 &
Receive Unknown 1
Quantification of Bacteria Due
Winogradsky Discussion post due
Section 6-1, Canvas Handout
Date
Lab Activities
Lab Manual Sections
10/11
Aerotolerance (deeps, shakes, thioglycollate, gas pak)
Section 2-6, 2-7, 2-8
10/13
Carbohydrate fermentation (oxidation-fermentation,
MRVP)
Meat Assignment due
Section 5-2, 5-4, pg 286
10/18
Carbohydrate fermentation (phenol red broth, KIA)
Aerotolerance Quiz
Section 5-3, 5-21, pg 286
10/20
Carbohydrate fermentation (SIM, motility, citrate utilization)
Section 5-9, 5-20, 5-28
10/25
Respiration & Metabolism (catalase, oxidase, nitrate
reduction)
Section 5-6, 5-7, 5-8
10/27
Bacterial Report Introduction
Carbohydrate Quiz
Section7-7, Canvas Handout
11/1
Selective & Differential media
(Mannitol salt, MacConkey agar, EMB)
Respiration/Metabolism Quiz
Section 4-2, 4-4, 4-5, 4-6, pg 236
11/3
Differential Media (Blood agar, Columbia CNA, DNase)
Winogradsky Discussion post due
Section 5-25, canvas handout
11/8
Unknown 2 Introduction
Receive unknown 2
11/10
NO LAB - Veteran’s Day
11/15
analysis of unknown
Selective/Differential Media Quiz
Bacterial Identification Report 1
11/17
analysis of unknown
DNA sample prep
11/22
NO LAB – Thanksgiving
11/24
NO LAB - Thanksgiving
11/29
analysis of unknown
12/1
analysis of unknown
Blast Day
12/6
analysis of unknown
Winogradsky Column Mature Assignment due
12/8
Winogradsky Columns & analysis of unknown
Bacterial Identification Report 2
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