Graded HW 5-2

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Please paraphrase how both experiments (from fig.2 and 3) are asking differing questions

The spread of the corona virus is now becoming enormous. In your point of view as a senior high school STEM student, what can you contribute or suggest to the government that will help in blessing the COVID-19 active casesin your community and flattening curve?

Give typed explanation

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B7

7 A-C

9. Please help answer this question and please show all work on how you got the answers. Thank you so much!! :)

This figure shows the frequency of the different variants of the novel coronavirus over time colored as each variant. I need help understanding what the figure tells me about the relative fitnesses of the delta variant compared to the alpha variant?

There are currently 3 types of Covid- 19 vaccinations. What is the best Covid-19 vaccination and why?

hello, any help would be greatly appreciated!

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Considering counting rules, calculate the initial titre of the sample viral stock if the following plaques were counted in a plaque assay. Show clear sample calculations. Table 1: Plaque assay counts for lambda phage stock titre enumeration of average concentration (PFU/mL) Total dilution of viral stock 1/13500000 1/135000000 1/135000000 plated Volume diluted viral stock 0.1 0.1 0.1 plated (mL) PFU replicate 1 TNTC 275 25 Calculated concentration (PFU/mL) replicate 1 PFU replicate 2 TNTC 239 23 Calculated concentration (PFU/mL) replicate 2 Average concentration (PFU/mL)

In which strategies would gene W be inserted into the vector in only one direction?

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How many fragments will be formed after digestion in sample C, if the plasmid is circular and why? How was RAD52 expression observed?

E. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host range, is temperature sensitive, and produces clear plaques (genotype h st c); another strain carries the wildtype alleles (genotype h+ st+ c+). Progeny phages are collected from the lysed cells and are plated on bacteria. The following numbers of different progeny phages are obtained: Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 h+ c st 26 h c+ st+ 30 h+ c st+ 106 h c+ st 110 h+ c+ st 5 h c st+ 6 a. Determine the order of the three genes on the phage chromosome. b. Determine the map distances between the genes. c. Determine the coefficient of coincidence and the interference

Section B: Circular DNA Plasmid map Eco01091 2674 Pfol 46 BSTAPI 179 Aatil 2617 Ndel 183 Sspl 2501 Ehel 235 Pdml 2294 146/ 236 lacz Bcgl 2215 Scal 2177 \2486 MCS 469 PUC18/19 2686 bp BsaXI 659 Sapl 683 852 AflI, Pscl 806 Gsul 1784 1626 Cfr101 1779. Eco311 1766 Eam11051 1694 1466 Гер (pMB1) BseYl 1110 \Cail 1217 Ed Acs CCT CCA GGT CGA CrC TAG AGG ATC CC GGG TAC CGA CCT CGA ATT CT MT CAT G07 CAT AGC TGT 7C CtG 3 ITA OTA CCA GTA TCI ACA ANG GC Y 3 C ATT TO Crs cOG Lacz Val Val Al Leu Ass The e et Thr Met 1. You have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp, What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions. a. Pscl & Gsul b. Scal, Pdml & BsaXI c. Scal, Sspl & Ehel 2. You have determined that the amplicon you want to clone has enzyme restriction sites for Hindll and ECORI, After investigation you have seen that…

Determine the coefficient of coincidence and the interference

Redraw Figure 10-23 to include the positions of the single and double crossovers

None

Diagram and explain how APEX probes can be used to determine that an individual is CC (homozygous) for a specific G/C SNV. Recall that the genotype of an SNV is identified from the strand shown in NCBI. What color fluorescence will be observed? Also, explain why a left apex probe cannot be used for this SNV. The SNV sequence, on the strand shown in NCBI, and a few nucleotides adjacent to the SNV are below: 5'-------TGT(G/C)CAG------3'

I need help because i don't get it. Can you help me to explain to me, please?

Ans 24-28

I know the answer is e but can you thoroughly explain the answer choices? like what does constitutive, inducible, expressed mean? and how do these conditions come about?

LO65 Compare methods that Covid tests and Covid vaccines use. What should the mRNA molecule of a regular mRNA vaccine contain to be translated accurately by our cells? a-Manking UTRS b-alternative codont c-DNA template strand d-promoter e-poly A tail f- S cap    asap please

Protein vaccines include only the parts (i.e. a protein) of a virus that best stimulate your immune system. This type of COVID-19 vaccine contains harmless S proteins. Once your immune system recognizes the S proteins, it creates antibodies and defensive white blood cells. If you later become infected with the COVID-19 virus, the antibodies will fight the virus. Assume that you work in a project for development of such vaccine against COVID-19. The vaccine contains S Protein and your job includes isolation of pure S Protein from the virus determination of charge on the protein (or pI) determination of molecular mass revealing three dimensional (3-D) structure of the protein How would you isolate (purify) the protein; suggest different techniques and explain the techniques. How would you determine the pI of the protein? Suggest techniques for determination of molecular mass. How would you analyze the structure of the protein?

Protein vaccines include only the parts (i.e. a protein) of a virus that best stimulate your immune system. This type of COVID-19 vaccine contains harmless S proteins. Once your immune system recognizes the S proteins, it creates antibodies and defensive white blood cells. If you later become infected with the COVID-19 virus, the antibodies will fight the virus. Assume that you work in a project for development of such vaccine against COVID-19. The vaccine contains S Protein and your job includes isolation of pure S Protein from the virus determination of charge on the protein (or pI) determination of molecular mass revealing three dimensional (3-D) structure of the protein How would you determine the pI of the protein?

Protein vaccines include only the parts (i.e. a protein) of a virus that best stimulate your immune system. This type of COVID-19 vaccine contains harmless S proteins. Once your immune system recognizes the S proteins, it creates antibodies and defensive white blood cells. If you later become infected with the COVID-19 virus, the antibodies will fight the virus. Assume that you work in a project for development of such vaccine against COVID-19. The vaccine contains S Protein and your job includes isolation of pure S Protein from the virus determination of charge on the protein (or pI) determination of molecular mass revealing three dimensional (3-D) structure of the protein Suggest techniques for determination of molecular mass. How would you analyze the structure of the protein?