Lab 11 blood typing

What is the analysis and conclusion? in the picture.

True or false. 1. In an immunoassay for drugs of abuse, any concentration greater than 0 is considered positive screen. 2. Traditionaly, drug screens are performed on blood samples because drug concentration in blood are much higher then in urine?

if the hplc chromatogram showed a large peak for LLL triglyceride, why would this be inappropriate for olive oil?

What other diseases/conditions can you find an increased LAP activity? Give a brief description of each disease/condition. What other diseases/condition can you find a decreased LAP activity? Give a brief description of each disease/condition. Is the LAP Cytochemical Test 100% diagnostic for any particular disorder? Explain your answer Are there any disadvantages for this test? Briefly describe these possible

Qualitative Test for Proteins

1

Complete the table:                                                                                     Test/ samples Glucose     Starch Molisch’s test   Purple ring     Fehling’s test Red precipitate Red precipitate No precipitate No precipitate Barfoed’s test   No precipitate No precipitate   Iodine test No colour   No colour Blue colour

Name two more methods, besides the Bradford Assay, for determining protein concentration.

You have obtained a protein lysate with a concentration of 1.05 mg/ml.  You wish to load 10 ug of this lysate into your gel.  What volume (in ul) should you load (that is equivalent to 10 ug)?

A solution contains alanine and serine. What are the possible dipeptide products that can form from this mixture? H H H H T HIL N- -O-H EN- H CH3 H CH₂OH serine (ser) alanine (ala) Enter each answer as two three-letter codes joined with a dash (e.g. ala-ala). Check Next (1 of 2) -O-H

You can use this as your reference: https://www.youtube.com/watch?v=2J2t5FRnMtM

What does high NaCl do to protein that is bound to a Sp column?

A1L TPN order is written for 400 mL 10% Dextrose, 300 mL 4.25% amino acids, and a total of 37 mL of additives. How much sterile water for injection should be added? a. 55 mL b. 137 mL c. 163 mL d. 263 mL

17. Which of the following scenarios DOES NOT point to the procurement of an assay?A. a certain type of grass is collected from a forest, and was analyzed for its overall mercury (Hg) content in its rootsB. a blood sample was taken from a dead dolphin for a complete genome sequencingC. human saliva is collected for the testing of covid-19 genomeD. a sample of urine was collected from a suspected drunk driver for analysis of phenolic compounds

Chemistry Question

How would one calculate the protein concentration for each sample if the original protein concentration is 2mg/mL. ?

PROCEDURES 1. Search at least five food products at home. 2. Determine the major fat component (Saturated or Unsaturated) RESULTS FOOD PRODUCTS French Fries fried using vegetable oil French Fries fried using Types of Fats Unsaturated Advantages/Disadvantages It has health benefits/It is costlier Saturated It is much cheaper/ Not healthy – fats will be deposited in veins derived from animal oil -- CONCLUSIONS

Morphine should be extracted from a urine sample using solid extraction (SPE). Suggest a suitable SPE column that can be used for the extraction of morphine. The answer must be substantiated. The structure of morphine is shown in Figure 1. НО. N-CH3 Но Figure 1: The structure of morphine. The amino group has a pkg value equal to 8.21.

1. Would you say that indicator tests are qualitative or quantitative? Explain your answer. 2. a. How does the Benedict test detect monosaccharides? (be specific!) b. Why can't the Benedict's test easily detect the sugar units of a starch? How does the Iodine test detect starch? (be specific!) b. Why can't the sugar units of a monosaccharide solution be detected with Iodine? (hint: "because it's not designed to" is not the answer I'm looking for!) 3. а. 4. a. What was different about the two protein solutions? b. How does the Biuret indicator detect proteins (and thus only one of the protein solutions)?

Highest Ką value Ka Lowest Ką value Ka Answer Bank CICH₂CH₂COOH CICH, COOH Cl₂ CHCOOH CH₂CH₂COOH 2

Complete the table below regarding the different laboratory tests done for Amino Acids and Proteins Positive Results/ Observations Positive for (provide details like functional group responsible) List Down Samples that will yield positive result* Procedure (Chemical Reagents Added) (ex: color of solution, precipitate formation, etc) Tests Biuret Test Ninhydrin Xanthoproteic Millon's Sakaguchi Hopkin's Cole Lead Acetate Br, in water Nitroprusside Reaction Pauly's Test Folin-Coicalteau's Test *Samples to consider: All the Standard Amino Acids Albumin Casein

amanda found an article that stated 5 mg/ml of nicotine (mw 162.23 g/mol) enters the blood stream after smoking a hookah pen. she wants to investigate the potential hazards of such high amount of nicotine in the blood. what molar concentration should she use to replicate this phenomena in vitro.

II. Analysis 1. Answer the following questions based on the given tables. % Fatty acid by weight Fat or oil Palmitic Stearic Oleic Linoleic Lard 24.6 15.0 50.4 10.0 Olive 14.6 75.4 10.0 Peanut 8.5 6.00 51.6 26.0 Corn 6.0 2.0 44.0 48.0 1. has the highest iodine number? 2. has the lowest melting point? 3. requires the least number of moles hydrogen for complete hydrogenation? 4. would likely be solid at room temperature? 5-6. Arrange based on melting temperature (highest to lowest). _7-8. Arrange based on tendency to solidify first as temperature is decreased (slowest to fastest). _9-10. Arrange based on degree of unsaturation (highest to lowest).

A student is testing for the presence of protein, simple sugars, starch, and lipids using the tests summarized in the table below.  Molecule Test Positive Result Negative Result Protein Biuret reagent Pink or purple Blue Simple sugars Benedict's reagent Green, Orange or Red Blue Starch Iodine Orange, Blue or Purple Yellow Lipids Brown paper Oily Stain No stain   The student tests an unknown solution using each of the tests and observes that the solution turns blue-black when he tests for protein. Which of the following conclusions can the student make? A. The solution contains carbohydrates. B. The solution contains lipids. C. No conclusion can be drawn; the student made an error. D. The solution contains starch. E. The solution contains protein.

vo(pM/min) 100 90 80 70 60 50 40 30, 20 10 0 What is V max for this enzyme ? Enzyme concentration 0.1 µM 5 10 15 20 A. 100 mm/min B. 1.0 mM/min C. 0.5 μM/min D. 50 µM/min E. 90 µM/min 25 30 35 37

Neonatal bilirubin samples are especially vulnerable to UV light degradation due to their small sample volume.   Question 9 options:   True   False