Prelab for Experiment 6 Chromatography of Kool Aid
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California State University, Los Angeles *
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1010
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Chemistry
Date
Feb 20, 2024
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2
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Jacob Irra
CHEM 1010-05
1.
What scientific concept(s) is this lab about? Identify the scientific concept(s) (principle, theory, law) of the lab and write what you know about the concept(s) from the lab manual, textbook, class notes, handouts, etc.
The scientific concept this lab will be about is Chromatography. Chromatography is one of the most common analytical techniques used in laboratories around the world because it is a versatile
technique for determining the chemical composition of a wide variety of materials and only requires small amounts of samples for analytics. In chromatography, a mixture, usually in the form of a liquid or gas, is passed through another substance, usually a solid that retains and separates the components contained in the mixture. 2.
What are the objectives for this lab? Describe the specific actions you are being asked to perform in the lab, such as measure something, analyze something, test something, etc.
-
Measure
-
Mix
-
Analyze the data
3.
Briefly describe how what you are being asked to do in the lab (the objectives) will help you learn about the lab's scientific concept(s). In other words, show the link between your
response to question #2 (what you will do in the lab) to your response to question #1 (what you are supposed to be learning about by doing the lab).
We will be using the paper chromatography to separate the color components contained in different flavors of Kool-Aid drink mix and compare the color components to samples of FD&C dyes. By doing this we will be able to understand how chromatography is used and how a liquid mixture is passed through another substance that separates the components contained in the mixture. 4.
What is your hypothesis for the lab experiment? Your hypothesis may be stated in 1-2 sentences.
Jacob Irra
CHEM 1010-05
My hypothesis is that each flavor of Kool-Aid will produce different chromatography patterns due to variations in their color components, and these patterns will differ from those of the FD&C dyes used as standards.
Pre-lab questions from Manual
1.
A food sample contains three unknown color pigments with the codes O, Y, and G. After a paper chromatography experiment separates the pigments in the food sample, O is calculated to have the highest R, value while G has the lowest R, value. a.
Which pigment has the greatest intermolecular interactions with the stationary phase? The pigment G has the greatest intermolecular interactions with the stationary phase.
b.
Which pigment has the greatest intermolecular interactions with the mobile phase? The pigment O has the greatest intermolecular interactions with the mobile phase.
2. From the experiment in Question 1, the following data were obtained: Distance traveled by spot O 5.5 cm
Rf = 5.5 cm / 6.0 cm = 0.92
Distance traveled by spot Y 4.0 cm
Rf = 4.0 cm / 6.0 cm = 0.67
Distance traveled by spot G 3.0 cm
Rf = 3.0 cm / 6.0 cm = 0.50
Distance traveled by solvent front 6.0 cm Calculate the retention factor (Rf) for each spot.
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liquid
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Ox10
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1
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M Inbox ( x
Precalc X
A HOLID X
Oluwa X
Good
ь Chemi X
A fake X
G spiral
G the sar X
X Micros X
https://papers.xtremepape.rs/CAIE/IGCSE/Chemistry%20(0620)/0620_s05_qp_6.pdf
The same mass of zinc was used each time. The volume of acid used was different each
time. Use the syringe diagrams to record the volume of hydrogen produced each time in the
table.
Table of results
volume of sulphuric
acid/cm
volume of
syringe diagram
hydrogen/cm3
10
20 30 40 50 60
70 80
10
20 30 40 50
60 70
80
15
10 20 30 40
50
60
70 80
20
10
20 30 40 50
60 70
80
25
10 20 30 40 50 60
70 80
30
10 20 30 40 50 60 70 80
10
20
30 40
50 60
70 80
35
40
10 20 30 40 50 60
70 80
ENG
23:09
O Type here to search
27°C
US
21/04/2022
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