Lab 3 MW

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Arizona State University *

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114

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Chemistry

Date

Dec 6, 2023

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pdf

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3

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Spectrophotometric Analysis of Copper Solutions Record all measured data and calculated data with the correct number of significant figures and with correct units. Experiment 1: Determining Wavelength of Maximum Absorption 1. Describe the solution concentrations and the wavelengths of maximum absorption for both the copper(II) sulfate and the cobalt(II) chloride solutions. Also record the absorbance value at the wavelength of maximum absorption. 2. (a) Compare the color of each solution to the color of the wavelength of light that is absorbed most effectively – shown by the wavelength of maximum absorption. For each solution, how do the colors relate to one another on a color wheel? Google "color wheel" if you don't happen to have one handy! The CuSO4 is blue but absorbs red more than any other color. The CoCl2 is red but absorbs green more than any other color. (b) Explain your answer to 2(a) in terms of “light absorbed” vs. “light reflected” by the solution. The CuSO4 is refracting a blue color which means that it absorbs all other colors. The CoCl2 is refracting a red color which means that it absorbs all other colors. Solution Concentration ( M ) Wavelength max (nm) Absorbance CuSO 4 .060M 700 0.63 CoCl 2 .025M 500 0.23
Experiment 2: Developing a Standard Curve from Known Concentrations 3. Show your work for calculating the concentration of CuSO 4 in first four solutions . Remember significant figures and units. 𝐶𝑢𝑆𝑂 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛 = . 060 𝑚𝑜𝑙 𝐿 15 𝑡𝑜𝑡𝑎𝑙 𝑚𝐿 ∗ 3.0𝑚𝐿 𝐶𝑢𝑆𝑂 = 0.012𝑀 𝐶𝑢𝑆𝑂 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛 = . 060 𝑚𝑜𝑙 𝐿 15 𝑡𝑜𝑡𝑎𝑙 𝑚𝐿 ∗ 6.0𝑚𝐿 𝐶𝑢𝑆𝑂 = 0.024𝑀 𝐶𝑢𝑆𝑂 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛 = . 060 𝑚𝑜𝑙 𝐿 15 𝑡𝑜𝑡𝑎𝑙 𝑚𝐿 ∗ 9.0𝑚𝐿 𝐶𝑢𝑆𝑂 = 0.036𝑀 𝐶𝑢𝑆𝑂 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛 = . 060 𝑚𝑜𝑙 𝐿 15 𝑡𝑜𝑡𝑎𝑙 𝑚𝐿 ∗ 12.0𝑚𝐿 𝐶𝑢𝑆𝑂 = 0.048𝑀 4. Using Excel or another graphing program, create a graph of absorbance (A) as a function of the copper(II) concentration ( M ) for each of the five known standard solutions you prepared. Your graph should include visible data points and the best fit line. Be sure to label axes with appropriate labels including units. Your graph is called a calibration plot (or standard curve) because it provides a relationship between absorbance and concentration for aqueous solutions of this compound. 5. What wavelength did you use when making your absorbance measurements? 700nm
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