pp_lab_report_glucose_762239

.pdf

School

Forsyth Technical Community College *

*We aren’t endorsed by this school

Course

169

Subject

Chemistry

Date

Dec 6, 2023

Type

pdf

Pages

4

Uploaded by alexalm0124

Report
Laboratory Report LABORATORY REPORT Activity: Blood Glucose Regulation Name: Alexa Lara Instructor: Tandeka Boko Date: 01.18.2023 Predictions 1. Plasma glucose levels will be highest 1-3 hours after the meal 2. Plasma ketone levels will be highest before the meal (fasting) 3. Plasma insulin levels will be highest immediately after the meal (0 hr) 4. Plasma glucagon levels will be highest 1-3 hours after the meal Materials and Methods 1. Dependent Variable plasma levels of glucose, ketones, insulin, and glucagon 2. Independent Variable food and beverage intake 3. Controlled Variables physical activity, caffeine and alcohol intake, gender, age, BMI 4. Why were physical activity and caffeine and alcohol intake the controlled variables? Because the amount of caffeine and alcohol intake can be controlled. And you can control the amount of time spent doing physical activities to. 5. What type of assay was used to measure plasma glucose and ketone levels? Enzymatic analysis 6. What type of assay was used to measure plasma insulin and glucagon levels? Radio immune assay Results Table 2: Glucose and Ketone Concentration in Blood Plasma Glucose (mmol/l) Ketones (μmol/l) Sub. 1 Sub. 2 Sub. 3 average Sub. 1 Sub. 2 Sub. 3 average Fasting 5.2 5.3 5.4 5.3 98 102 103 101 Immediately post meal 6.3 6.5 6.4 6.4 110 94 111 105 I hr post meal 6.2 6.2 6.4 6.3 61 59 51 57 2 hr post meal 5.6 5.5 5.5 5.5 60 76 72 69 3 hr post meal 4.8 4.7 4.6 4.7 57 52 69 59 Laboratory Report/ Alexa Lara/ Blood Glucose Regulation/ Tandeka Boko/ 01.18.2023/ Page [1] of [4]
Laboratory Report Glucose Concentration in Blood Plasma μmol/l 0 1.4 2.8 4.2 5.6 7 1 2 3 4 5 1. Fasting 2. Immediately post meal 3. 1 hr post meal 4. 2 hr post meal 5. 3 hr post meal Ketone Concentration in Blood Plasma μmol/l 0 22 44 66 88 110 1 2 3 4 5 1. Fasting 2. Immediately post meal 3. 1 hr post meal 4. 2 hr post meal 5. 3 hr post meal 1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest. 0 hour to 1 hour was the highest 2. When was plasma glucose concentration lowest? If values are similar for several time points, then give range of times when plasma glucose concentration was lowest. 3 hours post meal 3. When was plasma ketone concentration highest? If values are similar for several time points, then give range of times when plasma ketone concentration was highest. 0 hours post meal 4. When was plasma ketone concentration lowest? If values are similar for several time points, then give range of times when plasma ketone concentration was lowest. 1 hour post meal Table 3: Insulin and Glucagon Concentration in Blood Plasma Insulin (pmol/l) Glucagon (pmol/l) Insulin / Glucagon Ratio Sub. 1 Sub. 2 Sub. 3 average Sub. 1 Sub. 2 Sub. 3 average Fasting 68 51 75 65 37 34 35 35 1.9 Immediately post meal 76 102 93 90 35 36 34 35 2.6 I hr post meal 173 161 170 168 29 32 31 31 5.4 2 hr post meal 121 120 146 129 35 36 36 36 3.6 3 hr post meal 85 64 60 70 34 36 35 35 2 Laboratory Report/ Alexa Lara/ Blood Glucose Regulation/ Tandeka Boko/ 01.18.2023/ Page [2] of [4]
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help