Lab #7 Report

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School

University Of Arizona *

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Course

447

Subject

Mechanical Engineering

Date

Jan 9, 2024

Type

pdf

Pages

5

Uploaded by EarlMule3901

McKenna Nichols BME 447 Loeffler 25 October 2023 Chapter 8 Lab Report: Spectrophotometry Results Task 1: Hemoglobin Quantification with a Spectrophotometer Intensities Measured At Wavelength: 530 Concentration (mg/mL) Intensity Absorbance 0 12258.46 0 0.125 3389.73 0.55827 0.25 2701.61 0.65681 0.5 2683.69 0.06597 1 679.41 1.25630
Task 2: Hemoglobin Quantification with LED/PD Circuit Concentration (mg/mL) 𝑉 𝑜?? Absorbance 0 11.1 0 0.125 10.3 0.03249 0.25 9.6 0.0630 0.5 9 0.09108 1 8.1 0.13684 Task 3: Unknown Sample 𝑉 𝑜?? Absorbance Assumed Concentration (mg/mL) 9.9 0.04969 0.25
Discussion In the first portion of this lab, we began by making five solutions which each have different concentrations of hemoglobin. The concentrations we made are 1.0 mg/mL, 0.5 mg/mL, 0.25 mg/mL, 0.125 mg/mL, and 0.0 mg/mL. We then used the spectrophotometer to measure the intensities of these concentrations. We then used these intensity values to calculate the absorbance for each solution. The equation we used for absorbance is , where is 𝐴 = 𝑙𝑜𝑔( 𝐼 0 𝐼 ) 𝐼 0 the light intensity from the light source and is the light intensity passing through the material. 𝐼 Now that we have the absorbance, we graphed that in comparison to the concentrations. This graph can be seen in the results section above. Our results showed that there is a direct relationship between the absorbance of hemoglobin and the concentration of hemoglobin. This makes sense because as the hemoglobin concentration increases, the solution becomes less transparent. This means that more of the light is absorbed by the hemoglobin and less of the light passes through the solution. In the second portion of this lab, we used the same five solutions that we made for the previous section. To begin we had to wire the circuit that is shown to the right. From here, we measured the value of when 𝑉 𝑜?? the cuvette was filled with different solutions of different concentrations. We did this for the concentrations of 1.0 mg/mL, 0.5 mg/mL, 0.25 mg/mL, 0.125 mg/mL, and 0.0 mg/mL. We then used these values to find the absorbance for each solution. We used the 𝑉 𝑜?? equation where is the voltage output of the 0 mg/mL solution and is 𝐴 = 𝑙𝑜𝑔( 𝑉 𝑜??,0 𝑉 𝑜?? ) 𝑉 𝑜??,0 𝑉 𝑜??
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