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Essay about A Study on A. Nigra Seeds for Pharmaceutical Products

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Introduction Usage of natural antioxidants from plants and the other natural sources has been among the most important ingredients in food and pharmaceutical industries. Having the ability to defend against the reactive oxygen stress (ROS),Natural antioxidants can protect the human heath from a lot of diseaseses, they are Generally Recognized As Safe (GRAS) and the have less toxicity in industrial products compared to the artificial synthetic ones. Zingiberaceae is a family in the plant kingdom that has been highly investigated due to its versatile nature and high medicinal impact. The largest genus of Zingiberaceae family is Alpinia. In this paper , the antibacterial efficacy of hexane, ethyl acetate and methanol isolated from Alpinia…show more content…
The samples were then dried properly to remove the trace of remaining solvent. The extracts were subjected for vacuum drying in rotary evaporator. The free radical scavenging activity of seed extracts of A. nigra were determined using DPPH whereby solution of DPPH in 99.99% ethanol was prepared and was mixed with ethanolic solutions of n-hexane, ethyl acetate and methanol extracts from A. nigra seeds at determined concentrations. The mixture was shaken and incubated. Butylated hydroxyl toluene (BHT) as positive reference ethanol as negative reference were used. The absorbance was measured using multimode microplate reader. Each sample extract were mixed with dry KBr powders and their absorption spectra were obtained with a Perkin-Elmer FTIR spectrophotometer. The 1H spectra were recorded on a Varian 400 MHz FTNMR. Each extract sample were dissolved in CDCl3 , and the solvent signal was used for spectral calibration. Total soluble phenolic compound content from three different dilutions of A. nigra seed extracts were estimated according to the Folin–Ciocalteu colorimetric assay, using gallic acid as standard and the absorbance was finally measured. To generate the standard curve, different concentrations of gallic acid were used. Quantification of totall soluble phenolics in each extracts was determined from the gallic acid standard curve. The antibacterial activities of all the extracts were tested against Staphylococcus aureus
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