Alexander Flemming: The Antibacterial Enzyme

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Alexander Flemming was a scientist that discovered the enzyme lysozyme in 1923 when he accidently mixed the mucous fom the patient in the culture contain bacteria and the bacteria was dead. That is whee he took a closer look at the properties of the mucous from the patient and found the antibacterial enzyme. Lysozyme is one of many proteolytic ezyme foud in the body. It is a first defense system in the body that can destroyed bacterial cell wall. Lysozyme is very effective against gram positive bactreia because they have peptidoglycan cell wall and it is make the lysozyme very uneffective against bacteria that made of other other polysaccharide. Lysozyme mostly found in the bodily fluid such as salive, tears, human milk and also mucous. Newborn babies that fed with the…show more content…
It specifically catalyzing hydrolysis 1,4-beta-linkage between N-acetylmuramic acid and N-acetyl-D-glucosamine which are a very sturdy bond in the bacterial cell. This is where the lysozyme is targeted. In the mucous membrane, there is antimicrobial compounds such as lysozyme and secretory antibodies (IgA). Lysozyme can kill the bacteria but it less effective against the infection. Present of the lysozyme inhibit or kill the bacteria before they can colonized either in the mouth, eye or in other mucous secretion. Throughout this experiment, the lysozyme activity was determine using agarose lysoplate method. Using the sample collected the experiment was conducted within several days to see the clear zone, but the result obtained did not showed any clear zone even after added the coomassie brilliant blue stain. This may due to the plate bacteria inside the plate was dead when the sample was loadedin the well. There is possibility that bacteria was dead because the present of the bacteria in the plate cannot be seen and mybe the environment or the condition in the plate was not favourable for the bacteria to stay
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