Charles Gathathi
BIOL 2420.
Dr. Elizabeth Carrington.
Proteus mirabilis:
I. Introduction:
Proteus mirabilis is a Gram-negative, dimorphic bacillus and a facultative anaerobe bacterium. It is a motile member of the family Enterobacteriaceae. This bacteria colonies shows a unique characteristic called swarming behavior. Swarming occurs when the flagellate rods grow into long chain swarmer cells, the swarmers gather together forming “rafts” that swim together, growing into a complex biofilm. P. mirabilis virulence factors include swarming motility and urease production. Ref: Joan L, John F. microbiology: an evolving science New York 2009, Belas, Schneider and Melch. Journal of bacteriology Dec. 1998 p. 6126-6139.
Its scientific
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Diseases:
Statistically, about 90% of the proteus infections are caused by proteus mirabilis. It is an opportunist pathogen, which means it can cause diseases if it colonizes another place other than the gastrointestinal tract.
P. mirabilis is commonly associated with urinary tract infections. The infection begins when the organism moves from urethra to the urinary bladder. Urinary tract infections caused by P. mirabilis are mostly found in patients under long-term catherization. Other groups of patients that are at high risks of the diseases include; those patients with structural abnormalities of the urinary tract, patients with recurrent infections and patients whose infections were acquired in the hospital or long-term care facilities.
Proteus mirabilis infections accounts for 1%-2% of urinary tract infections in healthy women, 5% of nosocomial urinary infections and catheterization has a high prevalence of 20%-45%. The organism causes bladder infection and kidney infection. P. mirabilis produces large amounts of urease which splits urea into carbon dioxide and ammonia. This process increases the alkalinity of the urine and also it precipitates magnesium and calcium in the form of struvite and apatite crystals respectively. The results is the formation of renal stones.
Ref: Gus, Gonzales MD medical oncologist: proteus infections Medscape, Murray, Rosenthal, And Pfaller. Medical microbiology 7th edition 2013.
The objective of the investigation was to perform multiple experiments that would lead to the identification of an unknown bacteria. This bacteria was causing a urinary tract infection on a patient. A sample of the bacteria causing the infection was received and analyzed. The first step was to isolate the bacteria to insure that sample was free of contamination. A MAC plate, a PEA plate, along with a Nutrient agar plate where used to produce pure colonies by using the quadrant streak method on each plate. After a 24 hour incubation period the colonies where isolated. The bacteria collected was inoculated into slants, nutrient broth for cultivation. A sample from the MAC plate and the PEA plate were collected and gram
The organism adheres to the epithelial cells in its host by pilli. This opportunistic pathogen can affect people of all ages and specifically targets patients that have immune deficiencies, debilitating diseases, and infants in the NICU. Patients are at greater risk of contracting the organism if they have recently had a surgery, mechanical ventilation, central venous catheter, arterial catheterization, inhalation medication therapy, tracheal tubes, or have low apgar scores. It can cause a variety of diseases and infections such as urinary tract infections, meningitis, pneumonia, respiratory tract infections, keratoconjunctivitis, osteomyelitis, keratitis, endocarditis, cutaneous infections, and endophthalmitis. (Currey,
This condition is caused by bacteria. It is passed from an infected partner during sexual activity. This contact could be with the genitals, mouth, or rectal area.
In conclusion, after conducting previously mentioned biochemical tests in order to identify the unknown bacteria, became obvious that unknown gram negative was Proteus Vulgaris. Having eliminated all the bacteria that didn’t show expected results and confirming with such tests as catalase, glucose, indole, citrate, urea, and SIM, it was accurate to name the unknown gram negative. Furthermore, gram positive became obvious it was Streptococcus Pneumoniae. Having eliminated all the bacteria that didn’t show expected results and confirming with such tests as blood agar, catalase, and optochin and bacitracin tests, it was accurate to name the unknown gram positive. I have learned that it is extremely important to be to able to identify what kind
A Proteus infection may begin exogenously or endogenously. The mode of transmission for Proteus infections may be through direct or indirect contact. The pathogen is popular among hospitals; therefore, sources include hospital equipment, invasive procedures, hospital food and the hands of employees. It can be an endogenous infection when the normal intestinal flora is disrupted by antibiotics. This allows Proteus vulgaris to colonize and grow uncontrollably (NCBI, 1996).
array of aliments ranging from a mild rash to amputation, even death. Strains of the drug resistant pathogen
n.d.). P. Mirabilis is usually not pathogenic, however, once it enters urinary tract, lungs or wounds in the human body, it becomes pathogenic and will use blood vessels to travel to other areas of the body (Murphy, P. n.d.). P. mirabilis can cause urinary tract infections and kidney stones (EOL, n.d.). A urinary tract infection is an infection of the urinary system, which includes the kidneys, bladder, urethra and ureters (M. (2015, July 23). When you have a urinary tract infection you will have symptoms of red and strong-smelling urine, pelvic pain and a burning sensation while peeing (M. (2015, July 23). Urinary tract infections are treated with antibiotics (M. 2015, July 23). Kidney stones are hard, small masses made up acid salts and minerals that are formed inside the kidneys (M. 2015, February 26). When you have kidney stones you will have severe back pain, trouble urinating, cloudy urine, urinating more than normal and pink, red, or brown urine (M. 2015, February 26). There is no treatment for kidney stones, they have to pass on their own (M. 2015, February 26). This process is usually very painful, but usually do not cause any later damage (M. 2015, February 26). To make them tolerable, doctors recommend drinking water and pain relievers (M. 2015, February
Proteus vulgaris is associated with urinary tract infections, which if left untreated can escalate to complications such as sepsis in the blood. A fever is the most common symptom for an untreated UTI followed by: pelvic pain, sudden urge to urinate followed by a burning sensation and cloudy urine. To prevent being a victim, the number one prevention and transmission is to always wipe from front to back, drink plenty of fluids and emptying of
Lehne (2013) continues by stating that in nosocomial caused cystitis E. coli makes up only 50 percent of UTI’s. In hospital settings more common gram negative causative agents of cystitis are Pseudomonas, Proteus or Klebsiella pneumoniae. There is also a gram positive agent named Staphylococcus saprophyticus that makes up 10 to 15 percent of nosocomial UTI’s.
Pseudomonas aeruginosa is a gram negative, rod-shaped bacteria, almost all strains of P. aeruginosa are motile (single polar flagella). The bacteria is capable of adapting to and therefore thriving in many different ecological environments (from water and soil to plant and animals) it also can utilize a variety of organic compounds as food sources, giving it the ability to colonize in places where nutrients are limited. It can cause a range of infections from pneumonia to the most serious cystic fibrosis. P. aeruginosa can form a biofilm that allows the bacteria to form a resistance to antibiotics. To this day there are conferences, websites, and microbiologists committed to the research and discovery of P. aeruginosa in the fight to discover and learn more about the bacteria and ways to fight its biofilm.
The diagnostic of the urinary system is that a physician will test the urine for pus and bacteria by using a sterilize container to collect the sample. The Patient should clean the gentile area to prevent bacteria from getting in the gentile section this provide an accurate test for urinary tract infection. The test includes the searching for bacteria, white blood cells and red blood cells. The bacteria may be tested against different antibiotics to see the type of drugs that is used abolish the bacteria found in the urinary tract system. One of the test is also called the sensitivity test. Microorganisms such as chlamydia and mycoplasma can be detected with an elite bacterial cultures test. The test include an intravenous pyelogram it
Urinary tract infections (UTIs) commonly occur in the bladder (cystitis) or urethra (urethritis), but may also happen in the kidneys (acute pyelonephritis). UTIs occur more often in women than in men as women have a shorter distance from their urethra to anus, and also because their urethra is shorter than men’s giving the bacteria less of a distance to travel. Other risk factors may include sexual activity, especially if one has a new partner as they carry potentially new bacteria. Certain types of birth control, menopause, and those who use catheters are also at a greater risk of UTIs. Symptoms one may experience if they have a UTI are frequently urinating small amounts, urine that is cloudy and/or discolored (especially tinted pink or red
Urinary Tract Infections (UTIs) are a group of diseases that affect the organs in the urinary system such as the kidney, bladder and interconnecting tubes. The infection is often caused by bacteria and other micro-organisms which rarely originate from the bloodstream. The three most common types of UTI infection are Cystitis which infects the bladder, Pyelonephritis which infects the kidney and urethritis which is common in the urethra. Kidney infections are especially serious and may require immediate medical attention.
This condition is caused by an increase in bacteria in the urine. This increase can be caused by:
The relationship between bacterial infection of urinary tract infection with prostate specific antigen in patient's serum and the most common bacteria of urinary tract infection leading to increase the level of prostate specific antigen in serum. The UTI is the most common disease of the urinary tract and it is a major cause of morbidity in both the hospital and the community (2). They are caused by bacteria that are normally present in digestive system and on the skin around the rectum. These bacteria can pass through the urethra and up into the bladder (3).