Break Up Dna Write Up

In this experiment, DNA was extracted from human cheek cells using Gatorade, soap solution, and rubbing alcohol. To do this, Gatorade was swished in the subject’s mouth vigorously for 45 seconds, and then added to soap solution and rubbing alcohol. This mixture was left to sit in ice water for two to three minutes. It was hypothesized that when cheek cells were placed into Gatorade, soap solution, and rubbing alcohol, that the DNA would be extracted, forming a stringy substance. This hypothesis was supported by the results of the experiment. After adding the alcohol to the Gatorade and soap solution, a semi-translucent, fibrous substance was formed.

From cases such as OJ Simpson to Chandra Levy, DNA profiling also called DNA fingerprinting or DNA typing has played a major role in the criminal justice system. The law enforcement community uses DNA profiling to rule out or identify suspects. Unlike hair microscopy, bite mark comparisons, shoe print comparisons, and firearm tool mark analysis, DNA typing has been developed through massive scientific research and has undergone meticulous scientific evaluation (Innocence Project). DNA is a foolproof method of identifying a perpetrator of a crime.

This paper explores the history and some interesting facts about DNA. The last couple centuries have seen an exponential growth in our knowledge of DNA. The history of the DNA can be traced back to multiple devoted scientist. This article attempts to summarize, and review the basic history of DNA while providing some fascinating information about it.

At first a collection of blood and sperm samples from anonymous donors which are then combined into a mixture. The mixture is subjected for DNA extraction using chloroform, phenol and water. Pure DNA is dissolved in water. It is subjected to an enzyme, restriction that cuts the DNA fragments. The short DNA fragments are treated with alkali to make sticky ends.

Each human being has something called DNA. DNA is described as genetics and an extremely long macromolecule that is the main component of chromosomes and is the material that transfers genetic characteristics in all life forms. DNA constructs of two nucleotide strands coiled around each other in a ladder like arrangement with the sidepieces composed of alternating phosphate and deoxyribose units and the rungs composed of the purine and pyrimidine bases adenine, guanine, cytosine, and thymine. Each chromosome consist of one continuous thread-like molecule of DNA coiled tightly around proteins and contains a portion of the 6,400,000,000 basepairs that make up your DNA.

In this experiment we were meant to observe the transferring of DNA. There are many ways in which DNA can be transferred into an organism, for example; transformation, transduction, and conjugation. In our experiment we used

Aram, a 16-year-old trainee hairdresser from Keyworth in Nottinghamshire, was abducted, raped and strangled on 30 October 1983 – five years before the careless driver was born. (Her murder was featured in the first ever episode of Crimewatch in 1984.) The police had a few circumstantial leads to go on: a stolen red Ford Fiesta; a handwritten message from the killer saying they'd never catch him; a paper towel recovered from the toilet of a pub, the Generous Briton, where shortly after the murder a man with blood under his fingernails had eaten a ham sandwich, drunk a pint of orange juice and lemonade, and told the landlady an unlikely story about having driven up the M1 to see some friends who weren't in. Twenty thousand people were interviewed in the course of the investigation, but the killer

There are many sources of DNA for testing. Blood is one of the key sources, though the surface on which a bloodstain is found can profoundly affect the ability to successfully perform an analysis. In addition, bloodstains may be mixtures of blood from two different people and can produce DNA profiles that are more complex than those from a single individual. Ironically, DNA profiling may be the only way to determine if a given stain is a mixture. Semen stains are the most common evidence to be submitted for DNA analysis, which is not surprising since the cases in which DNA testing has been used the most often are rapes. DNA can also be extracted from tissues (taken at autopsy), hair roots, saliva, and in rare instances, urine.(4) It is important to note that only a miniscule amount of DNA is needed for analysis. For example, the amount of DNA found at the root of one hair is usually sufficient. Environmental factors also play a role in determining whether a particular sample of DNA can be utilized. Moisture, sunlight, bacterial action and heat are detrimental to the DNA. Depending on the intensity and combination of these conditions, survival of the DNA is measured in weeks or months. Even so, DNA in usable amounts can

Do you know what the DNA of relationships is? Well, I finally figured it out. After reading the book DNA of Relationships, according to author Dr. Grey Smalley, the three codes are: you are made for relationships, you are made with the capacity to choose, and you are made to take responsibility for yourself (Smalley 11). While reading this book I came across a few unnoticed strengths and weaknesses of how I handle my own relationships. I was able to point out some expectations I look for in a relationship, as well as the boundaries I set in result of fear. Although I have was not able uphold past relationship because I was not aware of these fears, I known have the ability to improve my relationships.

On October 12 2017, the class was set to collect DNA samples from a strawberry. Placing the strawberry in a ziplock bag and zip up the bag. Squish the strawberry into a liquid. Add 10 ml of the buffer (salty, soapy water) into a 10ml graduated cylinder using a transfer pipettes. Using the 10ml graduated cylinder, open up the ziplock bag and pour the 10 ml buffer with the squished up strawberry then zip up the ziplock bag. Mix it by moving it around using your fingers, but be cautious about how much bubbles are created. Add the a funnel lined with a cheesecloth onto a test tube (filtration apparatus) and filter the new berry-buffer solution in the filtering apparatus into a test tube. When finished with adding the new berry-buffer solution

7. What techniques are used to combine DNA from one species with DNA from another?

DNA editing was discovered thanks to the clash between bacteria and viruses. Bacteria and viruses have been fighting each other since life on Earth began. During an invasion of a cell, the virus inserts its own genetic code(RNA) into the bacterium and takes it over to use as a factory to replicate itself. The bacterium, meanwhile, tries to resist but ends up failing most of the time because it has weak, and insufficient tools to protect itself. Some bacteria however, through natural selection or chance, survive the attack and save part of the virus’s genetic code(Kurzgesagt, 2016). The RNA of the virus is then saved in the bacterium’s own genetic code in a DNA archive

Let the test tube sit undisturbed for 2 - 5 minutes. You should begin to see air bubbles form at the boundary line between the ethanol and the filtered fruit solution. Bubbles will form near the top, and you will eventually see the DNA float to the top of the ethanol. Gently insert the stir stick into the test tube. Slowly raise and lower the tip several times to spool and collect the DNA. If there is an insufficient amount of DNA available, it may not float to the top of the solution in a form that can be easily spooled or removed from the tube. However, the DNA will still be visible as white/clear clusters by gently stirring the solution and pushing the clusters around the top. Post-Lab Questions What is the texture and consistency of the DNA DNA is viscous and greasy. Why did we use a salt in the extraction solution High salt makes DNA less soluble in water. In order to dissolve, the water needs to interact with the DNA. Since DNA is quite large, it needs to interact with lots of water for this purpose. When you add salt, the water preferentially interacts with the salt (its small, and can move around in solution easier than the DNA can). This makes it so there is less water available to interact with the DNA and it becomes less soluble. Is the DNA soluble in the aqueous solution or alcohol DNA is less soluble in an alcohol such as isopropanol than it is in water. This is because alcohols are non-polar, whereas water is polar. The polar

material of some viruses. RNA molecules are like DNA. They have a long chain of

The chemical and reagents used for the extraction and quantitation of DNA were: Plant DNAzol (0.3ml/0.1g), 100% ethanol (100%: 0.225 ml/0.1 g, 75%: 0.3 ml/0.1 g), Chloroform (0.3 ml/0.1 g), Plant DNAzol-ethanol solution: Plant DNAzol, 100% ethanol (1:0.75 v/v), TE buffer (10 mM Tris, 1 mM EDTA pH 8.0), 1.2% agarose gel (Agarose, 1X TAE buffer), 6X loading buffer (glycerol, Tris/EDTA pH 8.0, ethidium bromide), .25X TAE buffer, Restriction enzymes and Restriction endonuclease buffers. All the chemicals used were quality grade. The restriction