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Dehydrogenation Reaction Lab

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The purpose for the experiment was to perform a dehydrogenation reaction. The experiment showed when ADH was the most productive with four substrates ethanol, butanol, propanol, methanol and different temperatures and pH levels. In order to perform a dehydrogenation reaction there should be a product and a substrate. There are two protons that were removed one proton went into the reduced form NADH that formed and the other proton was released which in return makes the reaction. The problem under the experiment was determining which temperature and pH level was most affective at one substrate for the enzyme ADH and what substrate was most effected by
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