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Eukaryotes Lab Report

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Introduction/Background: Transcription in eukaryotes is one of the most vital processes of life that involves a highly controlled and regulated systematic series of events that is mediated through various key factors. The process of transcription occurs when the genetic information stored within DNA becomes activated through the synthesis of complementary mRNA and is thus regulated by RNA polymerases. There are three types of RNA polymerases that distinctively transcribe a specified set of genes. RNA polymerase I and III transcribe genes that have terminal products such as ribosomal subunits, tRNA and small nuclear RNA. For example, RNA polymerase I is located in the nucleolus of eukaryotic cells and is responsible for transcribing 18S, 5.8S, …show more content…

The TATA box is located at a regulatory region of the DNA and is positioned 25 nucleotides upstream from the starting point of transcription. Once TBP is attached, it allows TFIID to bind. The recruitment of three additional TFs, TFIIA, TFIIB, and TFIIF is necessary to prepare the DNA for the binding of RNA polymerase II, thus forming the protein complex. RNA polymerase II must arrive with TFIIE and TFIIH to gather around the TATA box and form the pre-initiation complex. TFIIH utilizes the energy from ATP to unwind the double helical structure of DNA and widens the minor groove. RNA polymerase II becomes phosphorylated by TFIIH and causes a conformational change that initiates RNA synthesis and elongation begins (Berg JM,.et al, 2002). However, preRNAs need to be processed before translation takes place through splicing, capping, and polyadenylation, which ultimately form the mRNA …show more content…

al, other diseases associated with myc abnormalities include diffuse large B-cell lymphoma and B-cell lymphomas that together form a clinically aggressive phenotypic disease. Research suggests that there is a spectrum of associated lymphomas that are driven by mutations, rearrangements, and chromosomal abnormalities, however, when these are joined with BCL2 or BCL6 rearrangements the outcome is the onset of highly aggressive diseases such as DHL or THL. Data suggests that due to profiling, there is a particular pattern of expressed genes when myc is amplified in B-cell lymphoma when compared to other types of lymphomas, which supports that even though myc is involved in promoting the expression of targeted genes during transcription, these target genes differ when it comes to other diseases associated with myc. Current treatments are proven to fail when the disease is driven by myc. Therefore, better targeted treatments are necessary when trying to combat myc driven

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