Gutta Percha Lab Report

Three ways to assess water are positive/negative, membrane filtration and IDEXX Colilert and Enterolert assays. For membrane filtration, petri dishes and an incubator are used. For IDEXX coliert powdered medium is used for total coliform and E. Coli, while Enterolert media is used for enterococci. Also an incubator, colilert trays are used. IDEXX is more expensive than membrane filtration.

9. After the 48 hour time period the inhibition of the Escherichia coli bacteria around the disinfectant disks was measured. A ruler was used to measure the clearing from the edge of the disk to the perimeter of the clearing. Irregularities in the clearing were not included in the area of clearing.

When determining which bacteria I wanted to use for this experiment I had to decide on E.coli bacteria which is gram negative and staphylococcus a gram positive bacterium. These were chosen because they are safe enough to grow in a college laboratory and were supplied by the technician. Gram negative bacteria has an outer membrane making it more resistant to antiseptics and antibiotics, it also makes it more fatal to the human host it is inhabiting. Whereas gram

A male Betta is to be placed on the opposite side of the bowl, so that they can see each other but no contact. The fish were observed for two minutes. After the two minutes passed, the barrier was lifted and observations were recorded for one minute before the male was removed from the bowl. This was then repeated with the female Betta. These two constitute one trial. There was a total of three trials, so both the male and female were in the primary bowl thrice

For this lab 6 male Acheta domesticus were isolated in plastic containers, each was placed in a container that was 5 cm height and had 5.5 cm radius. Each of these containers was punctured with 10-15 minuscule holes for breathing on the lid and 5-8 holes on the sides. Inside of each container a damp paper towel was placed on the bottom and 2 pieces of food were placed on the paper towel. The Acheta domesticus were kept isolated in their personal containers for 7 days. At the end of these 7 days we split the 6 A. domesticus into 3 groups of 2. One of the two was marked with a paint pen, this marked male was the “intruder”. The remaining food was removed from the containers. The “intruder” is then placed into the container that holds the other

We selected two locations for sample collection: for Site 1 a phone was selected, and Site 2 a desk. One agar plate was labeled “Control” with our group identification number. A sterilized swab was dipped into a test tube with sterile water to wet the cotton on the swab. The culture from the swab was transferred to the surface of an uncultured nutrient agar plate using a zigzag motion. This is to determine if there are any bacteria in

The results showed that the strong oxidative solutions did not allow for the growth of bacterial colonies where the solution containing alcohol allowed for the growth of 264 colonies. Since there was no growth in either of the petri dishes containing hydrogen peroxide or bleach, it could not be concluded which, if either, chemical agent was the superior disinfectant. (See figure 1).

The simulated gastric solution consisted of distilled water containing 0.2% NaCl with its pH adjusted to 1.5 by 5 M HCl; the solution was filter-sterilized using a 0.2 mm filter (Cook et al. 2011; Mokarram et al. 2009; Rao et al. 1989). The assay was initiated by transferring the produced beads (uncoated, coated) to 9 mL of simulated gastric solution; the initial cell concentration was approximately 8.8× 1013 CFU/mL and 2 -6 × 1012 (uncoated, coated beads). The suspension was incubated at 37° C; samples were collected at 0, 60, and 120 min and following the incubation, the beads were removed. Viable cells of each bacterium were then enumerated in triplicates using method described in Section 2.5 and the counts were expressed as mean log CFU/mL.

The aims of this experiment were to investigate the inhibitory power of nisin on Listeria innocua and determine the minimum inhibitory concentration (MIC) of nisin on this bacterium. This was evaluated by inoculating a sterilized slice of mozzarella cheese with a known concentration of L. innocua and nisin. Then after incubation at 7°C for 5 days, the log10 reduction value was calculated. Nisin’s effect on L. innocua’s growth was also evaluated via microdilution, with the goal of determining the MIC. The bacteria grew to a much higher concentration on the mozzarella cheese after being exposed to nisin and incubated at 7°C for 5 days and the microdilution procedure was unable to confidently determine the MIC of nisin on L. innocua. These results

The pre-disinfection of the lab bench showed colonies of bacteria and mold, however, post-disinfection showed neither bacteria nor mold. Because of the smooth surface of the lab bench, when we cleaned the area with disinfectant, the microorganisms were wiped out completely. This not only showed the effectiveness of disinfection on smooth surfaces, but it also demonstrated that the disinfection of lab bench is important task of the lab procedure. The disinfection of lab bench can reduce the risk of potential contamination during the lab when handling microorganisms. Disinfection of the area which is another way to practice aseptic technique will prevent contamination of the samples by other microorganisms, and it will also prevent any microorganisms to leave out from the lab

Birinapant (TL32711) is a bivalent SMAC mimetic. Apoptosis resistance acquisition is a fundamental event in the development of cancer. Among the mechanisms is the dysregulation of inhibitor of apoptosis (IAP) proteins regulated by endogenous IAP antagonists such as SMAC. Drugs mimicing the SMAC have been designed to overcome IAP-mediated apoptosis resistance of cancer cells.

The purpose of this lab is to observe bacterial growth between a water fountain and door handle

Nutrient Broth and Nutrient Agar were used to inoculate bacteria taken from different surfaces. Nutrient agar plate was inoculated with a sample taken from skin surface. A sterile cotton swab was first immersed on sterile water, then, rubbed against the skin with swirling motion and transferred to an agar plate by rubbing

Although bacterial infections are one of the main problems in the aquaculture industry, very few studies regarding the intestinal response to bacterial pathogens after natural infections can be found in the literature. Additionally, the number of studies performed in the laboratory under controlled conditions is low, prompting the need for more in-depth experiments to determine the role of GALT in bacterial infections.

Knowing the conditions agar-degrading bacteria accommodate in situ, it is possible to approximate similar conditions in vitro. Leon et al achieved this by spreading samples of sea water on agar plates containing 0.25% casein hydrolysate, 0.05% yeast extract, 2.5% NaCl, 0.06% NaH2PO4, 0.5% MgSO4, 0.002% FeSO4∙7 H20, 0.01% CaCl2 and 1.5% Agar with a pH of 7.25 and incubating them for 48 hours at 25°C(6). After 48 hours, single colonies that had made small pits in the agar (thus indicating the presence of agarase) were inoculated onto new plates with the same conditions as above. The aim of this selective and differential process is to ensure a pure culture, as non-agarolytic bacteria should not be able to survive the first plate, let alone a second one. This inoculation