The purpose of this experiment was to determine whether spinach leaves would produce more CO2 if spinach was under blacklight, versus in the the dark. It was hypothesized that if spinach was put under blacklight, then spinach would produce less CO2 because there would be some light energy entering the plant, and that would cause the spinach to photosynthesis. This would decrease the rate of CO2 production. The data did not support this hypothesis. This investigation was valuable because it showed that respiration was still happening in the dark, which can lead to other investigation. This is also important because this showed the effects of different wavelengths of light on the rate of CO2 production in plants, and info into what might be happening
Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.
This lab was conducted to discover which level of light (measured in lux) would make Spinacia oleracea leaf disks float the fastest. Lux (lx) is latin for light, and is the unit of measurement for light in the International System of Units (Rouse 2011). Light plays a key role in the process of photosynthesis and in this we are essentially measuring the rate at which Spinacia oleracea leaf disks convert a bicarbonate solution into oxygen. The oxygen then would bubble up, allowing
The leaf with the highest rate of photosynthesis was the darkest leaf, the purple cabbage. The leaf with the lowest rate of photosynthesis was the lightest leaf, the iceberg lettuce. We can conclude that the rate of photosynthesis was much higher in the purple cabbage because darker leaves contain more chloroplasts. Therefore, photosynthesis occurs much more quickly. Notably, these results support the stated hypothesis. An error that might have affected the data as a whole was the fact that the calculated rate of photosynthesis for the iceberg lettuce was negative. In addition to this, a few of the other percentages decreased as time passed. This means that the chloroplasts would have been going through cellular respiration. This inconsistency might have transpired from a mechanical error within the spectrophotometer. The control tubes, the “boiled” and “dark” tubes, of all 3 leaves were necessary in order to determine if the chloroplasts were the organelles of the cell that were causing one leaf to photosynthesize quicker than another because it was imperative for the chloroplasts to be exposed to light and temperatures that would not denature them. Therefore, we are able to conclude that the chloroplasts located within the leaves were the organelles that were causing the varying rates of photosynthesis in this
Photosynthesis is the conversion of light energy to chemical energy into sugars. It is the process in plants that uses carbon dioxide, water, and sunlight from its surroundings and releases oxygen as a byproduct (6H2O+6CO2+light energy -> C6H12O6+6O2). Photosynthesis is required for plants because they are autotrophs, organisms that make their own food. Plants require a specific environment that is ideal to them to be able to carry out the process. Environmental conditions can either increase or decrease the rate of photosynthesis. Things like colors of light, pH, and temperature can all affect the rate of photosynthesis in plants.
The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.
-Measuring the pH of a solution (such as in the lab we had) could also help determine the rate of photosynthesis. You would need a much more specific pH meter, but generally, if pH goes down, the level of CO2 is higher, meaning more cellular respiration. Higher pH means there’s less CO2, so more photosynthesis.
My lab partners and I performed an experiment that involved placing spinach disks into separate cups of distilled water (dH2O) and 0.2% sodium bicarbonate (NaHCO3) solution to examine photosynthesis in leaf tissue (Department of EEB, 2015). Discovering that the spinach disks quickly floated to the top of the 0.2% NaHCO3 solution and not in dH2O, we wondered if varied concentrations of carbonation would affect the rate of photosynthesis (PS). We tested this by halving the 0.2% NaHCO3 solution (using equal parts dH2O and 0.2% NaHCO3 solution to make 0.1% NaHCO3 solution). I hypothesize that if the spinach disks are placed in the 0.1% NaHCO3 solution, then they will have a slower PS compared to the disks placed in 0.2% NaHCO3. CO2
The rate of photosynthesis is affected by environmental factors like light intensity, light wavelength, and temperature. This experiment will test the
To measure the rates of photosynthesis by measuring the change in CO2 levels in the air that is in the LeafLab chamber.
13) If you ran the same experiment without passing light through a prism, what would you predict?
This experiment demonstrates the effects of pH on the rate of photosynthesis by examining the behavior of leaf disks in different pH solutions under light. In this experiment, we used five different pH levels: pH 5, pH 6, pH 7, pH 8 and pH 9. These solutions were created using a combination of hydrochloric acid and sodium hydroxide. Spinancia olcerea or spinach, leaves were used in the experiment to examine the effects of pH on the rate of photosynthesis. The rate of photosynthesis was measured by counting the number of leaf disks that rose to the surface of the solution after each minute. In acidic solutions, the rate of photosynthesis increased while in basic solutions, the rate of photosynthesis decreased.
In this lab, students did an experiment to figure out the amount of oxygen and carbon dioxide being produced during photosynthesis and respiration. First, they did an experiment in the light using spinach leaves. They put the spinach leaves in the chamber and turn the light on and start collecting the data in the computer. The rate of production of oxygen was high because of photosynthesis, however without light there were not photosynthesis happening, that is why there was more carbon dioxide being produced because of cellular respiration. The level of carbon dioxide increases steadily when the leaf is in the dark. The plant’s mitochondria are producing carbon dioxide gas as a by product of cellular respiration (which uses glucose
We had three sets of ten spinach chads that were submerged in CO2 water and put under different wattages of light: forty, seventy-five, and one hundred . Chads under the forty wattage only had six rise out of ten, chads under the seventy-five wattage light had all ten rise, and chads under the one hundred wattage light had all ten rise under ten minutes.
The purpose of this experiment was to take spinach leaves and extract the chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used, starting with the least polar, to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography.
The Effect of Light Intensity and Temperature on the Rate of Photosynthesis Aim The aim of my experiment is to determine whether intensity of light and temperature would affect the rate of photosynthesis in a plant. To do this, I will place a piece of pondweed in varying light intensities and temperatures, and observe the amount of oxygen being given off. I am using pondweed because of its unusual quality of giving off bubbles of gas from a cut end, when placed in water. Introduction Photosynthesis occurs only in the presence of light, and takes place in the chloroplasts of green plant cells.