For this experiment, student acquired two clean 250-mL Erlenmeyer flasks and 75-mL glass tubes as well as labeled one tube as Tube #1 the second tube as Tube #2. The student utilized the test tubes as photosynthesis compartments. Next, student used 225mL of tap water to fill each Erlenmeyer flask previously attained. Student used 75mL of treatment solution to fill Tube #1 and Tube #2 and placed both tubes in one of the water filled 250mL Erlenmeyer flasks, the water acted as a control variable for temperature change throughout the experiment. Student acquired 2 aquatic plants from the front bench and poured water from the container, which held the plants. It was mandatory for the aquatic plants to be composed of healthy, green leaves and …show more content…
Student crucially maintained the plant stem immersed and inserted the scissors into Tube #1 to cut off 0.5cm in the internode between the leaves of the stem and not where the leaves and stem attach, and repeated this step for Tube #2. Following, student inserted a rubber stopper in both tubes (including the attached bent pipette, syringe, and needle) and insured there were no air bubbles in the test tube by pushing the stopper into each tube. Student also cleaned the expected excess solution that overflowed from the test tube as the stopper was placed. Student allowed the solution to migrate into the bent pipette. After full insertion of the stopper, student used the syringe and needle to extract solution from each tube, setting the amount of solution in the bent pipette to “0”. Student positioned each Erlenmeyer flask containing the test tubes approximately 15cm in front of the lamp and turned on the lamp. Student left the plants to equilibrate for 10 minutes and noticed air bubble formation at the cut end of the stem. Using the syringe and needle, student altered the amount of solution in the pipette back to “0” at the end of the equilibration period and in five minute intervals, timed the gas production and recorded readings
The purpose of this experiment was to investigate the effects of light intensity on the rate of photosynthesis in a Moneywort plant. By observing the plant in distilled water mixed with sodium bicarbonate, different light bulbs were targeted onto the plant. The measurement of the amount of bubbles present on the plant during the trial of the experiment enabled us to identify the comparisons between the activity of the light and the process of photosynthesis.
Each section we added ( ml) of liquids. The null hypothesis that the growth of plants will be at its optimal value with tap water having a neutral Ph. The alternative hypothesis that there will be no change on the growth of plants in different liquids. Introduction:
There are many procedures during this lab and many materials needed for an accurate analysis of data. First, fill a 100 mL graduated cylinder with 50 mL of water. Add 25 germinating peas and determine the amount of water that is displaced. Record this volume of the 25 germinating peas, then remove the peas and put those peas on a paper towel. They will be used for the first respirometer. Next, refill the graduated cylinder with 50 mL of water and add 25 non-germinating peas to it. Add glass beads to the graduated cylinder until the volume is the same to that of germinating peas. Remove the beads and peas and put on a paper towel. They will be used in respirometer 2. Now, the graduated cylinder was filled once again, determine how many glass beads will be require to reach the same volume of the germinating peas. Remove the beads and they will be used in respirometer 3. Then repeat the procedures used above to prepare a second set of germinating peas, dry peas and beads, and beads to be used in respirometers 4,5,and 6, the only difference is the temperature of the water.
Experiment 1 (Assignment 3): Using sciccors, leaves from the Geranium plant were cut (Plant A was the bigger leaf and Plant B was the smaller leaf). Then begin to heat up the hot plate to boiling temperature of 100℃. Next one beaker was filled with ⅔ of water and another beaker was filled with ⅓ alcohol. Place the beaker of water onto the hot plate until boiling. To speed up the boiling process put boiling chips into the beaker. Then put Plant A (the leaf exposed to air) into the boiling water for 3-5 minutes. After time is up, using tongs, place Plant A directly into the alcohol solution for another 3-5 minutes. When time is up, take out Plant A and place it into a clean petri dish. Once the plant is properly placed, cover the leaf completely
Plants need specific conditions to grow. In this experiment plant will be put in hypertonic solutions. Three out of the six plant will be watered with a salt and water solutions. Watering the plants in the solution will place them in a hypertonic solutions, making the water leave the cells to diffuse (in this case) the salt. “Photosynthesis is the process in which plants use water, carbon dioxide, and light energy to make glucose and oxygen.”(PHOTOSYNTHESIS). If water is needed for
The rate of photosynthesis is affected by environmental factors like light intensity, light wavelength, and temperature. This experiment will test the
The volume of a small test tube and a thin-stemmed pipet were determined in this section of the lab. Water was poured into a small test tube until the water reached the very top edge of the test tube. The test tube was then emptied into a plastic 25 mL graduated cylinder and volume was measured and recorded into data table 3. A think-stemmed pipet was completely filled with water. Drops were carefully counted and emptied into the empty plastic 25 mL graduated cylinder until the water level reached 1 mL. The number of drops in 1 mL was recorded into data table 3. The thin-stemmed pipet had a total volume of 4 mL and that was also recorded into data table 3.
The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.
The next step in this lab is to rinse the Erlenmeyer flask with distilled water down the drain and then repeat the experiment, this time adding 10 ml of 0.10M KI and 10 ml of distilled water to the flask instead. The flask should again be swirling to allow the solution to succumb to the same temperature as the water bath and once it has reached the same temperature, 10 ml of 3% H2O2 must then be added and a stopper must be immediately placed on the flask and recording should then begin for experiment two. After recording the times, the Erlenmeyer flask must then be rinsed again with distilled water down the drain. After rinsing the flask, the last part of the lab can now be performed. Experiment three is performed the same way, but instead, 20 ml of 0.10 ml M KI and 5 ml of distilled water will be added and after the swirling of the flask, 5 ml of 3% H2O2 will be added. After the times have been recorded, data collection should now be complete.
This experiment demonstrates the effects of pH on the rate of photosynthesis by examining the behavior of leaf disks in different pH solutions under light. In this experiment, we used five different pH levels: pH 5, pH 6, pH 7, pH 8 and pH 9. These solutions were created using a combination of hydrochloric acid and sodium hydroxide. Spinancia olcerea or spinach, leaves were used in the experiment to examine the effects of pH on the rate of photosynthesis. The rate of photosynthesis was measured by counting the number of leaf disks that rose to the surface of the solution after each minute. In acidic solutions, the rate of photosynthesis increased while in basic solutions, the rate of photosynthesis decreased.
Duckweed is a small aquatic plant that is able to grow rapidly, making it the ideal specimen for our experiment. It is hypothesized that altering the amount of light received by duckweed will alter its photosynthetic rate. It is predicted that a lower light intensity will lower the rate of growth in duckweed.
Elodea Canadensis, also referred to as American waterweed or common elodea, is an aquatic plant that is usually found in lakes, ponds or even rivers. Elodea helps support aquatic life such as young fish and amphibians by providing shelter and can be consumed as food by ducks and beavers. Elodea, like many other plants, relies upon photosynthesis to acquire its energy and to make food. To determine the importance of light energy in the process of photosynthesis, two Elodea plants were placed inside CO2 saturated water and exposed to different light intensities: one was under full light exposure while another was covered with mesh clothing to reduce the amount of light that it received; the solutions to this plants were then neutralized and compared to a control group which had no Elodea to see the amount of CO2 that each would have left after a period of an hour. The yielded results indicated that under high intensities of light, the Elodea plant photosynthesized and respired at the same rate; and while it was covered, it photosynthesized more because of an indicative decrease in CO2 levels. Therefore light energy is an essential aspect that affects the amount of CO2 that a plant can use in the process of photosynthesis.
This lab will be driven by the research question, what is the effect of temperature (at 5oC, 15oC, 25oC, 35oC and 45oC) on the photosynthetic rate (measure of oxygen bubbles over a period of time) of Elodea (pondweed)?
However, the photosynthetic process can be affected by different environmental factors. In the following experiment, we tested the effects that the light intensity, light wavelength and pigment had on photosynthesis. The action spectrum of photosynthesis shows which wavelength of light is the most effective using only one line. The absorption spectrum plots how much light is absorbed at different wavelengths by one or more different pigment types. Organisms have different optimal functional ranges, so it is for our benefit to discover the conditions that this process works best. If the environmental conditions of light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will increase with average light intensity and under the wavelengths of white light which will correspond to the absorption spectrum of the pigments. The null hypothesis to this would be; if the environmental conditions light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will decrease with average light intensity and under the white light which will correspond to the absorption spectrum of the pigments.
Each treatment number represented a specific treatment, treatment 1 was the control, treatment 2 was the N-deficient, treatment 3 was he P-deficient, treatment 4 was the K-deficient and treatment 5 was the water treatment. Starting from the bottom of the container there were several layers; folded paper towels, cotton, another layer of folded paper towels, 30 mL of the treatment, evenly spread 10 mL of rye seeds, folded paper towels and 20 mL of the same treatment. The containers were placed under the fluorescent light for two weeks. After two weeks, using the dissecting probe remove 20 plants from the middle, left and right corners from each growth container. The seed and root was removed using scissors, leaving the shoot behind. The shoot was then placed on the scale and weighed for shoot biomass, in milligrams, which was converted to grams by multiplying 1000, and the shoot length was recorded in centimeters. The shoot’s biomass and length were transferred to the Data Sheet and to a table on Excel. Two individual graphs, shoot’s biomass and length, were made on Excel after calculating the statistical data.