INTRODUCTION Food additives have been used for centuries to help preserve foods, improve flavor and texture, and enhance the nutritional value in food. The Food and Drug Administration (FDA) has a list of over 3,000 food additives that are safe for consumption by humans. (1)
The first lab experiment conducted used food additives to compare the physical and chemical properties of three different thickening agents: corn starch, modified food starch, and guar gum. Guar gum is a thickening agent, but also an emulsifier. Emulsifiers are used to keep ingredients from separating. Corn starch is a flavorless powder that comes from corn kernels, and has caloric value which is made up of mainly carbohydrates. Modified food starch is a chemically
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Sample 1 was frozen orange juice from concentrate, without pulp. Sample 2 was 100% juice with pulp, not from concentrate. Sample 3 was organic 100% orange juice blended with concentrate.
The final experiment was conducted to understand how enzymes are used in food, specifically cheese, production. Enzymes are proteins that act as a catalyst and bring about chemical reactions. (4) The enzyme Rennin, which comes from the lining of a cow’s stomach, causes the protein in milk to coagulate. This coagulation is what forms curds in order to make cheese. In the production of cheese, milk is treated with buttermilk to lower the pH. Before being treated, the pH of milk is around 6.7 which makes it slightly acidic. The range of pH for milk used to make cheese would be between 4.1 to 5.3. (5)
METHODS
In the first experiment, each group of students was given 2 sample vials, 20 mL of vinegar, and 4 mL of vegetable oil. Also weighed out using weight boats was 0.3g of cornstarch and 0.3g of modified food starch. Using a pipette, the student added 10 mL of vinegar and 2 mL of vegetable oil to each vial. Both vials were labeled and shaken vigorously for 20 seconds. Observations were made and recorded, as shown in Table 1. After this, the students obtained 2 more vials and added the same amount of vinegar and vegetable oil to each one. Guar gum was weighed out into two separate containers, then 0.2g was added to one vial, and 2.0g was added to the other. The students once again shook
In this lab or experiment, the aim was to determine the following factors of enzymes: (1) the effects of enzymes concentration the catalytic rate or the rate of the reaction, (2) the effects of pH on a particular enzyme, an enzyme known and referred throughout this experiment as ALP (alkaline phosphate enzyme) and lastly (3) the effects of various temperatures on the reaction or catalytic rate. Throughout the experiment 8 separate cuvettes and tubes are mixed with various solutions (labeled as tables 1,3 & 4 in the apparatus/materials sections of the lab) and tested for the effects of the factors mentioned above (concentration, pH and temperature). The tubes labeled 1-4 are tested for pH with pH paper and by spectrophotometer, cuvettes 1a-4a was tested for concentration and cuvettes labeled 1b-4b was tested for temperature in four different atmospheric conditions (4ºC, 23ºC, 32ºC and 60ºC) to see how the enzyme solution was affected by the various conditions. After carrying out the procedures the results showed that the experiment followed the theory for the most part, which is that all the factors work best at its optimum level. So, the optimum pH that the enzymes reacted at was a pH of 7 (neutral), the optimum temperature that the reactions occurs with the enzymes is a temperature of 4ºC or
Amylase experiment # 2 was done to see how the pH affected the efficacy of the enzyme. First we collected all of the materials that were necessary to make this experiment. We needed five clean test tubes, the following standard solutions, 1% Starch Solution pH 3,1% Starch Solution pH 5,1% Starch Solution pH 7,1% Starch Solution pH 9,1% Starch Solution pH 11
Enzymes are types of proteins that work as a substance to help speed up a chemical reaction (Madar & Windelspecht, 104). There are three factors that help enzyme activity increase in speed. The three factors that speed up the activity of enzymes are concentration, an increase in temperature, and a preferred pH environment. Whether or not the reaction continues to move forward is not up to the enzyme, instead the reaction is dependent on a reaction’s free energy. These enzymatic reactions have reactants referred to as substrates. Enzymes do much more than create substrates; enzymes actually work with the substrate in a reaction (Madar &Windelspecht, 106). For reactions in a cell it is
Cornstarch was the next powder experiment on. Cornstarch is a white, soft powder generally used as a thickening agent in liquid-based foods such as soups or sauces. When mixed with lower temperature liquids, it forms a past/slurry. As the starch is heated, the molecular chains unravel, allowing them to collide with other starch chains to form a mesh, thickening the liquid (Starch gelatinization). When mixed with a fluid, cornstarch can make a non-Newtonian fluid, for example, adding water creates Oobleck while adding oil creates an Electro rheological fluid (a suspension of extremely fine non-conducting particles which can be up to 50 micrometers in diameter) (Cornstarch).
Most Americans love the low cost, prepackaged, great tasting food that is convenient to buy and prepare. These prepackaged or processed foods are normally made to be faster, cheaper and usually taste great but they are loaded with bizarre, unpronounceable chemicals that, if you saw them in their pre-processed state, you would never consider putting in your mouth. Emulsifiers, preservatives, colorants, stabilizers, artificial sweeteners, texturizers and even bleach can be found in most processed foods. On average, Americans spend 90% of their food budget on these types of foods which is found to contribute to the leading causes of obesity, auto immune disease, diabetes and even cancer.
The purpose of this experiment was to find out the nutrient content of unidentified food samples and to use chemical reagents to see if the unidentified foods for sugar, starch, protein and fat (lipid) content. After comparing the color changes in each unknown substance then you will be able to see the amount of sugar, starch, protein or fat there is in the unknown substance.
The state of the American Health in the U.S has become an increasing concern of many Americans. An article entitled “11 Facts about American Eating Habits,” addresses the state of food in the U.S by stating, “Healthiness of the food we eat decreases by 1.7 percent for every hour that passes in the day.” Experts and scientists in the U.S has raised questions about regarding the different ways food is now being produced. While individuals are usually not aware of the ingredients that food contains, many people continue to have unhealthy eating habits. In the past few decades, food production has included numerous artificial ingredients that are said to be the cause of various health problems. Various artificial ingredients that are commonly
In Part C of the experiment, the process completed in Part B was performed again on the six Kool-Aid drink samples: black cherry, cherry limeade, grape, mixed berry, strawberry, and tropical punch. The cherry limeade, grape, mixed berry, and tropical punch samples were undiluted stock solutions that were used in the SpectroVis. The black cherry solution was diluted with a 2-fold dilution. The 3 mL of black cherry solution was diluted with 3 mL of deionized water. This was done because the Red 40 dye was too concentrated. The strawberry solution was first diluted by a 2-fold but the absorbances values were too high and fell outside the acceptable range of 0-1. Therefore, 8 mL of strawberry solution was diluted with 12 mL of deionized water. These values for all of the solutions were recorded in Table 5. The solutions were emptied down the sink. All the glassware and cuvettes were rinsed with deionized water and dried. The volumetric flasks and caps were returned to the appropriate containers.
These results shown from this experiment led us to conclude that enzymes work best at certain pH rates. For this particular enzyme, pH 7 worked best. When compared to high levels of pH, the lower levels worked better. The wrong level of pH can denature enzymes; therefore finding the right level is essential. The independent variable was the amount of pH, and the dependent being the rate of oxygen. The results are reliable as they are reinforced by the fact that enzymes typically work best at neutral pH
First students obtained 8 graduated cylinders and labeled them. Each one contained a different ratio of vinegar and water. These graduated cylinders with the liquid were weighed and recorded.Then, students obtained an Alka-Seltzer tablet and recorded its mass. Then one alka-seltzer tablet was dropped into each of the graduated cylinders. Students had to
Enzymes are proteins which can catalyse chemical reactions without changing themselves. The enzyme lipase breaks down the fat in dairy products such as full-cream milk for people who are lactose intolerant. Lipase acts on its specific substrate, lipids produces fatty acids. If enzyme concentration increases, random collisions between the substrates and active sites of enzyme increase due to the increasing amount of active sites which allow more collisions to happen, so the rate of breakdown of lipids to simpler substances will increase. During the experiment, sodium carbonate solution and pH indicator phenolphthalein will be added ahead of
Analysis: In this experiment, every substance tested positive for at least 1 organic compound. Corn oil tested positive for lipids. Honey and oats tested positive for starch. Honey, apple juice, and unknown 1 tested positive for sugar. Egg white, gelatin, and unknown 2 all tested positive for proteins. So, not a single substance didn't test positive for at least 1 organic compound. All the indicators used in this experiment change color when in the presence of a compound; therefore, they chemically react with the compound. No errors were made during this experiment
Food additives can be found in lots of food that most people consume. Although food additives can make our food look delicious and even taste better, they contain many dangerous chemicals that may affect our health. Food additives can cause health problems in a person’s lifestyle. Many people do not realize the harm it can cause to their bodies. There are many problems, but also solutions to this situation. The solutions to this situation are: stay away from all kinds of additives, do not eat food that are preservatives, read the labels carefully, and find the FDA signs.
Abstract: Enzymes, catalytic proteins that at as catalysis which makes the process of chemical reactions more easily. There are two main factors that actually affects enzymes and their functions which are temperature and pH. Throughout this experiment, the study how pH and peroxidase affects each other and the enzyme was made. The recordings of how the enzymes responded when it was exposed to four different pH levels to come up with an optimum pH which was predicted in the hypothesis and the IRV at the end.
Enzymes are necessary to chemically change and digest molecules in order to be absorbed and used by the body to function. They are catalysts of the body which increase rate of reaction without undergoing a chemical change, and have active sites where the substrate molecules fit. The substrate has a complementary shape that fits with the particular active site and have various uses, such as rennin for example. Rennin is found in the gastric juice of humans and it begins the digestion of milk. In this experiment, junket tablets containing rennin will be added to a sample of milk, curdle it, and replicate the first stage of its