Lab : Differential Staining- Visalization Of Bacterials Cell Strutures Lab

let it stand for 5 minutes. The first test I am going to do will be at

After added, pick up the beaker and swirl it around lightly for a short period of time.

6. The disks in the 0.00% solution were transferred to an agar plate held next to the blue flame using the sterilized tweezers. Excess disinfectant was removed from the disks by wiping on the side of the well of the spotting tile. When the 5 disks were positioned (refer to Figure 1 below) the lid was replaced and sticky taped down. A label was added indicating the concentration of disinfectant.

2. Using the tweezers, lift the steel wool out of the vinegar and shake if gently over a paper towel.

The next step of the project included preparing a Gram stain to discover the cell shape, arrangement, and if the bacteria is gram positive or

Add 3 drops of iodine solution * Shake it well,

5 Remaining in the darkroom move print to tray of running water, do 30-45 second rinse. Squeegee print, place on flat tray.

As soon as the water was added a timer was set for two minutes and the slide was placed under the microscope.

Lab Day 2: The first procedure that was done was a simple stain to identify the bacterial shape. Bacteria tends to be transparent, so a stain must be use to color the cells of the bacteria so it can be viewed under a compound microscope. After heat-fixing three separate loopfuls of my unknown bacteria onto a slide, I used Methylene blue, Safranin, and Crystal violet to stain the three different samples. After rinsing the slide and observing my finding under the microscope, I

To observe mitosis in onion root tip cells and record the different phases of mitosis.

few tablespoons of water, and then cover with a plastic wrap. Let this heat for

Check whether it is ready or not, and then open the burette to start neutralize the acetic acid. (Check the progress by change in colour)

The materials used for the first part of the experiment comprised of the following: a microscope, 4 slides, 4 slide covers, blood samples, lancet, a sheet of paper towel, 3 test tube droppers, Solutions A, Solutions B, and Solution C.

At this stage slides were Dehydrated by placing sections in 70% alcohol (2mins),Then 90% alcohol (2mins) and then Alcohol Eosin (2mins) Again 90% alcohol (30s) and then abulate alcohol (1min), Slides were placed into histoclear (2mins). Small drop of histoamount (4-5mm) were place on a cover slip, slides were removed from histoclear and placed into the cover slip and were ready to be vewied under light

Remove the tubes and add 2-3 drops of Iodine – potassium – iodide solution to each tube.