Purpose: The purpose of this lab was to test for sugars, starches, proteins, and lipids.
Background information:
Biological macromolecules are the large molecules necessary for life. There are four different types of macromolecules which include carbohydrates, lipids, proteins, and nucleic acids. Each macromolecule has a specific function like providing energy or simply insulation. Macromolecules are made from monomers which come together through dehydration synthesis or hydrolysis to create the polymer or macromolecule.
Carbohydrates are made from monosaccharides which are shaped like hexagons and come together to make polysaccharides which provide quick energy, structural support for plants (cellulose in cell walls) and are also found as chitin in the exoskeletons of arthropods. Lipids are made from glycerol and three fatty acids and are found in two different forms-unsaturated and saturated. Lipids provide long term energy storage, insulate and cushion, and prevent water loss. Proteins are made from amino acids which come together forming a protein with peptide bonds. Proteins serve as hormones, muscle, parts of the immune system, and enzymes but if they are the wrong shape, they can 't do their job. Nucleic acids are made from nucleotides and serve as DNA and RNA which store genetic info and transfer info to make new cells.
When conducting this experiment, certain safety precautions must be taken. Proper protective gear must be worn including goggles and closed-toed
They are all polymers made during dehydration synthesis reactions from monomers. These reactions result in the loss of water forming strong covalent bonds. They are referred to as endergonic reactions since they require an input of energy. Hydrolytic reactions are the opposite as they break polymers into single unit monomers using water and in the process releasing energy thus referred to as exergonic reactions (Gorrod, 1985). The monomer form of carbohydrates is a monosaccharide e.g. glucose (used for energy in cells), fructose and ribose. When two monosaccharides undergo a dehydration synthesis reaction they form a disaccharide e.g. sucrose, which is a combination of glucose and fructose. Sucrose is used for transport in plants. Polysaccharides consist of three or more covalently bonded monosaccharides. Starch and glycogen are good examples and are both used in energy storage in plants and animals respectively. Monomers of lipids are fatty acids and glycerol while amino acids are monomers of proteins. Amino acids mainly function to regulate different cell functions as well as catalyze various cell reactions. Nucleotides are the monomers of nucleic acids and they function in storing the genetic information of a cell (DNA) and synthesis of proteins (RNA). All these monomers undergo dehydration synthesis reactions to form their corresponding polymers and are used in
The purpose of this lab is to test for enzyme activity, look at enzyme specificity, and how temperature affects enzyme activity.
The purpose of this lab was to test different substances using various procedures to see what biomolecules were present and ultimately find out what restaurant Anna Lyza had eaten at before she died. For the first control test, we used vegetable oil to test for lipids. So, if the solution does not contain lipids, it does not become translucent when placed onto a paper bag square and held up to a light. So, it is a negative result. However, in the presence of lipids, the solution will become translucent when placed onto a paper bag square and held up to a light. Therefore in this case, the result is positive. On the other hand, we used albumin egg to test for proteins in another control test. If the solution does not contain proteins, it will not experience any color change and so it is a negative result. When there are proteins existing in the solution, it will turn bluish/purplish and for this reason it is a positive result. Furthermore in the third control test, we used dextrose to test for simple carbohydrates such as glucose. If the solution does not contain simple carbohydrates, it will not undergo any color change and will remain a blue color. So, it is a negative result in this circumstance. If there are simple carbohydrates present in the solution, the solution will turn reddish and so the result is positive. For the last control test, we used starch solution to test
The most common macromolecules found in living organisms are lipids, carbohydrates, proteins and nucleic acids. Briefly, the meaning of macromolecules is that they normally contain two or more molecules in them and their main functions are to store energy, information and much more. Most foods are known to be combinations of macromolecules. While some of these compounds can be detected by taste tests, many cannot. Scientists then use certain tests to determine the presence of macromolecules.
Carbohydrates are used for energy and structural support in cell walls of plants and exoskeletons of crustaceans and insects. They are also used to make cell structures and can make a large group of chemical compounds. Carbohydrates are also made up of carbon, hydrogen and oxygen. The testing solution for carbohydrates is Benedict and iodine. Next is proteins, they help bulk up all solid materials inside your body, such as your muscle, hair, skin, and inside organs. Proteins also make up some hormones which help with the chemical control in the body. They
a. Proteins- macromolecules made of amino acids. Proteins have many functions in the body. Some sources of proteins are whole-grain cereals, dark breads,rice, beans, and meat.
7)a) You would do the Benedict’s reagent test for simple sugars and the Lugol’s solution or Iodine test for polysaccharides and starch. To test the lipids you would use the Sudan iV test and Biurets reagent test for protein.
A macromolecule can be any of a general selection of molecules, such as: proteins, carbohydrates, lipids, and
Lipids are molecules that contain hydrocarbons and make up the building blocks of the structure and function of living cells. (Dr. Ananya Mandal, MD, 10/09/2012, Para.1) The lipids that are usually in our foods are fats and oils. Lipids are hydrophobic, meaning they have little or no affinity for water. (Neil A. Campbell,
Then, nucleic acids are made up of nucleotides and has the ability to make new cells. Finally, Lipids. Lipids are made up of glycerol and fatty acids. Lipids play a big role in the body, it provides cushion and insulation for the larger organisms in our bodies. All of these macromolecules and monomers are needed to make energy.
Carbohydrates are sugars and starches and are made of monomers called saccharides, which are connected together to make a polymer called polysaccharides. Carbohydrates give energy to living things, and a special type of carbohydrate called cellulose makes a plant’s cell walls. Proteins are made of monomers called amino acids. Proteins make up the muscles and structural tissue in a body, and they make enzymes which control chemical reactions. Proteins form peptide bonds between amino acids, which make up a polypeptide.
A macromolecule is a large molecule composed of small molecular units called monomers that are linked to together in long chains called polymers. Lipids are hydrophobic, non polar macromolecules that insulate organs, store energy, and make up cell membranes. Their monomers are called triglycerides. Proteins are another example of macromolecules. Made up of monomers called amino acids held together by peptide bonds, proteins provide structural support, defense, and cell regulation. The third macromolecule that is going to be tested for are carbohydrates. Carbohydrates, such as sugars and starches, are needed for energy storage and are made up of monomers called monosaccharides.
The macronutrients (excluding fiber and water) provide structural material (amino acids from which proteins are built, and lipids from which cell membranes and some signaling molecules are built) and energy. Some of the structural material can be used to generate
There are four types of biomolecules, carbohydrates, lipids, proteins, and nucleic acids. Carbohydrates are large chains of sugar found in food and living tissues. This includes sugars, starch, and cellulose. They have the same ratio of hydrogen and oxygen that water has, 2:1. They are broken down to release energy in the animal body. Lipids are any organic compounds that are fatty acids and don’t dissolve in water but do in organic solvents. Fatty acids can be found in natural oils, waxes, and steroids. Proteins are macromolecules that do everything in the cell. They are tools and machines that make things happen. Nucleic acids are long strands of nucleotides, and function primarily in storage and transmission of genetic information. There are two types of nucleic acids, DNA and RNA. DNA is the genetic material of all cellular organisms, and RNA sends out messages from the information that is held in the DNA.
Purpose the purpose of this experiment was to perform test to detect the presence of carbohydrates, proteins, lipids, and nucleic acids. Explain the importance of a positive and a negative control in biochemical test. Use biochemical test to identify an unknown compound.