The Effect Of Anti Microbial Products On Zone Of Inhibition

This experiment was conducted to find the genus and species of an unknown bacteria prescribed by the lab teacher, which was unknown bacteria GA3 in my case. Identification of unknown bacteria techniques are used on an every day basis to figure out what type of bacteria it is and to find the best method of how to treat a patient with this bacteria (1). All five “I’s” of Microbiology were used in the testing for the unknown culture. Inoculation was used several times to put the unknown culture into agar plates or into biochemical test tubes. After Inoculation of these tubes or plates, they always were placed into the incubator for further growth and development. Isolation was used to make sure we got the correct bacteria we were testing for. After each further isolation, we gram stained the culture and inspected the culture under a microscope to further help in the identification process of the unknown bacteria. Multiple tests were done on the unknown culture to make sure we were confident in what kind of bacteria the unknown was.

This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others make antibiotics used today. This lab was completed by using the methods learned thus far in identification of bacteria.

Rubbing alcohol, also known as isopropyl alcohol “refers to two water-soluble chemical compounds—ethyl alcohol and isopropyl alcohol—that have generally underrated germicidal characteristics” (“Guideline for Disinfection and Sterilization in Healthcare Facilities”). Ethyl alcohol kills bacteria in two ways: “protein denaturation and dissolving the lipid membrane” (“How Does Ethyl Alcohol Kill Bacteria?“). Next, Betadine, also known as Povidone-iodine is a solution [that is] often used in hand scrubs and hand rubs in hospital settings” (Ngan). It is used in “Treating minor wounds and infections, as well as killing bacteria” (“Povidone/ iodine solution”). Povidone-iodine’s way of killing bacteria is still being researched and “is yet to be fully understood, [but] it appears to have the ability to penetrate the cell wall of bacteria and affect their structure and function. This ensures their rapid destruction and helps protect against wound and skin infection” (“What is an Antiseptic”). Germ-X Hand Sanitizer is a hand gel that is used for waterless “washing” of hands to kill germs on the surface. It is an antiseptic primarily used by those who want to kill germs quickly and cannot wash their hands (Samuels). The formula contains about 63 percent ethyl alcohol according to livestrong.com (Samuels). Neosporin is a topical antibiotic used to

The disinfectant that was the most effective was the Wescodyne® due to the fact that it was the only disinfectant that had a zone of inhibition for both E. coli and S. aureus, and the zone of inhibition was quite large, with it being 16 millimeters for E. coli and it being 19 millimeters for S. aureus.

The objective of this experiment was to identify two unknown bacteria from a mixed culture. Which was done by using the aseptic technique which was very important to avoid any contamination and keeping the workspace clean while culturing bacteria for different tests. To start, I chose a tube which had a solution of mixed culture. I used the flame to sterilize the inoculating loop and dipped it into the tube and streaked for isolation on 2 TSA plates and placed them in an incubator at 37 for 24 hours. Next day I observed the growth of 2 different types of colonies one for each unknown on the two plates. So I picked the best one and labeled it as master plate and discarded the other plate. From the master plate, I subcultured each type of colony

9. After the 48 hour time period the inhibition of the Escherichia coli bacteria around the disinfectant disks was measured. A ruler was used to measure the clearing from the edge of the disk to the perimeter of the clearing. Irregularities in the clearing were not included in the area of clearing.

“Ajax Spray ‘n’ Wipe” specifically states that it kills E. coli. This is because of the lactic acid in the spray, which is used as a natural antibacterial agent.” ("Colgate-Palmolive | Product Ingredients"). Lactic acid kills unwanted bacteria especially in food which is where E. coli is often found and transmitted, of which meat is the most common cause. Vinegar was chosen as a substance because “it can be used as a bleach alternative” (“Disinfectants: A Guide to Killing Germs the Right Way”) and bleach kills E. coli. It is also used as an everyday cleaning product. The last substance chosen was salty water because it is thought that salt kills bacteria. Much of the world’s population has access to salt and if the experiment proves that salt kills E. coli it would be a helpful factor for further treatment of the

In 1928 Dr. Alexander Fleming began to sort through a pile of petri dishes containing colonies of bacteria that causes boils, sore throats, and abscesses. As he sorted through the dishes he noticed something unusual on one dish. It was dotted with colonies, and one area had a blob of mold growing. The area of mold was later identified as a rare strain of Penicillium notatum, the mold had secreted something that inhibited bacterial growth. Fleming later uncovered that this mold was capable of killing a wide range of harmful bacteria such as streptococcus, meningococcus and the diphtheria bacillus, that had played humanities. Prior to the discovery of Penicillin, Sulfa drugs were used during World War II. These drugs were name as the “wonder drug”. Although they’re still used today, Sulfa drugs were used to cure bacterial infections such as UTIs and gonorrhea. The new found drug penicillin, would cure millions and replace the use of Sulfa drugs.

The antibiotics will have different zones of inhibition on different bacteria. Also, a range of concentrations of the nutrients in the bacteria will change the bacterial growth.

The aim of this investigation was to find out which antiseptics were most effective at preventing the growth of bacteria.

The hypothesis was not supported because rubbing alcohol did not proved to be the household item with the largest zone of inhibition. Starting with the largest zone of inhibition and ending with the smallest, Betadine was the largest, then the Lysol cleanser, liquid hand soap, rubbing alcohol, Neosporin, Germ-X, and the smallest was the distilled water. Betadine had the largest zone of inhibition of 39.5mm and rubbing alcohol which does not have the largest zone of inhibition of 17.7mm, which rejects the hypothesis. Betadine had the largest zone of inhibition because the active ingredient was povidone-iodine. Povidone-iodine kills eukaryotic and prokaryotic cells and is an antiseptic. It penetrates the cell wall quickly and disrupts proteins

The main objective of this experiment is to investigate the effect of different types of antibiotics on bacteria Bacillus subtilis and Escherichia coli. Some of the main methods used in this experiment

My purpose of the experiment is to test what mouthwash will kill the most oral bacteria or prevent the most oral bacteria. What I want to know from this experiment is what kind of mouthwash will prevent the most bacteria and if its wort the money. I choose this topic to see what product will help me maintain the good oral health.

Inhibition of various targets in bacteria cells has long been a strategy for developing new antibiotics. Within a bacteria cell, there are nearly endless potential targets for inhibition that may interrupt cell metabolism, replication, synthesis of membrane components, etc. However, the rise of antibiotic resistance means researchers are continually searching for new targets or ways to make old targets viable again. For this reason, novel narrow-spectrum inhibitors are of great interest, especially since the more species-specific they are, the less potential there is for resistance to spread across bacterial species. However, there is also the hope for the discovery and development of new broad-spectrum inhibitors that could be used to side-step present antibiotic resistance while research on treatments that slow the acceleration of antibiotic resistance continue.

Antiseptics are antimicrobial substances applied to skin, mucous membranes or wounds for their bactericidal or bacteriastatic anti-infective effects (McDonnell and Russell, 1999).