My question is “Does the temperature of a substance effect it’s ph?.” I will be testing this by using ph indicator strips that change color when it comes in contact with an acid or a base and comparing it with a color chart. The substances I will be using are vinegar, orange juice , and lemon juice. These can all be found at a local grocery store. The ph paper and color chart can be found at Walmart. Besides my experiment there have been other experiments that have tested temperature’s effect on ph. They also wanted to know whether temperature affects the ph. I found an experiment online called “Effect of Temperature on the pH of orange juice” done by students from Nease High School. Unlike my project, they only tested Orange Juice. They used 5 different temperatures, and the result was that the temperature had no effect. Another experiment I found online called “ Does storage temperature affect the ph of Orange Juice” by Anna Elizondo also only used Orange Juice. Her results were that the juice was more acidic at a hot temperature. The last experiment i found was on the lines of my experiment, but was more about my hypothesis. It was called “Does Storage Temperature Affect Orange Juice 's Acidity?”. Like the other experiments, this one only used Orange Juice. They used ph paper to measure the acidity at 40 degrees-50 degrees over the span of 2 days. Their conclusion was that the ph was still the same. I will be using cold cups of vinegar, orange juice,
For our science project, we chose to do one that included luminol. Before we started, we found 3 cites, stating,” luminol will preform better in extreme heat than it does in room temperature or ice water.” Also one cite says,” It is used by crime scene investigators to discover spots of blood, even spots that may have been washed.” The last cite writes,” It was concluded that an inverse relationship exists between surface temperature and the reaction rate of the Luminol test.” Our question/ purpose was,” How does temperature affect luminol?” My hypothesis was that I thought that the warm water would affect the luminol more than the cold water.
Step 1: Label the test tubes 1, 2, 3 and 4, where test tube 1 represents the sample placed on the counter at room temperature, test tube 2 represents the tube placed in the refrigerator, test tube 3 represents the tube placed in the freezer, and test tube 4 represents the sample exposed to boiling water. You will expose catalase to each of these four conditions.
Purpose/Hypothesis: The purpose of this experiment is to use both cabbage juice and pH paper to determine the pH of household items. This way, we can tell which products are basic and which one are acidic. If we use cabbage juice as an universal pH indicator by comparing it to pH paper then pH determined by the cabbage juice will be unstable because by using cabbage juice, it can be different depending on how diluted it is.
Extreme pH levels such as pH 2 and pH 12 breaks the hydrogen bonds between the
DeAngelo, Eric, fundamentals of biology laboratory manual, LCCC,2013, Chapter 5 Cell The Effects Environmental Temperature and pH have on the Activity of Porcine Pancreatic Amylase.
Title: The Effect Temperature of Sodium Thiosulfate Has On The Rate of Reaction with Hydrochloric Acid
The Impact of Temperature and pH on the Enzyme Activity of Catechol Oxidase in Solanum Tuberosum Samples
State the optimum pH for sucrase activity and how sucrase activity changes at more acidic and alkaline pH values. The optimum pH for sucrase activity is 6 and decrease with greater acidic and alkalline values.
4. Temperature will be kept consistent at room temperature - All tested will be done in the Biology laboratory with the windows shut to make sure the temperature will not change during the tests. The higher the temperature, the shorter the time will be needed for the chemical reactions to happen and phenolphthalein to turn from pink to colourless.
The principal objective of this study was to determine how temperature affects the activity of
In this experiment, we investigate the change in temperature caused by adding a chemical substance into the water and dissolving it. The results recorded in the table below show that our hypothesis is correct.
By using acid-base titration, we determined the suitability of phenolphthalein and methyl red as acid base indicators. We found that the equivalence point of the titration of hydrochloric acid with sodium hydroxide was not within the ph range of phenolphthalein's color range. The titration of acetic acid with sodium hydroxide resulted in an equivalence point out of the range of methyl red. And the titration of ammonia with hydrochloric acid had an equivalence point that was also out of the range of phenolphthalein.. The methyl red indicator and the phenolphthalein indicator were unsuitable because their pH ranges for their color changes did not cover the equivalence points of the trials in which they were used. However, the
Enzymes are high molecular weight molecules and are proteins in nature. Enzymes work as catalysts in biochemical reactions in living organisms. Enzyme Catecholase is found on in plants, animals as well as fungi and is responsible for the darkening of different fruits. In most cases enzymatic activities are influenced by a number of factors, among them is temperature, PH, enzyme concentration as well as substrate concentration (Silverthorn, 2004). In this experiment enzyme catecholase was used to investigate the effects of PH and enzyme concentration on it rate of reaction. A pH buffer was used to control the PH, potato juice was used as the substrate and water was used as a solvent.
The purpose of this experiment was to test the effects that temperature, pH, and substrate
The purpose of the two experiments was to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli, Pseudomonas fluorescens, Enterococcus faecalis, Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5, 25, 37, 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different species. After one week they were observed for distinguishable changes by the turbidity showing an indication of bacterial growth, or the clarity an indication of no survival.