Unknown Bacterium Identification Report

An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The first procedure that was done was the gram stain. Under a microscope, if the gram stain is purple, the bacterium is gram positive, if the stain is red, it is gram negative. The next test was the fermentation tests for glucose, sucrose and

This experiment was centered on metabolic and biochemical testing procedures. The rationale of performing these tests was to distinguish six different microbes from one another and to compare how their metabolic and biochemical processes differ from species to species to determine the unknown sample.

The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others make antibiotics used today. This lab was completed by using the methods learned thus far in identification of bacteria.

The purpose of this study project was to carefully isolate and identify two unknown bacteria from a mixed culture. The ability to properly evaluate biochemical test results is also necessary for the identification to be successful. The goal was to apply all of the methods and techniques that have been learned in the microbiology laboratory course for the proper identification of unknown bacteria. A certain amount of bacteria that were used throughout the course were possible bacteria that could be found in a mixed culture. The bacteria that were identified in the mixed culture were Staphylococcus Aureus and Kocuria Rhizophila.

The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible

Many tests were completed on the unknown such as gram staining and inspection under microscopes to find whether the bacterium is gram positive or gram negative. Chemical resistance tests were also performed to see if certain chemicals affected the unknown growth or if it didn’t affect the bacteria at all. Each biochemical test

The biochemical tests, the Gram stain, the Bartholomew and Mittwer’s stain, the microscopic and macroscopic observations, and chemical sensitivity tests helped to identify the unknown bacteria as Citrobacter Freundii. Each biochemical test result maintained consistency with the

The purpose of this study was to identify the unknown bacterium using biochemical tests and various methods that had been learned from previous the microbiology laboratory class. Identifying the unknown bacterium was determined by separating and differentiating possible

In the world of microbiology it is vitally important to be able to discern the identities of microorganisms. Not only is it important in a lab setting but as well as in healthcare in general. Properly identify what strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium. Using all the knowledge of procedures and lab techniques identify the unknown and discuss all the tests you performed.

My lab partner and I were given the Unknown Bacteria number four. We preformed multiple tests on it to determine what bacteria it was and have been able to classify it as Proteus vulgaris. Some identifying characteristics of this bacteria are that it is rod-shaped, gram-negative, catalase positive, produces hydrogen sulfide, is nitrate reduction positive, indole positive, urea positive, and is motile. There are multiple reasons that my partner and I believe that unknown bacteria number four is Proteus vulgaris. The tests that we have conducted and the results we have collected have led us to believe or decision is correct.

The best and most accurate way of identifying an unknown microorganism is by sequencing its DNA, but this is very expensive and only used in highly qualified labs. So, the identification of unknown bacteria number 63 was be done by putting the bacteria through numerous laboratory tests. Microorganisms are different among each other by their macroscopic morphology, microscopic morphology, and the unique metabolic processes they use to survive and reproduce. Identifying an unknown microorganism in the laboratory is important because knowledge is gained on the appropriate way to cultivate an organism, how to correctly read the result of a test, and learning about the different characteristics of the bacteria. All of the following tests were done using the best sterile technique and the most new turbid bacterial growth subculture.

In class, we were given the task of identifying an unknown bacterium broth culture. After receiving number 69, I went through several tests to figure out what bacterium I received. First, I created a slide from my broth by putting a small amount of the unknown broth on to a clean slide and letting it dry for ten minutes. After this, I stained the slide by applying four reagents in order; crystal violet, grams iodine, decolorizer and safranin. From the stained slide, I discovered that this bacterium was gram-negative, which would determine the next couple of tests I would do to identify my unknown bacterium. I began by streaking for confluent growth from my broth culture onto a TSA plate. From the TSA plate, I aseptically transferred a loop

An unknown microorganism from three potential microbes—Bacillus megaterium, Escherichia coli, and Enterococcus faecalis—was determined by performing eight biochemical tests. Biochemical tests that were used to differentiate these possible microorganisms from each other were gram staining, motility with a semi-solid agar tube, oxygen requirements, catalase test, phenylalanine deaminase test, eosin methylene blue agar (EMB) and mannitol salt agar (MSA). The unknown microorganism was further clarified through a polymerase chain reaction (PCR) test and basic local alignment search tool (BLAST) database. Based on all the observations of the tests, the unknown bacterium was identified as B. megaterium.

Culturing bacteria on a media has been an important laboratory technique since Robert Koch discovered how to grow bacteria in a petri dish. Bacteria will cultivate successfully on the media if the right nutrients, temperature, humidity, pH and any other specific conditions were provided. The most basic and common medium of growth on the nutrient broth, which contains water, beef extract and peptone. Although most bacteria will grow in this medium, some bacteria are fastidious and require special growth supplements such as blood or sugar. Further in mixed populations where it would be difficult to isolate the minority type of organism in a simple medium. Therefore, media can be divided into different types depending on the chemical composition in order to help isolate selected microorganism’s. The commonly used media are differential, enriched and selective media.

Between the 1st of June and the 28th of June there was a huge outbreak of Escherichia Coli bacteria in Germany that affected 3,091 people. These people were affected with bloody diarrhoea, 47 of which died as a result of the bacteria. It also caused Haemolytic Uremic Syndrome (HUS) in 838 people - leading to kidney failure, which when investigated was proved to be down to the Shiga toxin that was produced by the E. coli strain. HUS is an abnormal premature destruction of the red bloods cells. The damaged red blood cells then can clog up the filtering system which leads to kidney failure.