use the manual assay as the standard reference and trouble shoot each step of the protocol to improve the design of the automated assay. We will also test additional reagents such as protease K, hybridization mix, and wash buffers in order to improve the robustness of the protocol.
Aim-3: To validate the automated mRNA FISH assay of ARv7, ETS gene fusion and PTEN on DeNovo’s JETTA™ microfluidic chip, Jetta 400 sample preparation system and Vanguard image processing system. Demonstrate the entire system capable of automated single cell mRNA FISH on CTCs using blood samples from patients with metastatic prostate cancer.
This objective consists of two tasks as follows: Task 3.1: Patient blood sample collection and processing. We will collect blood samples from metastatic cancer patients before and after treatment. Dr. Clara Hwang, hematologist-oncologist and Dr. Mani Menon, Urologic surgeon, from the Henry Ford Health System will coordinate the efforts to select the patient population for the study. Letter of support from Drs. Menon and Hwang included. We propose to test 50 from African American patients and 50 samples from Caucasian during the study period. Samples prior to and after treatment will be collected for each patient and processed using the instrument pipeline set up for this study. We will correlate the marker status identified from CTC with the primary biopsy or prostatectomy samples using standard IHC, FISH and RNA ISH on FFPE tissues to validate our finding in
This essay is about why lab procedures and practice must be communicated in a lab.
A typical Risk Management plan (RMP) has five steps – Plan, Identify, Assess, Respond, and Monitor. As we discuss the five steps in details, it would be evident that they hold good in every set up in a business – be it IT or non-IT environment.
Oil hypothesis = the water would probably change consistency, probably thicken up and change color
The “Phase 2: Scanning” portion of the lab instructions were very confusing as they give commands to research and test but this portion of the lab has no interconnectivity to the internet. After much wasted time and talking to the lab assistances, it was noted that the students weren’t supposed to test in this environment but it was just information only and bringing up the help file. To offset and further understand this, the student set up a separate lab environment using Virtual Box with a Kali iso. All the main commands were tested with some different variation of options on a number of sites; advancedmissiongroup.com (the students own personal IP address but with no website), largobooks.com, and umuc.edu. Figure 10 demonstrates the
Introduction: The Scientific Method is a universal set of steps that all scientists use while performing a procedure. The Scientific Method contains six steps. The first step is to think of a problem or question. The second step is to formulate a hypothesis about the original question. The hypothesis should be a educated guess on what the answer to the question should be.
Prostate cancer (PCa) is the commonest malignancy tumour in men and is second in cancer related death after lung cancer. PCa is mainly adenocarcinomas originating from the cortex of the gland (D’Elia et al. 2014).
A lab write up can make or break your results in the science world. Structure is very important. The article “Materials & Methods” is basically a step by step how to guide for anyone learning how to write a lab report.
According to Ferrante, Shaw, and Scott (2011), prostate cancer is the most common cancer and second most common cancer death among men in the United States. Early detection permits appropriate and timely management, which can allow clinicians to treat the cancer effectively. When detected at early or regional stage, prostate cancer has a five-year survival rate of about 100%. Prostate-specific antigen (PSA) is the most widely used tumor marker and was approved by the FDA in 1994 as an aid in the early detection of prostate cancer (Duffy, 2011). PSA screening helps detect prostate cancer earlier, at lower clinical stages, and with a lower Gleason score (Cho et al., 2015).
Study Guide to Help with Laboratory Exam 1 Microbiology 1214 A partial study guide only. Not intended to replace the review sheets or the “Know the following” sections on your labs. These slides are not presented in any particular order.
Table 3 The Averages Absorbance of The Cell Lysate & Cell Pellet In E.coli Induction Experiment
Long non-coding RNAs (lncRNAs) are a class of RNAs that are more than 200 nucleotides long (2) and do not encode proteins. LncRNAs regulate a spectrum of biological functions and their misregulation or differential expression contribute to tumerogenesis. Exploiting this feature of lncRNAs make them excellent candidates for cancer therapy. An important indication of cancer is metabolic reprogramming which is indicated by increase in various pathways in cells such as glycolysis, glutaminolysis, Pentose Phosphate Pathway, synthesis of nucleotide and fatty acids, and formation of citrate through TCA cycle (4). Prostate Cancer Gene expression marker 1 (PCGEM1) (3), is a lncRNA that control the reprogramming of metabolic pathways in prostate
Novel serum markers are vital, in the case of malignancies such as NSCLC, not only for diagnosis but also for prognosis evaluation and follow-up of the patients after treatment(13).
Although there are many methods that help with diagnosing prostate cancer such as biomarkers and needle core biopsy, it is the practice patterns of different pathologists that in the end determine the diagnosis. This article explains how and why interobserver variability can affect the ability to diagnose prostate cancer. Additionally, it determines the outliers for immunohistochemistry work up. Immunohistochemistry is a method that can help aid the diagnosis of prostate needle core biopsy specimens. This procedure looks at the small foci of prostatic adenocarcinomas and limits the over diagnosis of non-neoplastic look-alikes.
Around 8 million people die of cancer every year, whether it’s breast cancer, lung cancer, kidney cancer, or prostate cancer (National Cancer Institute). there is a blood test that will help decrease that number. Now there is a solution that will make that number go down. This new solution is called a liquid biopsy, which is a non-invasive blood test. This test should help make the number of cancer patients go down, the way biopsies are done, and the time we find cancer cells in the blood.
The use of biomarker in clinical practice is pivotal and offers great promise to the physician and surgeon to treat the cancer patients with better management plan. With the use of a biomarker, it will ultimately enhance the treatment response and improves patient’s survival and quality of life. The application of biomarkers in practices of medicine however carries several advantageous and disadvantageous.