. A geneticist examined the amino acid sequence of aparticular protein in a variety of E. coli mutants. Theamino acid in position 40 in the normal enzyme isglycine. The following table shows the substitutionsthe geneticist found at amino acid position 40 in sixmutant forms of the enzyme.mutant 1 cysteinemutant 2 valinemutant 3 serinemutant 4 aspartic acidmutant 5 argininemutant 6 alanineDetermine the nature of the base substitution thatmust have occurred in the DNA in each case. Whichof these mutants would be capable of recombinationwith mutant 1 to form a wild-type gene?
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
. A geneticist examined the amino acid sequence of a
particular protein in a variety of E. coli mutants. The
amino acid in position 40 in the normal enzyme is
glycine. The following table shows the substitutions
the geneticist found at amino acid position 40 in six
mutant forms of the enzyme.
mutant 1 cysteine
mutant 2 valine
mutant 3 serine
mutant 4 aspartic acid
mutant 5 arginine
mutant 6 alanine
Determine the nature of the base substitution that
must have occurred in the DNA in each case. Which
of these mutants would be capable of recombination
with mutant 1 to form a wild-type gene?
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