. Cells containing missense mutations in the crp gene(encoding the positive regulator CRP) are Lac−, Mal−,Gal−, etc. To find cells with suppressors of the crpmutation (that is, cells with the crp mutation thatbehave as if they are crp+), cells were screened tofind those that were both Lac+ and Mal+.a. What types of suppressor mutations would youexpect to obtain using this screen compared with ascreen for Lac+ only?b. All suppressors isolated were mutant in the genefor the α-subunit of RNA polymerase. What hypothesis could you propose based on this analysis?

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A: Signal transduction is a process in which signals from the extracellular level enter the…

Q: You have isolated a protein that binds to DNA in theregion upstream of the promoter sequence of the…

A: Positive regulation refers to the increased transcription of a gene by binding of a protein or a…

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Q: Compare and contrast the regulation of Ras with trimeric G proteins

A: Given: Need to compare and contrast the difference between Ras protein with trimeric G protein.

Q: After the initial Actualization of the Cit+ phenotype, there was another alteration to the A-3…

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Q: Cancer-promoting mutations are likely to have different effects on the activity of proteins encoded…

A: Cancer is the unnatural and excessive proliferation of cells that becomes harmful to normal body…

Q: Using CREB and nuclear receptors as examples,compare and contrast the structural changes that…

A: Transcription factors are defined as the protein responsible for controlling the transcription rate…

Q: Mutations that inactivate p53 have a recessive phenotype, whereas mutations affecting Ras are…

A: P53 is a tumor protein, which codes for the protein that regulates the cell cycle and suppresses…

Q: in a fat cell what is the result of activation of genes by PPAR gamma/activator proteins binding?…

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Q: What is/are product(s) from commensal bacteria that regulate the expression for ler, regulator of…

A:

Q: Name the mutation at the protein level which is the basis for mutant A-1 Would a nonsense mutation…

A: 3) Name the mutation at the phenotype level when a new function is acquired: _Nemo morphic     nemo…

Q: (a) Did deletion of any of the possible control elements cause anincrease in reporter gene…

A: Insertion and deletion are naturally processes occurring within the DNA or mRNA sequence for…

Q: Describe the ATM-p53 repair pathway in normal cells and cancerous cells

A: Apoptosis is a genetically controlled mechanism of cell death, which is important for the…

Q: The figure below shows 6 genes that are differentially expressed based on their specific enhancers.…

A: Enhancers are the regulatory sequences in the DNA located farther away from the transcription start…

Q: IPTG. Explain the mechanistic steps whether the expression of beta-galactosidase (Z) and permease…

A: I) In case of the first merodiploid the constitutive mutation of the operator does not allow the…

Q: What is the effect of supper repressor (lacI^s) mutations?

A: Lac operon is a transcriptionally regulated system in E.coli which enables the bacteria to utilize…

Q: The use of an SH2 domain binding inhibitor would be predicted to: O Decrease binding of SOS with RAS…

A: In the cellular response, the SRC homology (SH2) 2 domain is considered as the protein, which is…

Q: An enhancer, located upstream from a gene, has the followingsequence:   5´–GTAG–3´   3´–CATC–5´This…

A: Deoxyribonucleic acid (DNA) is a genetic material and it carries genetic information from one cell…

Q: Explain in detail the role of super-enhancers. Give some examples. How the super-enhancers can be…

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Q: In an E. coli mutant, beta galactosidase and permease are expressed at high levels when both glucose…

A: In normal condition, when lactose level is high and glucose level is low, galactosidase and permease…

Q: A -------| B-------> Violet A linear switch-regulation pathway of the type shown here controls…

A: The products of genes A and B are involved in a pathway responsible for flower color formation. The…

Q: Briefly describe how the cyclin D-cdk4/6 and cyclin E-cdk2 complexes regulate Retinoblastoma protein…

A: Retinoblastoma is the disease occurring in the retina of the eyes. This is basically eye cancer…

Q: Indicate whether the protein binds directly or indirectly (assuming it does) to DNA and whether to…

A: Introduction :- DNA acts the genetic material of the cell because it have all the genetic…

Q: 8. Yeast genes have cis-acting elements upstream oftheir promoters, similar to enhancers, called…

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Q: Distant regulatory sites are called

A: During gene transcription some  regulatory genes are attached to the transcription site which…

Q: Based on the labels you should be able to recognize the characteristic structure depicted below and…

A: Introduction The Process Of Turning Nucleic Acid Information Into Amino Acids Is Known As…

Q: We have studied Ras signal transduction pathway. In an experiment,  two different mutations were…

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Q: [PTG. Explain the mechanistic steps whether the expression of beta-galactosidase (Z) and permease…

A: In case of ii) or second merodiploid the mutation in the repressor in the chromosomal DNA ( the part…

Q: a. How do you think the PLAT gene expression is specifically regulated only in the endothelial…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: How does the activity of human p53 get regulated through the interaction of the transactivation…

A: p53 is basically a transcription factor that helps in suppressing the growth of the tumor. This…

Q: Discuss the following argument: “if the expression of every gene depends on a set of transcription…

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Q: ''In the cellular regulatory pathways that controlcell growth and proliferation, the products of…

A: Cell regulation involves a complex mechanism of different cell signaling pathways that work in a…

Q: A mutation in the Ras protein renders Ras constitutively active (RasD). What is constitutive…

A: The Ras protein can be referred to as tiny G protein that requires phosphorylation of guanosine…

Q: The binding of a small effector molecule, protein-protein interactions, and covalent modifications…

A: Transcription factors that bind to regulatory elements are called as regulatory transcription…

Q: A translocation mutation results in a silencer being inserted just upstream of the promoter of a…

A: When a repressor protein binds to the silencer region of DNA,  polymerase is prevented from…

Q: Many genes whose expression is turned on by DNAdamage have been isolated. Loss-of-function mutations…

A: The lex A gene encodes a repressor protein and is involved in controlling the DNA repair, inhibition…

Q: ame the mutation at the protein level which is the basis for mutant B-1 Would a nonsense mutation…

A: "IT IS NOT MENTIONED , WHICH QUESTION TO ANSWER , SO ONLY FIRST ONE IS ANSWERED". It is well known…

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Q: Tumor cells from a person with leukemia have been analyzed to determine which oncogene is involved…

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Q: For each of the ff. scenario, state whether the gene is up- or down-regulated and briefly explain…

A: Up-regulation of gene expression refers to the increased expression of a gene by increasing the…

Q: When challenged with a low oxygen environment, knownas hypoxia, the body produces a hormone called…

A: DNA (deoxyribonucleic acid) is wrapped around proteins (known as histones) to form a structure known…

Q: Relative to each promoter, where would you predict phosphorylated OmpR would bind the ompC and ompF…

A: EnvZ/OmpR are a part of a two-component regulatory system extensively found in bacteria and it has…

Q: Describe which enzymes are required for lactose and tryptophan metabolism in bacteria when lactose…

A: Tryptophan is aa that is required for proper newborn growth as well as the formation and maintenance…

Q: The UG4 gene is expressed in the stem and leaf tissue of the plant Arabidopsis thaliana. To identify…

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Q: If you were given a tube containing only a purified protein of interest but had unlimited time and…

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Q: While investigating the function of a specific growth factor receptor gene from humans, researchers…

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Q: Expression of a gene, in terms of greater production of the protein it encodes, could be increased…

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Q: The RB (retinoblastoma) protein normally                             [ Select]     ["keep a…

A: Retinoblastoma is a disease occurring in the retina of the eyes. This is basically eye cancer…

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.