Plasmin is an essential enzyme for clot breakdown (hydrolysis of fibrin protein toFibrinogen degradation product (FDP)) as shown in the figure below.tPA is a serine protease that catalyzes the hydrolysis of plasminogen to plasmin.Cardiovascular disorders such, as heart attack and stroke are among the top ten causes ofdeath. Stroke patients, under certain circumstances, have better outcomes when treatedwith tissue plasminogen activator (tPA).In humans, the PLAT gene codes the tPA protein, and is expressed only in endothelialcells that line the blood vessels.plasmin → fibrinolysisFDPtPAfibrinplasminogena. How do you think the PLAT gene expression is specifically regulated only in theendothelial cells? Provide two specific explanations. Explain briefly.b. Considering the therapeutic potential of tPA, you and your friend want to produce alarge amount of human tPA using recombinant technologies. You suggest cloning theintact human PLAT gene in bacteria to express it and isolate the PLAT protein.Your friend suggests that you instead clone the PLAT CDNA in bacteria.Provide two specific explanations for each suggestion & justify which one is better.Explain briefly why.

Since you have asked multiple questions, we will solve the first question for you. If you want any…

Answered: a. How do you think the PLAT gene…

a. How do you think the PLAT gene expression is specifically regulated only in the endothelial cells? Provide two specific explanations. Explain briefly.

Human Physiology: From Cells to Systems (MindTap Course List)

9th Edition

ISBN:9781285866932

Author:Lauralee Sherwood

Publisher:Lauralee Sherwood

Chapter11: The Blood

Section: Chapter Questions

Problem 3SQE

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Concept explainers

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.

Question

Plasmin is an essential enzyme for clot breakdown (hydrolysis of fibrin protein to
Fibrinogen degradation product (FDP)) as shown in the figure below.
tPA is a serine protease that catalyzes the hydrolysis of plasminogen to plasmin.
Cardiovascular disorders such, as heart attack and stroke are among the top ten causes of
death. Stroke patients, under certain circumstances, have better outcomes when treated
with tissue plasminogen activator (tPA).
In humans, the PLAT gene codes the tPA protein, and is expressed only in endothelial
cells that line the blood vessels.
plasmin → fibrinolysis
FDP
tPA
fibrin
plasminogen
a. How do you think the PLAT gene expression is specifically regulated only in the
endothelial cells? Provide two specific explanations. Explain briefly.
b. Considering the therapeutic potential of tPA, you and your friend want to produce a
large amount of human tPA using recombinant technologies. You suggest cloning the
intact human PLAT gene in bacteria to express it and isolate the PLAT protein.
Your friend suggests that you instead clone the PLAT CDNA in bacteria.
Provide two specific explanations for each suggestion & justify which one is better.
Explain briefly why.

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