Auxotrophic mutation 103 grows on minimal medium supplemented with A, B, or C; mutation 106 grows on medium supplemented with A or C, but not B; and mutation 102 grows only on medium supplemented with C. What is the order of A, B, and C in a biochemical pathway?

Auxotrophic mutation 103 grows on minimal medium supplemented with A, B, or C; mutation 106 grows on medium supplemented with A or C, but not B; and mutation 102 grows only on medium supplemented with C. What is the order of A, B, and C in a biochemical pathway?

Name: Auxotrophic mutation 103 grows on minimal medium supplemented with A,
Uploaded: 2024-03-20T06:42:55.000Z
Channel: Bartleby
Description: Auxotrophic mutation 103 grows on minimal medium supplemented with A, B, or C; mutation 106 grows on medium supplemented with A or C, but not B; and mutation 102 grows only on medium supplemented with C. What is the order of A, B, and C in a biochemi

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter15: From Dna To Protein

Section15.1: The Connection Between Dna, Rna, And Protein

Problem 1SB

See similar textbooks

Similar questions

A mutant has no activity for the enzyme isocitrate lyase.Does this result prove that the mutation is in the geneencoding isocitrate lyase?
Various kinds of biochemical events may explain why some disease alleles are dominant?
When Avery and his colleagues had obtained what was concluded to be the transforming factor from the IIIS virulent cells, they treated the fraction with proteases, RNase, and DNase, followed in each case by the assay for retention or loss of transforming ability. What were the purpose and results of these experiments? What conclusions were drawn?
In the experiments of Aery, MacLeod, and McCarty, what was the purpose of treating the transforming extract with enzymes?
Let’s suppose you make a transposon library of the cellulose-secreting bacterium Komagataeibacter xylinus, with the goal of finding mutants that produce higher than normal amounts of cellulose, which would be useful industrially. However, despite your best efforts you are unable to isolate any transposon mutants that make more cellulose than the wild-type strain.Why might this have failed? List as many reasons as you can think of.
What are the two main mechanisms by which cells repair doublestrandbreaks? Briefly describe each one.
What are biochemical characterisation of transforming principle?
Why are the correct primer sequences essential for successful amplification?
In preparation for transformation, why do you think we used calcium chloride and magnesium chloride in making the cells competent? Would your results be different if monovalent ions were used instead of divalent ions? Why do you think so?
In a cotransformation experiment DNA was isolated from a donor strain that was proA+ and strC+ and sensitive to tetracycline. (The proA and strC genes confer the ability to synthesize proline and confer streptomycin resistance, respectively.) A recipient strain is proA− and strC− and isresistant to tetracycline. After transformation, the bacteria werefirst streaked on a medium containing proline, streptomycin, andtetracycline. Colonies were then restreaked on a medium containingstreptomycin and tetracycline. (Note: Each type of medium hadcarbon and nitrogen sources for growth.) The following resultswere obtained:70 colonies grew on the medium containing proline, streptomycin,and tetracycline, but only 2 of these 70 colonies grew whenrestreaked on the medium containing streptomycin and tetracyclinebut lacking proline.What would you expect the cotransformation frequency to be ifthe average size of the DNA fragments was 4 minutes and thetwo genes were 1.4 minutes apart?
In this problem, just put the order of the intermediates on the pathway, starting with P and ending with Z and explain your reasoning. (So to get you started, notice the class 1 mutants—and there is only mutant in this class, which is mutant #5—won’t grow on minimal medium, but will grow if you give it substance Z. However, giving it substances, p, w, x, or y won’t help. This means that the gene that is mutated lies on the pathway in a place that the Z substances is to its right and all the other substances are to its left. The easiest next one to look at is the line with 2 pluses, and then the line with 3 pluses, and then the line with 4 pluses).
What is the purpose of Southern's blotting technique? Explain in detail the biochemical principle that underpin each step of the method.