1. A reaction in which a substrate is converted to a product is catalyzed by an enzyme. The reaction wasdetermined to have ket = 10,000. s', KM = 90.0 µM, and a standard Gibbs free energy change of -13.8 kJmol. Answer the following questions about experiments performed with 2.00 nM enzyme. R= 8.3145J mol K.!3!a) Calculate the change in free energy for this reaction at room temperature (25.0°C) under thefollowing conditions, and indicate whether the forward reaction is favored or not.i. [(S] = 2.00 µM and [P] = 2.00 nM.!!it. [S] = 2.00 uM and (P) = 2.00 uM.

Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…

Answered: 1. A reaction in which a substrate is…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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For the following reaction X + YA + B at 300 K, it is found equilibrium constant equal to 10. Therefore, AG & AGⓇ of the reaction at 300 K respectively are - Answer b
An enzyme that follows simple Michaelis–Menten kinetics has an initial reaction velocity of 10 µmol⋅min-1 when the substrate concentration is five times greater than the KM. What is the Vmax of this enzyme in µmol⋅min−1?
Assume you have an enzyme that catalyzes a reaction that breaks down dopachrome. At t = 0 s, the absorbance at 475 nm is 0.2 when you add the enzyme. At t = 30 s, would you expect the absorbance to be less than or greater than 0.2?
The equilibrium constant for the reaction Q → R is 25. Solve, (a) If 50 μM of Q is mixed with 50 μM of R, which way will the reaction proceed to generate more Q or more R? (b) Calculate the equilibrium concentrations of Q and R.
An enzyme catalyzes a reaction at a velocity of 20 μmol/min when the concentration of substrate (S)is 0.01 M. The Km for this substrate is 1 × 10-5 M. Assuming that Michaelis-Menten kinetics arefollowed, what will the reaction velocity be when the concentration of S is 1 ×10-6 M?
Suppose you were running out of substrate (PNPP) and decided to reduce the concentration used in your reactions (25 µM, 50 µM, 100 µM, 200 µM, 300 µM) by a factor of 100. How might this affect your kinetic analysis?
The following were obtained in a study of an enzyme known to follow Michaelis-Menten kinetics: Reaction Velocity (mmol/min) Substrate added (mmol/L) 217 0.8 325 2 433 4 488 6 647 20 652 1000 The Km for this enzyme is approximately _____________. (Round to the nearest integer)
If Vmax for a reaction is 10 μM ⋅ s-1 and the KM is 0.5 μM, what is the reaction velocity when the substrate concentration is 2 μM?
A certain first order reaction has the rate law Rate = k[A] with k=0.0068 sec-1. If the initial concentration of A is 0.75 M, what will be the concentration of A after 1 minute? What is the half-life for this reaction? How much time will it take for 75% of A to react? How much A will be left after the passage of three half-lives? What is the initial rate of the reaction?
If we alter the kinetics of a reaction by increasing Km but leaving Vmax alone, how will the L-B plot change?
Given the following set of kinetic data, what is the KM of the enzyme? Numerical answer, only (assuming the same units as seen in the table). [S] (micro M) vo (Micro mol/sec) 1 49 2 96 8 249 50 498 100 656 1000 778 5000 999
2. The following question tests your lab skills as well as basic knowledge understanding: In Lab1- KHP Determination, 0.85 g moisturized NaOH (M.W.= 40.01 g/mol) solid was dissolved in 500.0 mL of water. 24.21 mL of this NaOH solution was consumed to reach the equivalence point when titrated with 0.2021 g of pure KHP (M.W.=204.22 g/mol. Solid was fully dissolved in 50 mL deionized water). (a). Please write the reaction equation (balanced). Then calculate the precise concentration of NaOH solution. List all steps. (b). What are the primary standard and secondary standard material in the above operation respectively? (c). During the above operation, what glassware/tools were used to measure 0.85 g NaOH, to store 1 liter NaOH solution, to measure pure KHP solid, to conduct the titration, respective ? (d). Can this titration be used to measure the pH value of an unknown solution? Why or why not? (e). Did we use a pH buffer solution in the…

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