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The kinetics of an enzyme have been investigated experimentally. Some of the results are given in the figure below in the form of a Lineweaver-Burk plot (sometimes called a double reciprocal plot). In this experiment, the enzyme concentration was 11.5 nM (i.e. 1.15 x 10-8 M). 1/V (s/mM) 2500 2000 1500 1000 500 0 o o o ODO 0 100 200 1/[S] mM-1 300 400

The kinetics of an enzyme have been investigated experimentally. Some of the results are given in the figure below in the form of a Lineweaver-Burk plot (sometimes called a double reciprocal plot). In this experiment, the enzyme concentration was 11.5 nM (i.e. 1.15 x 10-8 M). 1/V (s/mM) 2500 2000 1500 1000 500 0 o o o ODO 0 100 200 1/[S] mM-1 300 400

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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THIS IS SECOND TIME IM POSTING THIS QUESTION. Two experts have given me different results, which have made me more confused than I was to start with.  And please DONT USE any computer program, cause I'm not allowed to use computer during exam. Thank you. 

Details so I can understand, thank you. 

The kinetics of an enzyme have been investigated experimentally. Some of the results are given in the figure below in the form of a Lineweaver-Burk plot (sometimes called a double reciprocal plot). In this experiment, the enzyme concentration was 11.5 nM (i.e. 1.15 x 10-8 M). 1/V₂ (s/mM) 2500 2000 1500 1000 500 0 Omo o o o 0 O 100 200 1/[S] mM-1 300 400
Transcribed Image Text:The kinetics of an enzyme have been investigated experimentally. Some of the results are given in the figure below in the form of a Lineweaver-Burk plot (sometimes called a double reciprocal plot). In this experiment, the enzyme concentration was 11.5 nM (i.e. 1.15 x 10-8 M). 1/V₂ (s/mM) 2500 2000 1500 1000 500 0 Omo o o o 0 O 100 200 1/[S] mM-1 300 400
A: State the Lineweaver Burke equation. B: Calculate the kinetic parameters Vmax and KM from the figure. C: Estimate the turnover number of the enzyme.
Transcribed Image Text:A: State the Lineweaver Burke equation. B: Calculate the kinetic parameters Vmax and KM from the figure. C: Estimate the turnover number of the enzyme.
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