1. Acetate buffers are used in biochemical studies of enzymes and other chemical components of cells toprevent pH changes that might change the biochemical activity of these compounds.(Ka 1.8 x 105)a) Calculate the pH of an acetate buffer that is a mixture with 0.20M acetic acid and 0.20M sodiumacetateb) Calculate the pH after 2.0 mL of 0.10M NaOH is added to 100.0mL of this buffer, giving a solution witha volume 101.0m L

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Asked Oct 30, 2019
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1. Acetate buffers are used in biochemical studies of enzymes and other chemical components of cells to
prevent pH changes that might change the biochemical activity of these compounds.
(Ka 1.8 x 105)
a) Calculate the pH of an acetate buffer that is a mixture with 0.20M acetic acid and 0.20M sodium
acetate
b) Calculate the pH after 2.0 mL of 0.10M NaOH is added to 100.0mL of this buffer, giving a solution with
a volume 101.0m L
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1. Acetate buffers are used in biochemical studies of enzymes and other chemical components of cells to prevent pH changes that might change the biochemical activity of these compounds. (Ka 1.8 x 105) a) Calculate the pH of an acetate buffer that is a mixture with 0.20M acetic acid and 0.20M sodium acetate b) Calculate the pH after 2.0 mL of 0.10M NaOH is added to 100.0mL of this buffer, giving a solution with a volume 101.0m L

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Expert Answer

Step 1

Given

Ka= 1.8 x 10-5

pKa= 4.745

concentration of acetic acid= 0.20M

concentration of sodium acetate= 0.20M

Step 2

a) According to Henderson’s equation

[acid]
pH pK-log
[salt]
[0.20]
4.745-log
[0.20]
=4.745-0
=4.745
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[acid] pH pK-log [salt] [0.20] 4.745-log [0.20] =4.745-0 =4.745

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Step 3

b) When 0.2 ml of 0.1M NaOH is added to the 100ml of solution giving...

2.0
no of moles of NaOH= 0.1Mx-
L
1000
-0.0002
0.0002
molarity
101.0
1000
-0.0019M
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2.0 no of moles of NaOH= 0.1Mx- L 1000 -0.0002 0.0002 molarity 101.0 1000 -0.0019M

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