1. Reaction rate data of an enzyme, E, and a substrate, S, with increasing inhibitor concentrations are listed in the table below (Cs and C₁ represents the concentrations of the substrate and the inhibitor, respectively). Reaction Rates (μmol.Li¹.s¹) [CS] (mmol/L) [CI]=0 [CI]=0.25 [CI]=0.50 mmol/L mmol/L mmol/L 0.10 0.79 0.57 0.45 0.15 1.11 0.84 0.66 0.20 1.45 1.06 0.86 0.30 2.00 1.52 1.22 (a) Calculate Vmax (mmol.m³.s-¹). (b) Calculate Kм (g.mol.m³). (c) Describe the type of this inhibition.

Fundamentals Of Analytical Chemistry
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Chapter30: Kinetic Methods Of Analysis
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Problem 30.15QAP
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1. Reaction rate data of an enzyme, E, and a substrate, S, with increasing inhibitor concentrations are
listed in the table below (Cs and C₁ represents the concentrations of the substrate and the inhibitor,
respectively).
Reaction Rates (μmol.Li¹.s¹)
[CS]
(mmol/L)
[CI]=0
[CI]=0.25
[CI]=0.50
mmol/L
mmol/L
mmol/L
0.10
0.79
0.57
0.45
0.15
1.11
0.84
0.66
0.20
1.45
1.06
0.86
0.30
2.00
1.52
1.22
(a) Calculate Vmax (mmol.m³.s-¹).
(b) Calculate Kм (g.mol.m³).
(c) Describe the type of this inhibition.
Transcribed Image Text:1. Reaction rate data of an enzyme, E, and a substrate, S, with increasing inhibitor concentrations are listed in the table below (Cs and C₁ represents the concentrations of the substrate and the inhibitor, respectively). Reaction Rates (μmol.Li¹.s¹) [CS] (mmol/L) [CI]=0 [CI]=0.25 [CI]=0.50 mmol/L mmol/L mmol/L 0.10 0.79 0.57 0.45 0.15 1.11 0.84 0.66 0.20 1.45 1.06 0.86 0.30 2.00 1.52 1.22 (a) Calculate Vmax (mmol.m³.s-¹). (b) Calculate Kм (g.mol.m³). (c) Describe the type of this inhibition.
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