1. What kinds of media would be used to culture and Identify this microbe?2. What are some other potential microbes that could have this infection? CASE STUDY Part 2MicrorietyA Gram stain is one of the key tests for gettingquick feedback on the kind of microbes that mightbe present in a sample. It is routine in meningitisbecause it can differentiate among several types of bacteriaand detect certain other infectious agents, but it will notdetect viruses. Usually within moments a lab technician cantell if there are bacterial cells and can observe their Gramreaction and shape.Lab results from the Gram stain and culture were conclu-sive: Kay Peterson was infected by the meningococcus, Neis-seria meningitidis,* which is an agent of both meningitis* andsepticemia.* The microscopic examination yielded the classicappearance of tiny pairs of red cocci (diplococci) and whiteblood cells carrying the same cocci inside. A blood culturealso showed growth, indicating that the bacteria had enteredher bloodstream. Plates of agar inoculated with the CSF grewtypical off-white, smooth isolated colonies that tested out asN. meningitidis. Kay remained on the antibiotic regimen for10 days and was released, fortunately without long-termdamage.What kinds of media would be used to culture and identifythis microbe?What are some other potential microbes that could havecaused this infection?For more information on the nature of this agent and itsdisease, see chapter 18 and log on to www.cdc.gov/meningitis/index.html.To conclude this Case Study, go to Connect.ndivi

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Chapter7: Preventing Perioperative Disease Transmission

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