1. Why do we pass the smear in the open flame of the bunsen burner 3 times? What do we want to achieve by doing this? 2. Is the size of the bacterial smear on the slide very critical to the analysis? Why or why not? 3. Is the thickness of the bacterial smear on the slide very critical to the analysis? Why or why not? 4. Why do we pass the smear in a gentle stream of water after flooding it with a stain? What do we want to prevent by doing this? 5. Is the duration or time of stain application very critical in simple staining?

1. Why do we pass the smear in the open flame of the bunsen burner 3 times? What do we want to achieve by doing this? 2. Is the size of the bacterial smear on the slide very critical to the analysis? Why or why not? 3. Is the thickness of the bacterial smear on the slide very critical to the analysis? Why or why not? 4. Why do we pass the smear in a gentle stream of water after flooding it with a stain? What do we want to prevent by doing this? 5. Is the duration or time of stain application very critical in simple staining?

Principles Of Radiographic Imaging: An Art And A Science

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter11: Filtration

Section: Chapter Questions

Problem 5RQ

See similar textbooks

Similar questions

Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can confirm whether a bacterial smear has been correctly prepared or not. Why should you be careful not to underheat a smear during the heat-fixing process? Why do you think the presence of grease or dirt on a glass slide will result in a poor smear preparation? Cite two or three reasons.
What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).
What advantage does the oil immersion objective have over the low power objective when doing bacterial examination?
Complete the following questions for your gram positive coccus unknown organism (a) What test did you run for your second test on your gram positive coccus organism? MSA Test (b) What was the result of this second test? Positive (c) What does the result of your second test tell you about the biological and/or metabolic characteristics of your gram positive coccus unknown microorganism? Please answer (d) If your organism requires a third test, what did you do for your third test on your gram positive coccus unknown organism and what was the result of this test? If it does not require a third test, just put "Gram positive coccus identified". Please answer
In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?
3.1 You can perform a specific staining method to confirm the purity on the culture. Describe this staining method. 3.2 Describe the results obtained from 3.1 and comment about the purity of the culture. 3.3 What staining method would you use to verify that the isolate is not producing survivalstructures against unfavourable conditions? Describe the procedure as well.
(a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside from bacteria, can gram staining be used with microbes such as fungi? Explain your answer.
Assume that, during the performance of this exercise, you madeseveral errors in your spore-staining procedure. In each of thefollowing cases, indicate how your microscopic observations would differfrom those observed when the slides were prepared correctly.a. You used acid-alcohol as the decolorizing agent.b. You used safranin as the primary stain and malachite green as thecounterstain.Experiment 11: lab report 105c. You did not apply heat during the application of the primary stain
You saw no color change in both the first step and second step of the Nitrate Reduction test. What is the result of this experiment? Also, explain what happens when an excessive amount of zinc is added in the second step of this test.
What are the processes of concentrate samples analysis in Uv-visible spectroscopy? Please shortly answer at your own words(3-4 lines).
What will pose an ethical issue in these procedures are performed - sterilization?
Complete the following questions for your gram negative rod unknown organism (a) What test did you run for your second test on your gram negative rod organism? Citrate test (b) What was the result of this second test? Positive (c) What does the result of your second test tell you about the biological and/or metabolic characteristics of your gram negative rod unknown microorganism? Please answer (d) If your organism requires a third test, what do you propose as your third test on your gram negative rod unknown organism? If it does not require a third test, just put "Gram negative rod identified". Please answer