10. Applying the Michaelis-Menten Equation II An enzyme catalyzes the reaction M N. The enzyme is present at a concentration of 1 nM, and the Vmax is 2 µM s. The Km for substrate M is 4 µM. (a) Calculate kcat: (b) What values of Vmax and Km would be observed in the presence of sufficient amounts of an uncompetitive inhibitor to generate an a' of 2.0?

Question
10. Applying the Michaelis-Menten Equation II An enzyme catalyzes the reaction M
N. The enzyme is present at a concentration of 1 nM, and the Vmax is 2 µM s. The Km for
substrate M is 4 µM. (a) Calculate kcat: (b) What values of Vmax and Km would be observed
in the presence of sufficient amounts of an uncompetitive inhibitor to generate an a' of 2.0?
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Transcribed Image Text

10. Applying the Michaelis-Menten Equation II An enzyme catalyzes the reaction M N. The enzyme is present at a concentration of 1 nM, and the Vmax is 2 µM s. The Km for substrate M is 4 µM. (a) Calculate kcat: (b) What values of Vmax and Km would be observed in the presence of sufficient amounts of an uncompetitive inhibitor to generate an a' of 2.0?