11. You isolated a gene expressed in differentiated hepatocytes in mice. You then fused various fragments of the gene (shown as dark lines in the following figure) to a lacZ reporter lacking either enhancers or a promoter. The resulting clones were introduced into hepatocytes in tissue culture, and the level of lacZ expression was monitored by using a colorimetric assay. From the results that follow, which region contains a likely hepatocyte enhancer? a. a b. b с. с d. d e. e lacz expression 100 gooonnons 5 100 5 5 Fragments fused to lacz lacz A d e ✓ abc E-DO promon

Biology: The Unity and Diversity of Life (MindTap Course List)
14th Edition
ISBN:9781305073951
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Chapter31: Animal Tissues And Organ Systems
Section: Chapter Questions
Problem 1CT: IPSCs are nearly identical to human embryonic stem cells in terms of gene expression, but there may...
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please give thorough explanation for why answer is B plz do not answer question if u don't actually understand thank you

11. You isolated a gene expressed in
differentiated hepatocytes in mice. You then fused
various fragments of the gene (shown as dark
lines in the following figure) to a lacZ reporter
lacking either enhancers or a promoter. The
resulting clones were introduced into hepatocytes
in tissue culture, and the level of lacZ expression
was monitored by using a colorimetric assay.
From the results that follow, which region contains
a likely hepatocyte enhancer?
a. a
a
b b
C
1
d. d
e. e
lacz
expression
100
gooonnons
0
0
5
5
100
5
5
Fragments fused to lacz
lacz
S
d e
✓
abc
promon
Transcribed Image Text:11. You isolated a gene expressed in differentiated hepatocytes in mice. You then fused various fragments of the gene (shown as dark lines in the following figure) to a lacZ reporter lacking either enhancers or a promoter. The resulting clones were introduced into hepatocytes in tissue culture, and the level of lacZ expression was monitored by using a colorimetric assay. From the results that follow, which region contains a likely hepatocyte enhancer? a. a a b b C 1 d. d e. e lacz expression 100 gooonnons 0 0 5 5 100 5 5 Fragments fused to lacz lacz S d e ✓ abc promon
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