12. The bacterium Micrococcus luteus is 3 µm in diameter. What is the diameter in meters? nanometers? Express your answers in both scientific notation and decimal form. Label your answers.
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- 9. Other than bacteria list the other 6 types of Microorganisms listed in your textbook.asap1. What is the most common sterilization technique used in laboratories? 2. List at least 5 procedures of the aseptic technique and describe its uses. 3. Why is Gram stain one of the most important and widely used stains in bacteriology? 4. Explain the Gram staining technique in chronological order. Indicate the reagent used and the time of usage on each reagent.1) would you describe the contents of the soil-inoculated broth as being a “pure culture”? Why or why not? 2) How did the uninoculated broth differ in appearance from the broths inoculated with E. Coli and M. Luteus? And then how could you tell if a supposedly sterile, uninoculated broth was contaminated? Please explain in detail and highlight the important parts cuz I am confused and need help! Thanks
- 1)What is the natural color of cytoplasm? How is it related to our need to stain bacterial cells? 2) Which of the following is the main goal when staining a culture? A) improve contrast B) identify bacteria C) Visualize bacterial structures4) In Gram Stain the bacteria that becomes colorless after washing with alcohol or acetone is the: a) Gram +ve bacteria b) Gram -ve bacteria c) ) Gram neutral bacteria d) archaebacteria e) b & c5) Antimetabolites include: a) Sulfonamides b) Tetracyclins c) Trimethoprim d) all of the above e) a and c6) Oil immersion is used in light microscope because: a) it increases only b) it decreases only c) it increases the / n sin value d) it decreases / n sin e) same refractive index as the glass slide1. Microorganisms are usually measured using in nanometer and microns. Viruses are about only 0.004 to 0.1 μm, while the protozoal cyst can be as large as 50 μm in diameter. What is the protozoal cyst radius in nanometer? (μm= 1 x 10^-6 m, nm = 1 x 10^-9 m) a. 2.5 x 10^-4 nm b. 2.5 x 10^3 nm c. 2.5 x 10^-3 nm d. 2.5 x 10^4 nm 2. Mercy is experiencing a fever. Upon taking her temperature, it was found out that she has a temperature of 101.354 degrees F. How many degrees Fahrenheit is needed to be reduced in order for her temperature to return to normal of 37.1 degrees C? a. 34.54 degrees F b. 34.56 degrees F c. 34.60 degrees F d. 34.57 degrees F 3.7. Room A has a temperature of 103.5 degrees F (39.72 degrees C). Room B has a temperature of 72 degrees F. (22.22 degrees C --> 295.37 K). If room temperature in Room A will be converted into degree Celsius while the temperature in Room B will be in Kelvin scale, what would be the difference if we subtract the converted temperature of room…
- 17. Which of the following statements is correct about the difference between dissecting microscope and compound light microscope? A (3-D) image on compound microscope while (2-D) image on dissecting microscope B. Dissecting microscope requires extremely thin specimen C. Dissecting microscope have larger working distance D. All of the above2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.)1.)What is the purpose of a counterstain? 2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant? 3. True or False? The oil objective should make contact with the oil on the slide. 4. Why is it necessary to let bacterial smears completely air dry before heat fixing? 5.Why should controls be included wherever possible for any staining technique? 6. Why is it necessary to heat the slides while staining for endospores?
- 3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. A. The cell is being viewed under high power. (the pink specimen picture belongs to this question) Scanning power objective=4x; Low power objective= 10x; High power objective= 40x; Eyepiece= 5x; Low power field of view (FOV)= 4.2 mm B. These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. (the two yellow circle specimen picture belongs to this question) Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm1-What can occur if you quickly drop the coverslip onto your wet mount? 2-How can one add stain to the wet mount that has already been prepared? 3-Some live microorganisms can move very quickly. How do we slow these down to view in the microscope?1. Why is it helpful that the transmission compound light microscopes we studied in the lab are both parfocal and parcentered? 2. Why would it be harder to find a specimen on a slide starting with the 40x objective lens compared to the 4x objective lens. Support your answer with facts about the field of view and the comparison of both objective lenses. 3. If I wanted to observe something, let's a virus that was 50nm in size, which microscope would you recommend me using? Support your answer with facts about the microscopic utility.