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- Explain thoroughly the theory of gram differentiation that is based on cell wall structure and lipid component.Figure 9.18 What advantage might biofilm production confer on the S. aureus inside the6)Which of the following statements is a reason why Acid-Fast Bacteria resist the Gram stain a) Because their cell wall architecture varies from both gram-negative and gram-positive bacteria. b) Because they lack the peptidoglycan layer. c) Because they have a thick peptidoglycan layer. d) Because they have a thick layer of fatty acids.
- 1. What is the actual magnification of Cell X? Explain the solution .A. Microscopic Observation of Cells Wet Mounts, Gram stain and spore stain 1 Describe the microscopic morphology and behavior of each species observed with a wet mount (A. serpens and E. gracilis). 2 Describe the microscopic morphology of each sample observed with a Gram stain (S. aureus, P. aeruginosa, B. subtilis and dental plaque). Indicate the Gram reaction of each. 3Describe the microscopic morphology of each sample observed with the spore stain (P. aeruginosa and B. subtilis). In which of the sample can you see spores?Describe the cross - section side view of a Gram - negative cell . Clearly state where the following would be located or write the letters in order of appearance from the outside to the inside . If any of these structures are not present , make sure to leave them out . A ) peptidoglycan ; ( B ) periplasm ; ( C ) porin ; ( D ) LPS ; ( E ) teichoic acids ; ( F ) plasma membrane : ( G ) outer membrane : ( H ) nucleoid
- 1. Please discuss how Gram-Positive and Gram-Negative Cell walls are different from each other. What makes them distinct? How can they be tested and what observations will show from this? 2. You have two microorganism samples and trying to determine characteristics they contain. You determine sample M requires an environment found in a hot spring. While sample P requires rat liver cells to survive. Based upon this information please classify what type of sample M and P are. Provide the term to each and define in detail. (hint for sample P: Q Fever)4. What is the theory about the mechanism of the Gram-stain reaction? 5. What part of the bacterial cell is most involved with Gram staining, and why? Please provide a reference1. why is it necessary to employ a spore stain rather than rely on diagnosis of sporing from examination of gram stain 2.What is the purpose of the bacterial capsule?
- 1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results.1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results. 3. Please provide the scientific name of your microbe that was used in the UV experiment (i.e. S. aureus). Compare your plates and interpret/analyze your results. Please discuss your findings and any patterns you were able to gather. 4. After performing the “Effects of Antiseptics & Disinfectants” lab which agent(s) showed potential to control S. marcescens growth? P. aeruginosa? Please explain why you believe these agent(s) work. 5. What purpose does water serve in the “Effects of Antiseptics & Disinfectants” lab? What did you…1)What is the natural color of cytoplasm? How is it related to our need to stain bacterial cells? 2) Which of the following is the main goal when staining a culture? A) improve contrast B) identify bacteria C) Visualize bacterial structures