2. Scientist discovered how cargo proteins traffic in vesicles by making mutations in genes and observing where the cargo protein gets stuck (using either antibodies that detect the cargo or adding a fluorescen tag on the cargo). Below are a list of mutations in particular genes. Mutation #1 in the adaptin protein Mutation #2 in the Cop I coat protein Mutation #3 in the V-snare found in the Vesicle formed at TGN Given the mutation, predict where you will find the lysosomal protein. Indicate whether A. Protein trafficking is not impacted (you find it in the lysosome) B. Protein will be stuck in the TGN C. Protein will be stuck in the vesicle (somewhere between TGN and lysosome)

2. Scientist discovered how cargo proteins traffic in vesicles by making mutations in genes and observing where the cargo protein gets stuck (using either antibodies that detect the cargo or adding a fluorescen tag on the cargo). Below are a list of mutations in particular genes. Mutation #1 in the adaptin protein Mutation #2 in the Cop I coat protein Mutation #3 in the V-snare found in the Vesicle formed at TGN Given the mutation, predict where you will find the lysosomal protein. Indicate whether A. Protein trafficking is not impacted (you find it in the lysosome) B. Protein will be stuck in the TGN C. Protein will be stuck in the vesicle (somewhere between TGN and lysosome)

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter4: Cells

Section: Chapter Questions

Problem 7TYK: Which of the following statements about proteins is correct? a. Proteins are transported to the...

See similar textbooks

Similar questions

Match the class of yeast mutant with a possible loss of function mutation in a specific protein. v class A 1. cis-Golgi v-SNARE class B 2. COPII v class C 3. the translocator channel or translocon v class E 4. plasma membrane t-SNARE
1d. The csRH mRNA and protein are represented in the diagram below. MRNA 5' AUG 3' ribosome protein signal kinase transmembrane ligand binding domain DNA binding domain sequence domain domain cytoplasm plasma membrane extracellular fluid i. In the boxes on the image, label the N and C termini of the csRH protein. of the csRH protein must be cleaved off before it can be ii. The trafficked along the to the cell membrane. iii. On the schematic above, draw the csRH protein as you would find it at the plasma membrane. Be sure to label the N and C termini of the protein you draw to indicate its orientation. iv. Which protein domains would you NOT expect to find as part of the intracellular hormone H receptor protein icRH?
Match the letter in the diagram with the accumulation or localization of active (or about to be active) protein in a given cellular context, which is wild-type unless specified. CYTOSOL EXTRACELLULAR SPACE A 1. M6P receplor bound to ligand 2. a protein with an (N-SIG] only in a class E yeast mutant 3. plasma membrane v-SNARE 4, clathrin
Which of the situations below will enhance microtubule growth? O addition of a drug that inhibits GDP to GTP exchange on free subunits O addition of a drug that increases the dissociation of GTP-tubulin from the polymer O addition of a drug that inhibits GTP hydrolysis in subunits incorporated into the polymer O None of the above
1)You add a drug to cells that can pass through hydrophilic or hydrobic environments (e.g., membranes & cytosol). The drug specifically prevents the binding of KDEL (ER retention signal) to any other molecules. In the short term, what would happen to the synthesis and localization of translocon proteins? a. New complete translocon proteins would be made, but they would end up in the golgi apparatus. b. New complete translocon proteins would be made, but they would end up at the plasma membrane c. No new intact translocon proteins would be synthesized d. New complete translocon proteins would be made, but they would be secreted e. New complete translocon proteins would be made and localized normally
1. You are interested in the eukaryotic protein/enzyme Thiolase, which is 200 amino acids in length. Because Thiolase is only active in the peroxisome (an organelle) you know there must be a peroxisomal localization signal sequence in the protein. Sub-cellular Location Construct of GFP protein In order to be able to visualize the Thiolase protein in a cell, you create a fusion Cytosol construct with the gene for Thiolase and the GFP gene for Green Fluorescent Protein (GFP). 200 Thiolase Peroxisome GFP You also make a series of deletions in the 50 200 Peroxisome gene for Thiolase, all fused with the GFP GFP 100 200 gene. Numbers on top of the constructs GFP Peroxisome represent amino acid positions. 125 200 Cytosol GFP You express each construct in a mammalian 150 200 cell and use a microscope to visualize where the Thiolase-GFP protein gets trafficked. cytosol GEP 150 Peroxisome GFP Using the data provided, where must the peroxisomal localization signal sequence be in the Thiolase…
Match the letter in the diagram with the accumulation or localization of active or about to be active protein in a given cellular contacts which is wild type unless specified. 1. Plasma membrane v-snare 2. Endosomal v-snare 3. Dynamin 4. A protein with n-sig only in a class e yeast mutant Match with A, B, C, D on diagram
5. How does chloramphenicol inhibits bacterial translation ? Group of answer choices changes shape of 30S portion of the prokaryotic ribosome ; initiates incorrect reading of mRNA codons binds to bacterial t- RNA binds 50 S portion of ribosome; inhibits ribosome translocation bind irreversibly to the small ribosomal subunit binds to large subunit ; inhibits peptidyl transferase activity interferes with attachment of t- RNA to m-RNA in ribosome complex 6. Which of the 2 are NOT target of antibiotics on microbes? Mark both answers Group of answer choices iron uptake lipopolysaccharide synthesis protein synthesis cell wall synthesis plasma membrane injury nucleic acid synthesis
Poliovirus contains a protein that possesses an NLS. A drug is developed that binds and hides this sequence on the viral protein. What happens to this viral protein, in the presence of the drug? Select all that are true. a. The viral protein is now found in the cytoplasm O b. The viral protein is found in the endomembrane system Oc. The viral protein is secreted O d. The viral protein is found in the ER O e. The viral protein can now enter the nucleus
3. Researchers have identified clathrin-coated vesicles that mediate endocytosis and TGN-to-endosome transport, as well as COPII vesicles that mediate ER-to-Golgi transport, as well as COPI vesicles that mediate intra-Golgi transport and Golgi-to-ER transport.But no one has yet identified a general-purpose coat protein involved in creating the secretory vesicles that travel from the TGN to the plasma membrane. Do you think such a coat protein is likely to exist? Explain.
1. The SARS-CoV-2 virus (causing Covid-19) has an RNA genome, which is replicated using an RNA-dependent RNA polymerase (RdRp). The structure below shows a drug that was recently developed as a treatment for infection with SARS-CoV-2 (a) Which molecule normally found in a cell does it most resemble? (You can ignore the part of the structure highlighted with a red box. This is removed by hydrolysis when the drug enters the cell). (b) Suggest a mechanism by which this molecule might inhibit infection. (c) Why is this drug unlikely to have major effects in the host? HN OH OH
1. For each of the following statements regarding protein import into ER Indicate whether it is likely to be true or false. Er likely originated from an aerobic bacterium engulfed by a pre eukaryotic; the signal recognition particle remains associated with the er membrane throughout the import process; most er proteins are fully translated before they are imported into the er; the signal particle is a transmembrane protein in the er Indicate wheater true or false. Uptake of ldl particle requires clathrin; ldl particle are always degraded together with the ldl receptors in the lysosome; in the endosome the ldl particle dissociates from the ldl receptor; a mutation that prevents ldl particle from being digested In the lysosome would cause an increase in cellular cholesterol level; reovvirus enters into the target cell by the fusion of the viral membrane Indicate whether true or false. Lipid synthesis occurs on the inner lipid layer of the er membrane; lipid synthesis occurs in the…