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- Food dye Initial concentration (uM) Initial volume (mL) Final concentration (µM) Final volume (mL) Red 40 32 1.0 C 4.0 Yellow 5 40 3.0 20 Blue 1 8.0 V 1.0 8.0 Cf = µM V mL V = mL * TOOLS II1. What is the purpose of the oil when using oil immersion objective? 2. Why should the oil immersion objective be cleaned immediately after use?3. A torsion prescription balance has a sensitivity requirement of 4 mg. Explain how you would weigh 5 mg of hydromorphone hydrochloride with an error not >5%. Use lactose as the diluent. 4. An electronic balance has a sensitivity requirement of 3.2 mg. Using lactose as the diluent, explain how you would weigh 8 mg of digoxin with an error not >5%. 5. A prescription balance has a sensitivity requirement of 6.5 mg. Explain how you would weigh 20 mg of a substance with an error not >2%.
- 6. Using a balance with a sensitivity of 4 mg, an acceptable weighing error of 5% and cherry syrup as the solvent for tartar emetic, how could you obtain the correct quantity of tartar emetic to fill the prescription?3. Astelin nasal spray contains 0.1% azelastine hydrochloride and 400 µg/mL of benzalkonium chloride as a preservative. A container is capable of delivering 200 metered sprays of 0.137 mL each. The percent concentration of benzalkonium chloride in the preparation is ________ %, and this is equivalent to a ratio strength of 1:2500 w/v. True or False?4. On a TLC plate containing silica gel, which compound will have the largest Rf value when ethyl acetate is used as the mobile phase? Circle your answer for credit. Compound A CH3 cor CH3 H₂C COOH Compound B H.C. 0 CH,
- 1. What is the purpose of adding immersion oil when using the 100X objective?AY AY * 00 HI T 00 2 3. ment10: Connect Regis.. M https://Ims.mh.. nemical Content of Media i Saved Help Save & Exit Chec Chemically defined medium Complex medium 2 Enriched medium Fastidious Heterotroph 9 Autotroph Match each of the options above to the items below. Uses inorganic carbon as a carbon source for building its own organic molecules. A type of organism that has complex nutrient requirements and requires a nutritionally dense environment. A media containing extracts from other other organisms. A medium with specifically added nutrients to enhance the growth of fastidious organisms. An organism that uses organic carbon as its carbon source. A medium of which the exact chemical composition is known. MacBook Air DD F7 000 000 F2 F4 F5 F8 %23 2$ 4. 9- } 1 Y | 7.7. The spectrophotometer is used to measure absorbance experimentally. Briefly, explain how this is accomplished.
- 2. Imagine you have mixed the following known concentrations of a solution. You then measured their absorbance in the spectrophotometer. Construct a standard curve (a line) from the following data points. Use the graph below. Each axis must be labeled with the variable and units, and the scale must be evenly spread. Tube Known concentration (M) Absorbance (AU) 1 0. 0.1 0.03 0.5 0.06 1.0 0.08 5 5.0 0.28 Eseel 222. Calculate the following: a. you are asked to prepare 10 NA slants and 5 NA stab in big tubes. How much NA should you prepare if the indicated preparation for NA is as follows: 1. Suspend 28g of nutrient agar powder (CM0003B) in 1L of distilled water. 2. Mix and dissolve them completely. 3. Sterilize by autoclaving at 121°C for 15 minutes.The primary concern with using bleached paper products for food is: O lead O dioxin O methylmercury O pesticides O microorganisms