20-22. TBX5 is a gene found in vertebrates which is expressed in the heart and forelimbs during development. Parts of the first and last coding exons of the human TBX5 gene are shown below, with start and stop codons in bold: Partial first coding exon: 5' -ACCATGGCCGACGCAGACGAGG-3' Partial last exon: 5'-GTGGAGCGACAATAGCTAAAGT-3' 20. Which primer pair would you use to amplify full length TBX5 coding sequence? a. 5'-ATGGCCGACGCAGACGAGG-3'and 5'-GTGGAGCGACAATAGCTAA-3' b. 5'-ATGGCCGACGCAGACGAGG-3'and 5'-TTAGCTATTGTCGCTCCAC-3' c. 5'-ATGGCCGACGCAGACGAGG-3'and 5'-AATCGATAACAGCGAGGTG-3' d. 5'-CCTCGTCTGCGTCGGCCAT-3'and 5'-GTGGAGCGACAATAGCTAA-3' e. 5'-GGAGCAGACGCAGCCGGTA-3'and 5'-GTGGAGCGACAATAGCTAA-3'

20-22. TBX5 is a gene found in vertebrates which is expressed in the heart and forelimbs during development. Parts of the first and last coding exons of the human TBX5 gene are shown below, with start and stop codons in bold: Partial first coding exon: 5' -ACCATGGCCGACGCAGACGAGG-3' Partial last exon: 5'-GTGGAGCGACAATAGCTAAAGT-3' 20. Which primer pair would you use to amplify full length TBX5 coding sequence? a. 5'-ATGGCCGACGCAGACGAGG-3'and 5'-GTGGAGCGACAATAGCTAA-3' b. 5'-ATGGCCGACGCAGACGAGG-3'and 5'-TTAGCTATTGTCGCTCCAC-3' c. 5'-ATGGCCGACGCAGACGAGG-3'and 5'-AATCGATAACAGCGAGGTG-3' d. 5'-CCTCGTCTGCGTCGGCCAT-3'and 5'-GTGGAGCGACAATAGCTAA-3' e. 5'-GGAGCAGACGCAGCCGGTA-3'and 5'-GTGGAGCGACAATAGCTAA-3'

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter15: Genes And Proteins

Section: Chapter Questions

Problem 26CTQ: Chronic lymphocytic leukemia patients often harbor nonsense mutations in their spliceosome...

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