9. The following sequence is a wild-type bacterial gene that encodes a short protein. The sequence given is from the point where transcription starts (called "+1") to the point where transcription ends. Transcription goes left to right. 5'-ACTTCGATATGTCTAAAATATCGATCGATCTGTGGGGCCTAGCTAGCTAACCAGAGACGCTACCG-3' 3'-TGAAGCTATACAGATTTTATAGCTAGCTAGACACCCCGGATCGATCGATTGGTCTCTGCGATGGC-5' (a) Which strand (the upper or the lower) is used as the template in transcription? (b) Write out the first 20 bases of the RNA transcribed from this wild-type gene. Make sure to label the 5' and 3' ends of your molecule. (c) Write out the amino acid sequence of any protein that is encoded by this wild-type gene. Make sure to label the N and C termini of your molecule.
Q: 8. The following diagram represents the Christmas-tree-like structures during transcript On the…
A: E. Eliminators are viewed downstream of the quality as deciphered, and ordinarily happen…
Q: 4. Which mRNA sequence complements the DNA sequence below? (LS1-1)
A: DNA is a polynucleotide strand. DNA is transcribed into RNA and RNA is translated into proteins.…
Q: 2. Shown below is the DNA sequence of a eukaryotic gene that encodes a short peptide. The sequence…
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A: According to bartleby expert guidelines, when multiple questions are posted we are allowed to answer…
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A: The genetic information of all living organisms (except some viruses) is stored in the cell in the…
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A: Ribosomal and transfer RNAs are processed in both prokaryotes and eukaryotes. The processing of…
Q: 1. The nontemplate strand of a segment of double- helical DNA contains the sequence:…
A: Since you have posted a question with multiple sub-parts, we will solve the first 2subparts for you.…
Q: 1. Determine what is being meant by each statements: a. It describes mRNA that results in a single…
A:
Q: 5.Below is schematic of gene Z, which encodes for protein Z. the promoter is indicated by the dotted…
A: Given information In the diagram , there are Exon 1, Exon 2 and Exon 3. There are two introns 1 and…
Q: 1. Which is the correct of mRNA strand if you have a tRNA of GCA-AUG-UCC-CGU? A.…
A: Introduction Genetic code or codon is a three letters nucleotide bases present on m RNA which code…
Q: DNA Leading strand: 5' AAA ATA | CGC TTT| TTA ATT | AAC CCC GGG 3' A I B |C| D Exons: A, C, D…
A: The process of synthesizing RNA from the genetic information encoded by DNA is called Transcription…
Q: 6. How many amino acids will the mRNA sequence "AUG GAC CUG UCG UGA" produce? (LS1-1) * Second MRNA…
A: The translation is the process by which the information on the mRNA is transferred into appropriate…
Q: Why aren't ssb proteins necessary in transcription?
A: The function of SSB protein is to prevent the recoiling of parent strands during replication. As we…
Q: b) Shown below is a very short gene of an unknown bacteria genome (Figure 2). Transcription starts…
A: The mRNA is produced from the templates stand of DNA by the transcription process and polypeptide…
Q: 1. Classify the type of mutation that have taken place: silent, missense and nonsense as a result of…
A: Introduction: The changes or alteration in the sequence of DNA is known as mutation.
Q: 5. What is the correct reading frame for the following mRNA? MRNA 5' GGCACUUAUGCGAUGCCUUGAGUGACCAU…
A: mRNA is made from DNA by the process of Transcription.
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A: Here I will provide you first 10 nucleotides long mRNA sequences according to question.
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Q: Hello, my question is in the picture below. Thank you in advance!
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Q: Shown here is a DNA sequence. The promoter is highlighted in yellow and the terminator is…
A: Given: Sequence of template DNA is…
Q: Which of the following is a true statement concerning condons
A: Codons are nucleotide triplets that are read together in order to specify amino acids during…
Q: What are the components of the initiation complex for translation
A: Translation is a process in which codon of mRNA code for specific amino acid. These amino acid…
Q: Can you please answer number 24
A: Answer 24. Genetic code is a set of rules used by living cells to translate information encoded…
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A: Introduction Transcription : It Is The Initial Stage In Gene Expression, Which Involves "The…
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A: Gene expression allows the cell to respond to various environments. Transcripted DNA (mRNA) may have…
Q: 1. The nontemplate strand of a segment of double- helical DNA contains the sequence:…
A: DNA => Transcription => mRNA => Translation => Protein. Given : Non-template strand…
Q: Shown is a segment of DNA with its promoter and terminator. Start and end of transcription are…
A: Transcription is the phenomenon in which one stranded RNA is synthesized from DNA strand . But RNA…
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A: The correct option is (C) a sequence upstream of the AUG initiation codon on mRNA.
Q: 8. The CAS9 enzyme (shown in red in the image below arget and cut out specific DNA sequences.
A: As you have not mentioned which part to answer, we are answering first three for you. If you want…
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A: Ans ) Always Guanine codes for cysteine only .. That is G-C Given.…
Q: 2. How is RNA termination different in prokaryotes vs eukaryotes? Include an explanation of cis vs.…
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Q: 2. A reversion is a mutation that returns a mutant codon back to a codon that gives a wild-type…
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Q: shown below is a schematic drawing of a gene, with the transcription unit divided into numbered…
A: A gene is a stretch of nucleotides present in the DNA. DNA or deoxyribonucleic acid is a polymer of…
Q: 1. What happens during transcription? Possible sentence frame: Transcription is the process in which…
A: Need to fill the blanks related to transcription process.
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A: In the question, we must write the complementary base of the sequence. Our mRNA sequence will be in…
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A: ANSWER;- a) The sequence of amino acids in the following structure Met-Phe-Leu-Ser-Thr-Pro b)(i) DNA…
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- Diamond–Blackfan anemia (DBA) is a rare, dominant geneticdisorder characterized by bone marrow malfunction, birthdefects, and a predisposition to certain cancers. Infants withDBA usually develop anemia in the first year of life, have lower thannormal production of red blood cells in their bone marrow, and havea high risk of developing leukemia and bone cancer. At the molecularlevel, DBA is caused by mutations in any one of 10 genes thatencode ribosomal proteins. The first-line therapy for DBA is steroidtreatment, but more than half of affected children develop resistanceto the drugs and in these cases, treatment is halted. DBA canbe treated successfully with bone marrow or stem cell transplantsfrom donors with closely matching immune system markers. Transplantsfrom unrelated donors have significant levels of complicationsand mortality. A couple with a child affected with DBA undergoes in vitro fertilization (IVF) and genetic testing of the resulting embryos to ensure that the embryos…Diamond–Blackfan anemia (DBA) is a rare, dominantgenetic disorder characterized by bone marrow malfunction,birth defects, and a predisposition to certaincancers. Infants with DBA usually develop anemia in the firstyear of life, have lower than normal production of red blood cellsin their bone marrow, and have a high risk of developing leukemiaand bone cancer. At the molecular level, DBA is causedby mutations in any one of 10 genes that encode ribosomalproteins. The first-line therapy for DBA is steroid treatment,but more than half of affected children develop resistance tothe drugs and in these cases, treatment is halted. DBA can betreated successfully with bone marrow or stem cell transplantsfrom donors with closely matching immune system markers.Transplants from unrelated donors have significant levels ofcomplications and mortality. While a stem cell transplant from an unaffected donor is currentlythe only cure for DBA, genome-editing technologies mayone day enable the correction of…Diamond–Blackfan anemia (DBA) is a rare, dominantgenetic disorder characterized by bone marrow malfunction,birth defects, and a predisposition to certaincancers. Infants with DBA usually develop anemia in the firstyear of life, have lower than normal production of red blood cellsin their bone marrow, and have a high risk of developing leukemiaand bone cancer. At the molecular level, DBA is causedby mutations in any one of 10 genes that encode ribosomalproteins. The first-line therapy for DBA is steroid treatment,but more than half of affected children develop resistance tothe drugs and in these cases, treatment is halted. DBA can betreated successfully with bone marrow or stem cell transplantsfrom donors with closely matching immune system markers.Transplants from unrelated donors have significant levels ofcomplications and mortality. Given that a faulty ribosomal protein is the culprit and causesDBA, discuss the possible role of normal ribosomal proteins.Why might bone marrow cells be…
- Which of the following set(s) of primers a–d couldyou use to amplify the following target DNA sequence, which is part of the last protein-coding exonof the CFTR gene?5′ GGCTAAGATCTGAATTTTCCGAG ... TTGGGCAATAATGTAGCGCCTT 3′3′ CCGATTCTAGACTTAAAAGGCTC ... AACCCGTTATTACATCGCGGAA 5′a. 5′ GGAAAATTCAGATCTTAG 3′;5′ TGGGCAATAATGTAGCGC 3′b. 5′ GCTAAGATCTGAATTTTC 3′;3′ ACCCGTTATTACATCGCG 5′c. 3′ GATTCTAGACTTAAAGGC 5′;3′ ACCCGTTATTACATCGCG 5′d. 5′ GCTAAGATCTGAATTTTC 3′;5′ TGGGCAATAATGTAGCGC 3′2. Null mutations are valuable genetic resources becausethey allow a researcher to determine what happens to anorganism in the complete absence of a particular protein. However, it is often not a trivial matter to determinewhether a mutation represents the null state of the gene.a. Geneticists sometimes use the following test forthe nullness of an allele in a diploid organism: If theabnormal phenotype seen in a homozygote for theallele is identical to that seen in a heterozygote(where one chromosome carries the allele in question and the homologous chromosome is known tobe completely deleted for the gene) then the alleleis null. What is the underlying rationale for thistest? What limitations might there be in interpreting such a result?Price et al. [(1999). J. Bacteriol. 181:2358–2362] conducteda genetic study of the toxin transport protein (PA) of Bacillusanthracis, the bacterium that causes anthrax in humans. Withinthe 2294-nucleotide gene in 26 strains they identified five pointmutations—two missense and three synonyms—among differentisolates. Necropsy samples from an anthrax outbreak in 1979revealed a novel missense mutation and five unique nucleotidechanges among ten victims. The authors concluded that thesedata indicate little or no horizontal transfer between differentB. anthracis strains. Question: Which types of nucleotide changes (missense or synonyms)cause amino acid changes?
- Price et al. [(1999). J. Bacteriol. 181:2358–2362] conducteda genetic study of the toxin transport protein (PA) of Bacillusanthracis, the bacterium that causes anthrax in humans. Withinthe 2294-nucleotide gene in 26 strains they identified five pointmutations—two missense and three synonyms—among differentisolates. Necropsy samples from an anthrax outbreak in 1979revealed a novel missense mutation and five unique nucleotidechanges among ten victims. The authors concluded that thesedata indicate little or no horizontal transfer between differentB. anthracis strains. Question: On what basis did the authors conclude that evidence ofhorizontal transfer is absent from their data?Price et al. [(1999). J. Bacteriol. 181:2358–2362] conducteda genetic study of the toxin transport protein (PA) of Bacillusanthracis, the bacterium that causes anthrax in humans. Withinthe 2294-nucleotide gene in 26 strains they identified five pointmutations—two missense and three synonyms—among differentisolates. Necropsy samples from an anthrax outbreak in 1979revealed a novel missense mutation and five unique nucleotidechanges among ten victims. The authors concluded that thesedata indicate little or no horizontal transfer between differentB. anthracis strains. Question: What is meant by ”horizontal transfer”?What Art the Features of the Series of -omes? Define the following terms: a. Genome b. Transcriptome c. Proteome d. Metabolome e. Fluxome
- Utilizing Hind III and EcoR V Restriction Enzyme with Pet41 and the following gene of interest... a tgaaacaaca aaaacggctt tacgcccgat tgctgacgct gttatttgcg 61 ctcatcttct tgctgcctca ttctgcagca gcggcggcaa atcttaatgg gacgctgatg 121 cagtattttg aatggtacat gcccaatgac ggccaacatt ggaagcgttt gcaaaacgac 181 tcggcatatt tggctgaaca cggtattact gccgtctgga ttcccccggc atataaggga 241 acgagccaag cggatgtggg ctacggtgct tacgaccttt atgatttagg ggagtttcat 301 caaaaaggga cggttcggac aaagtacggc acaaaaggag agctgcaatc tgcgatcaaa 361 agtcttcatt cccgcgacat taacgtttac ggggatgtgg tcatcaacca caaaggcggc 421 gctgatgcga ccgaagatgt aaccgcggtt gaagtcgatc ccgctgaccg caaccgcgta 481 atttcaggag aacacctaat taaagcctgg acacattttc attttccggg gcgcggcagc 541 acatacagcg attttaaatg gcattggtac cattttgacg gaaccgattg ggacgagtcc 601 cgaaagctga accgcatcta taagtttcaa ggaaaggctt gggattggga agtttccaat 661 gaaaacggca actatgatta tttgatgtat gccgacatcg attatgacca tcctgatgtc 721 gcagcagaaa ttaagagatg gggcacttgg tatgccaatg aactgcaatt ggacggtttc 781…The human RefSeq of the entire first exon of a geneinvolved in Brugada syndrome (a cardiac disordercharacterized by an abnormal electrocardiogram andan increased risk of sudden heart failure) is:5′ CAACGCTTAGGATGTGCGGAGCCT 3′The genomic DNA of four people (1–4), three ofwhom have the disorder, was subjected to singlemolecule sequencing. The following sequences represent all those obtained from each person. Nucleotidesdifferent from the RefSeq are underlined. Individual 1:5′ CAACGCTTAGGATGTGCGGAGCCT 3′and5′ CAACGCTTAGGATGTGCGGAGACT 3′Individual 2:5′ CAACGCTTAGGATGTGAGGAGCCT 3′Individual 3:5′ CAACGCTTAGGATGTGCGGAGCCT 3′and5′ CAACGCTTAGGATGGCGGAGCCT 3′Individual 4:5′ CAACGCTTAGGATGTGCGGAGCCT 3′and5′ CAACGCTTAGGATGTGTGGAGCCT 3′a. The first exon of the RefSeq copy of this gene includes the start codon. Write as much of the aminoacid sequence of the encoded protein as possible,indicating the N-to-C polarity.b. Are any of these individuals homozygotes? If so,which person and what allele?c. Is…Robert Bost and Richard Cribbs studied a strain of E. coli (araB14)that possessed a nonsense mutation in the structural gene that encodes Lribulokinase,an enzyme that allows the bacteria to metabolize the sugararabinose (R. Bost and R. Cribbs. 1969. Genetics 62:1–8). From thearaB14 strain, they isolated some bacteria that possessed mutations thatcaused them to revert back to the wild type. Genetic analysis of theserevertants showed that they possessed two different suppressormutations. One suppressor mutation (R1) was linked to the originalmutation in L-ribulokinase and probably occurred at the same locus. Byitself, this mutation allowed the production of L-ribulokinase, but theenzyme produced was not as effective in metabolizing arabinose as theenzyme encoded by the wild-type allele. The second suppressormutation (SuB) was not linked to the original mutation. In conjunctionwith the R1 mutation, SuB allowed the production of L-ribulokinase, butSuB by itself was not able to suppress the…