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- 4. Why is 70% ethanol rather than pure ethanol preferred for use as an antiseptic agent? BARTLEBY3. Calculate the total volume of the diluent that you are going to use if you have prepared a 3% red cell suspension using 0.5 mL of pRBC.1. Prepare 200 mL of an isotonic aqueous solution of thimerosal (MW 404.84; L iso 3.4). The concentration of this antiinfective drug is 1:5000. a. Calculate the E value. b. Adjust the Isotonicity of the solution using NaCl.
- Agarose gels can vary from 0.8 to 2% explain why we use different gel percentages1. When can gelatin be utilized in culture media preparation? 2. Why should Erlenmeyer flasks hold not more than 60% of their capacity? 3. Why is a lower concentration of NaOH or HCl used for titration while a solution with higher concentration is used for adjusting the pH of the remaining medium?1. Which of the following statement/s is/are TRUE for the protein sample?* The sample will give a positive result to Biuret test. All of A, B and C The sample will give a positive result to Ninhydrin test. The sample will give a positive result to Xanthoproteic test. 2. Extremely high pH causes folding of the protein molecules. * The statement is CORRECT. The statement is INCORRECT. 3.Negative with Biuret Test but positive with Ninhydrin Test, Xanthoproteic Test and Millon’s Test Glycine Tryptophan Tyrosine Methionine Albumin
- 1. Why does fresh milk can form a film and sour milk cannot?1.Which of the sample dyes are negatively charged at neutral pH? 2.Which of the sample dyes are positively charged at neutral pH? 3.1: Did Orange G, Xylene Cyanol and Safranin O move the same distance in the gel? 3.2 : Why or why not? What compounds do you suspect are in the unknown sample? Explain.I. Identify the approximate amount of the visible band II. Describe the trouble shooting problem from the gel
- 1. What volume of sterile water for injection (diluent) must be added to reconstitue the powder? 2. How many mL of the reconstituted cefazolin is needed to prepare the final product?You have a sample at 50 ng/ul and you would like to load 400ng of this sample on a lane of an agarose gel. You also have TE buffer as diluent and 6x loading dye. Your total sample should be 12ul. Calculate the amounts of each reagent necessary to prepare this sample for gel loading.When acids are added to a solution, the pH should ___________ . a. decrease b. increase c. stay the same d. cannot tell without testing