A biochemist carefully measures the molarity of salt in 56. mL of photobacterium cell growth medium to be 82. mM. Unfortunately, a careless graduate student forgets to cover the container of growth medium and a substantial amount of the solvent evaporates. The volume of the cell growth medium falls to 3.1 mL. Calculate the new molarity of salt in the photobacterium cell growth medium. Be sure your answer has the correct number of significant digits. mM
Q: The column efficiency is constant for a column that uses a cons tant eluent composition. What does…
A: The question is related to chromatography which helps to separates compounds from a mixture of…
Q: 2 L of 5 mg/mL gelatin solution from 1 g/mL gelatin solution
A: Gelatin is a food ingredient that is derived from collagen. It is basically a protein in nature.
Q: Complete the graph in generation time and answer questions in no.1
A: The generation time of bacteria refers to the time of bacteria to double in a bacterial culture. A…
Q: What are the mechanisms of samples separation work in Thin layer Chromatography?
A: Chromatography is a physicochemical technique for the division of compound mixtures, in view of the…
Q: Kael needs to prepare Luria Bertani Agar for 40 plates, 15 slants (big tubes), and 15 stabs (small…
A: LBA agar is used for cultivation of recombinant E.coli. it is a type of rich medium it is also used…
Q: When 0.2 millimoles of Y are dissolved in 250 ml of water, the resultant solution has an absorbance…
A: Beer-lambert law states that for the given material, the sample concentration and path length are…
Q: During extractions, it is common to add a dessicant/drying agent (such as magnesium sulfate) to…
A: Drying agents, also called as desiccants, play a major role in removal of water and other polar…
Q: You are tasked to prepare 1000mL of 2 media preparations, however, instead of using a powder agar,…
A: Media is providing the nutrients for growth of micro organism.
Q: ing is a recipe for a bacterial growth medium: 35 g Dextrose, 12 g Aga 1.2g Salt, 3 g Yeast cell…
A: Growth medium can be of many types. Growth media is a media which contains nutrients for chemical…
Q: You must make a choice between two chromatographic systems for the analysis of a specific compound.…
A: Partition coefficient can be determined as the ratio of the two-phase that is the stationary and…
Q: What would be the best buffering agent to choose if you wanted to buffer an enzyme reaction or…
A: pH is the measure of the strength of H+ ion or Hydronium ions in solution. pOH is the measure…
Q: You have 200 mL of a 1.5 M glucose solution. How many moles of glucose are there?
A: Introduction: Glucose is a monosaccharide and a major source of energy for the living body. The…
Q: Given the following common laboratory materials, which of the three methods of heat sterilization do…
A: Three Methods of heat sterilisation include: Burning over flame Wet heat (autoclave) Dry heat…
Q: Why is it important to select immiscible solvents when performing liquid-liquid extraction? In…
A: Liquid-liquid extraction is a process of separating components present in solutions by their…
Q: What nutrients do the following media components contain? Peptone Yeast extract Beef…
A: “Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…
Q: Calculate the volume of BSA stock that will be required to make the standard solutions needed to…
A: A stock solution is a concentrated solution that is diluted to make a working solution. It is used…
Q: You want to feed a new flask @: 2 x 105 cells/mL, 8 mL total per flask. Describe how you dilute your…
A: Viable or the live cells can be counted by the instrument called the hemocytometer. These live cells…
Q: Why should Erlenmeyer flasks hold not more than 60% of their capacity? When can gelatin be used in…
A: 1*Erlenmeyer flasks are used to contain liquids and for mixing and heating and cooling, incubation…
Q: If you added 5 mL of a 20X tocopherol to 5 mL of cell culture at a density of 2.4 x 107 cells/mL,…
A: For calculating the concentration of a solution, the formula used is M1V1=M2V2 Where, M1 =…
Q: You have a 20 mg/mL stock solution of the amino acid arginine and need to make plates containing…
A: In biochemistry, a stock solution is a high volume of a standard reagent. They're utilized for a…
Q: Consider a mixture of two proteins with molecular weights of 20,000 and 200,000. For simplicity of…
A: Sedimentation Coefficient is the rate per unit centrifugal field experienced by the particle…
Q: What wavelength is most suitable for quantitative analyses for an analyte exhibiting the UV-Vis…
A: Quantitative analysis is the method of determination of concentration of an analyte where teh amount…
Q: Given the following common laboratory materials, which of the three methods of heat sterilization do…
A: Sterilization is the used to kill the microorganisms or other pathogens from a substance or…
Q: If you had a 50 mL volume of 1% agarose gel to create with appropriate amount of SYBR Gold how would…
A: In molecular biology, agarose gel is used in electrophoresis to separate the DNA and RNA molecules…
Q: What will be the results after you reconstitued a cefixime 60 ml a powder for suspension and stand…
A: A coarse dispersion suspension is a system in which an internal phase(also called the solid…
Q: If I add 10 microliters of a culture at 106 cells/mL to 990 microliters of nutrientbroth, what is…
A: Microbiology is the branch of science that studies microscopic organisms. As microorganisms are not…
Q: Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that…
A: The countable range of colonies for an ideal plate count method is considered 30-300 colonies per…
Q: You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis.…
A: TAE buffer used in agarose gel electrophoresis for the seperation of DNA and RNA.
Q: What is the chemistry principle behind when (treatment: heavy cream) is subjected to room…
A: Heavy cream temperature is important for the physical characteristics of the cream. At room…
Q: For microprojectile bombardment, the particles used are typically? a. Nickel b. Tungsten O c.…
A: Ttransformation is a biotechnological process where a foreign DNA molecule is inserted into the host…
Q: SEE EXAMPLE IN THE IMAGE A mixture of 0.20M acetic acid and 0.30M sodium acetate is given. Calculate…
A: The Henderson Hasselbalch equation for calculation of the pH of a solution of weak acid and its…
Q: A sample to be used for medical imaging is labeled with 18F,which has a half-life of 110 min. What…
A:
Q: You have 12 mL of cells you need to treat with Hydrogen Peroxide (H2O2), such that the final…
A: Given , Volume of cells = 12 mL Final concentration of H2O2 = 50 uM The amount of 30 mM stock…
Q: When a mixture of various molecular sized dyes are run through a gel chromatography column, what is…
A: Chromatography is a process of separation of substances from mixtures based on their molecular size.…
Q: Measurement of Bacterial Growth in LB, pH 3.2 Plot of Absorbance vs. Time (mins) c 37c 45'c 0.6 0.4…
A: The bacterial growth curve consists of four distinct growth phases: Lag Phase, Exponential Phase,…
Q: Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium…
A: The LB agar medium is designed in such a way that it is nutrient rich which supports the growth of…
Q: If I add 10 microliters of a culture at 106 cells/mL to 990 microliters of nutrient broth, what is…
A: Cell density refers to number of cells present per unit volume. It is usually expressed in cells/mL.
Q: 5 L of 0.2 M dextrose solution from 5 M dextrose solution
A: Given Values: Stock solution of dextrose = 5 M Final solution of dextrose = 0.2 M Volume of the…
Q: Calculate the amount of each solution needed to make 750 mL if a dextrose 25% solution given only a…
A: Given- We make 25% dextrose of 750ml by using 60% and 5% dextrose. Use alligation method for such…
Q: From a 50% solution of dextrose, make 500mL of a 5% solution. Your answer
A: We are told that we have a 50% solution of dextrose . A 50% solution means that there are 50 g of…
Q: Why 70% Alcohol is better than 100% Alcohol (as an antiseptic) to control the growth of bacteria?
A: Sterilization is process of destroying all the viable microbes including viruses, endospores etc.…
Q: Solution A is 20 degrees celsius, Solution B is 80 degrees celsius (both are the same kind of…
A: The amount of heat required per unit mass to increase the temperature by one degree Celsius is known…
Q: Two compounds have Rf values of 0.50 and 0.61, respectively. The solvent front on a plate is…
A: Chromatography technique is used to separate mixtures. In this technique, there are 2 phases,…
Q: The following are denaturing agents for proteins, except:* Please choose one correct answer only.…
A: Proteins are composed of twenty standard amino acids that are attached together via peptide bonds.…
Q: A biochemist carefully measures the molarity of salt in 56. mL of photobacterium cell growth medium…
A: GIVEN: The initial volume of the photobacterium cell growth medium (V1)= 56 mL Concentration of the…
Q: 1. When can gelatin be utilized in culture media preparation? 2. Why should Erlenmeyer flasks hold…
A: Gelatin is employed in culture media to determine bacteia gelatinolysis (gelatinase elaboration).…
Q: You are a graduate student who is trying to conduct an experiment. For this experiment, you need to…
A: In order to make working solutions, it is often required to dilute the available stock solutions.…
Q: Briefly describe how you would make 500 mls of a 1X electrophoresis running buffer from a 15X stock…
A: Electrophoresis buffer: Electrophoresis buffer can be Tris-acetate EDTA (TAE) or Tris-borate EDTA…
Q: If the agar instructions say to mix 50 grams of agar in 1 liter of water, how many grams of agar…
A: 1 litre/ 1000 mL needs 50 grams of agar 1 mL needs 50/1000= 0.05 grams of agar 100 mL needs 0.05 X…
Q: A 10,000L bioreactor may have 100 trillion cells. Let’s assume that this cell solution has the same…
A: Hi, as you have posted multiple sub-parts and have not mentioned which one is to be answered, we are…
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- If you added 5 mL of a 20X tocopherol to 5 mL of cell culture at a density of 2.4 x 107 cells/mL, what would the final concentration of tocepherol and cell density be?If a broth culture of bacteria, initially containing 37,000 bacteria/ml, were diluted to a 1:1,000 dilution, how many bacteria/ml of the diluted broth would be present on average? Show your solutionsA 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB medium to obtain a final concentration of 50µg/ml ofampicillin?
- Show the calculations required to make up 250mlof a stock solution of this chemical that will then be at the working concentration when 1 volume of this solution is added to 10 volumes of cell culture medium.If I add 10 microliters of a culture at 106 cells/mL to 990 microliters of nutrient broth, what is the cell density in the new solution?If I add 10 microliters of a culture at 106 cells/mL to 990 microliters of nutrientbroth, what is the cell density in the new solution?
- Answer the ff with not more than two sentences: Why should Erlenmeyer flasks hold not more than 60% of their capacity? When can gelatin be used in culture media preparation? How does cotton plug prevent contamination of the medium? Why should caps of glassware be loosened first before sterilization?A culture medium is comprised of the following components: 0.5% peptone, 0.3% meat extract, 1.5% agar, and distilled water. If you are asked to prepare 765 mL of culture media, how much meat extract would you need?Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into account the dilution factor as per question 6, and the total volume of extract as per question 4. Note your answer to 2 decimal places Sample 1 Total Extract Volume (ml) is 45 Dilution factor is 100 A280 is 1.07 A620 is 0.221 A650 is 00.97 Specific Absorbance Ratio (SAR) A620/A280 is 0.206
- in food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase activity on hydrogen peroxide at different pH values? (wavelength used = 415nm). can you please explain the calculations thank you if given: Temperature = 75 C Molar absorptivity coefficient = 10.5 Path length (cm) = 2 I = transmitted light = 0.45You are growing yeast cells in your lab. The Yeast Growth Medium contains 56 mM glucose (Mol. Weight 180 g/mol) and 20 mM HEPES (MW 238 g/mol). The yeasts only grow if the medium is at the exact pH=6.8. You have HEPES and glucose powder, water, hydrochloric acid (HCl) and Sodium hydroxide (NaOH), all the necessary equipment to prepare the 100 ml that you need. Calculate and explain how you make the Yeast Growth Medium:What nutrients do the following media components contain? Peptone Yeast extract Beef extract Potato infusion Agar Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? 1.Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?Please include source