3.0 2.5 2.0 1.5 1.0 0.5 ● ● b a b a bab a b 1 2 3 A. Which is the more polar compound? B. Which is the most polar solvent system? C. Which solvent system (s) would be the best choice to elute compound "b" from a flash column? solvent front 5 origin solvent system
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- I don't understand how you calculated the w1/2 for part c... where did the 5.54 come from? And can you answer the last part of the question-> d) If the volume ratio of the stationary phase to mobile phase is 0.25, find the distribution constant for compound 1.Indicate the order of elution in which the following compounds would be eluted from an HPLC column containing a reversed-phase packing: (a) benzene, diethyl ether, n-hexane (b) propylene, hexane, benzene, dichloro-benzenewhat are key reactions involved in seperation of liquid mixtures by simple and fractional distillation and gas chromatography? chemicals used: 10ml 1:1 mixture of ethyl and butyl acetate techniques: simple and fractional distillation, gas chromatography. target molecules: Ethyl acetate C4H8O2 Butyl acetate C6H12O2
- Calculate the vapor pressure of propanol (b.p. 97.0 ∘C97.0 ∘C) at the gas chromatography column temperature of 95.0 ∘C95.0 ∘C using the form of the Clausius–Clapeyron equation shown ln(?1?2)=−(Δ?vap?)(1?1−1?2)ln(P1P2)=−(ΔHvapR)(1T1−1T2) where ?R is the ideal gas constant, Δ?vapΔHvap is the enthalpy of vaporization, ?1T1 and ?2T2 are two different temperatures, and ?1P1 and ?2P2 are the vapor pressures at the respective temperatures. The enthalpy of vaporization can be estimated using Trouton's rule, Δ?∘vap=(88 J·mol−1⋅K−1)×?bp.ΔHvap∘=(88 J·mol−1⋅K−1)×Tbp. ?=P= TorrTorr Calculate the vapor pressure of pentane (b.p. 36.0 ∘C36.0 ∘C) at the same column temperature. ?=P= TorrTorr For compounds of the same chemical class, what is the relationship between vapor pressure of a compound and retention in gas chromatography? As the vapor pressure decreases, retention time decreases. As the vapor pressure increases, retention time increases. As…A TLC plate showed two spots A and B with Rf 0.20 and 0.30, respectively. Sketch a TLC chromatogram with the above Rf values. (Hint: Start with a 10-cm TLC plate and allow 1 cm each for the start and end solvent fronts.) Determine the distances travelled from the start of the solvent front. ___?____ A ____?___ B Determine the distances left to the end of the solvent front. ___?____ A ____?___ B4g 5 g 2 g 100 g 49." R Lactic acid Salicylic acid Trichloroacetic acid Flexible collodion qs ad Sig: wart remover. Use as directed. (a) Flexible collodion contains 20% w/w camphor and 30% w/w castor oil. How many grams of each would be contained in 30 g of the mixture? (b) The specific gravity of castor oil is 0.955. How many milliliters of the oil is contained in 30 g of the mixture? (c) If the specific gravity of the mixture is 0.781, what are the percent w/v con- centrations of lactic acid, salicylic acid, and trichloroacetic acid in the mixture?
- Use the following information collected from a SIZE-EXCLUSION CHROMATOGRAPHY experiment to answer the questions below. MW Standards Molecular Weight (kDa) Elution Volume (mL) blue dextran 1000 85 myoglobin 17.2 200 TIM 53.3 178 hemoglobin 62.2 156 IgG 140 122 ATCase 307.9 94 Prepare a graph showing the linear relationship between log function of molecular weight against Ve/Vo. The R2value must be 0.9 or higher. What is the linear equation from the standards given? What is the R2value?Alfred was tasked to synthesize at least 250.0 ppm of his novel gold nanoparticles (AuNP’s) to be able to perform experiments about the efficacy of AuNP for viral detection. He decided to check whether he still needed to synthesize more or not using a UV Vis spectrophotometer. Fortunately, he already prepared a 100.0 ppm AuNP standard solution beforehand. He got a different aliquot of the standard, added stabilizing solutions, and diluted them to 10.0 mL for the determination of absorbance. Data he obtained is shown below. Table 1. Calibration curve data for AuNP v of 100.0 ppm AuNP standard, mL Absorbance 0.00 0.200 0.50 0.450 1.00 0.510 1.50 0.600 2.00 0.680 3.00 0.810 4.00 0.950 After obtaining the calibration curve, he got a 3.00-mL aliquot of the pre-prepared solution, added stabilizing solution, and diluted it to 10 mL. He got 10mL of the final solution and determined its absorbance to be 0.650. The absorbance of the blank…a) Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) can be used as examples to explain difference between adsorption and partition. For each of these chromatographic techniques, identify: + the stationary and mobile phase + how the mobile phase moves b) How is the Rf value for a spot on a TLC plate calculated? What can the Rf value be used for? In order to achieve the best separation of several components of different polarity in a mixture spotted on a TLC plate, what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc.)
- The following data give the recovery of bromide from spiked samples of vegetable matter, measuredusing a gas–liquid chromatographic method. The same amount of bromide was added to each specimen(Roughan, J.A., Roughan, P.A. and Wilkins, J.P.G., 1983, Analyst, 108: 742).Tomato: 777 790 759 790 770 758 764 ug/gCucumber: 782 773 778 765 789 797 782 ug/g(a) Test whether the recoveries from the two vegetables have variances which differ significantly.(b) Test whether the mean recovery rates differ significantly.2. a) What is a bonded phase in HPLC?b) Give an example of a bonded phase in reversed-phase liquid chromatography and normal phase liquid chromatography.c) Describe when you would choose to use a reversed-phase column or a normal-phase column for an analysis of a mixture.Which statement is true? Statement 1: Since tiny amounts of material are exposed on an open surface, the use of TLC is limited to relatively nonvolatile substances.Statement 2: To determine the identity of a specific compound in a mixture, the TLC behavior of the test sample and that of authentic standard compounds are compared on the same plate.