Alfred was tasked to synthesize at least 250.0 ppm of his novel gold nanoparticles (AuNP’s) to be able to perform experiments about the efficacy of AuNP for viral detection. He decided to check whether he still needed to synthesize more or not using a UV Vis spectrophotometer. Fortunately, he already prepared a 100.0 ppm AuNP standard solution beforehand. He got a different aliquot of the standard, added stabilizing solutions, and diluted them to 10.0 mL for the determination of absorbance. Data he obtained is shown below. Table 1. Calibration curve data for AuNP v of 100.0 ppm AuNP standard, mL Absorbance 0.00 0.200 0.50 0.450 1.00 0.510 1.50 0.600 2.00 0.680 3.00 0.810 4.00 0.950 After obtaining the calibration curve, he got a 3.00-mL aliquot of the pre-prepared solution, added stabilizing solution, and diluted it to 10 mL. He got 10mL of the final solution and determined its absorbance to be 0.650. The absorbance of the blank solution for the sample solution was 0.200. b. What is the concentration of the final and pre-prepared solutions of novel AuNP? Is the solution enough for him to use? Express your answers in 3 significant figures [AuNP]final solution = _____ [AuNP]pre-prepared solution = ____ The concentration is _____ for use. c. If 10.0 mL of the pre-prepared solution was used to plate a 0.00100g polymer mesh with gold, what would be the total weight of the polymer mesh after?
Alfred was tasked to synthesize at least 250.0 ppm of his novel gold nanoparticles (AuNP’s) to be able to perform experiments about the efficacy of AuNP for viral detection. He decided to check whether he still needed to synthesize more or not using a UV Vis spectrophotometer.
Fortunately, he already prepared a 100.0 ppm AuNP standard solution beforehand. He got a different aliquot of the standard, added stabilizing solutions, and diluted them to 10.0 mL for the determination of absorbance. Data he obtained is shown below.
Table 1. Calibration curve data for AuNP
|
v of 100.0 ppm AuNP standard, mL |
Absorbance |
|
0.00 |
0.200 |
|
0.50 |
0.450 |
|
1.00 |
0.510 |
|
1.50 |
0.600 |
|
2.00 |
0.680 |
|
3.00 |
0.810 |
|
4.00 |
0.950 |
After obtaining the calibration curve, he got a 3.00-mL aliquot of the pre-prepared solution, added stabilizing solution, and diluted it to 10 mL. He got 10mL of the final solution and determined its absorbance to be 0.650. The absorbance of the blank solution for the sample solution was 0.200.
[AuNP]final solution = _____
[AuNP]pre-prepared solution = ____
The concentration is _____ for use.
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