4. Calculate the resolution from the chromatogram and table below for:a. peak #2 and peak #3b. peak #2 and peak #5Time Type0.873 B81.299 VVR1.444 VB2.141 685.31 68Height2.4229.8135.19.624Width0.04490.04890.05380.0640.1196Area6.7725.9461.639.6194.42.55891Example chromatogram: Y-axis = detector signal, X-axis = time.Peak identifiers: #2 = acetone (tm), #3 = acetaminophen, #4 = caffeine, #5 = aspirin.Mobile Phase (28% MeOH, 69% H2O, 3% GAA). 79% reservoir A, 21%B reservoir.BLES4)2.

To calculate resolution from the given chromatogram: a. peak #2 and peak #3 b. peak #2 and peak #5

Answered: 4. Calculate the resolution from the…

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4. Calculate the resolution from the chromatogram and table below for: a. peak #2 and peak # 3 b. peak #2 and peak #5

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter33: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 33.12QAP

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Question

4. Calculate the resolution from the chromatogram and table below for:
a. peak #2 and peak #3
b. peak #2 and peak #5
Time Type
0.873 B8
1.299 VVR
1.444 VB
2.141 68
5.31 68
Height
2.4
229.8
135.1
9.6
24
Width
0.0449
0.0489
0.0538
0.064
0.1196
Area
6.7
725.9
461.6
39.6
194.4
2.
5
589
1
Example chromatogram: Y-axis = detector signal, X-axis = time.
Peak identifiers: #2 = acetone (tm), #3 = acetaminophen, #4 = caffeine, #5 = aspirin.
Mobile Phase (28% MeOH, 69% H2O, 3% GAA). 79% reservoir A, 21%B reservoir.
BLES
4)
2.

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