The HPLC chromatogram below was collected using a reverse-phase column with a length of 10 cm. Estimate peak widths and heights from the graph to answer the questions below. 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Time (min) a. Calculate the resolution of the peaks. b. Calculate the number of theoretical plates in the column for analyte B. c. Calculate the theoretical plate height for analyte B. Signal

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter31: Introduction To Analytical Separations
Section: Chapter Questions
Problem 31.28QAP
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The HPLC chromatogram below was collected using a reverse-phase column with a length of 10 cm. Estimate
peak widths and heights from the graph to answer the questions below.
B
A
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Time (min)
a. Calculate the resolution of the peaks.
b. Calculate the number of theoretical plates in the column for analyte B.
c. Calculate the theoretical plate height for analyte B.
Signal
Transcribed Image Text:The HPLC chromatogram below was collected using a reverse-phase column with a length of 10 cm. Estimate peak widths and heights from the graph to answer the questions below. B A 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Time (min) a. Calculate the resolution of the peaks. b. Calculate the number of theoretical plates in the column for analyte B. c. Calculate the theoretical plate height for analyte B. Signal
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