5 Any Cu* ions present in solution will react with FerroZine in the same way as Fe2+. a. What effect would the presence of Cu²* in a patient's blood sample have on the determination of serum iron concentration? Explain. - Neocuproine is a compound that reacts selectively with Cu²+ and may be used to remove these ions from a patient sample, thereby preventing any interference with the determination of serum iron. Should the neocuproine be added to the patient sample before or after FerroZine? Explain your answer.

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter7: Statistical Data Treatment And Evaluation
Section: Chapter Questions
Problem 7.20QAP
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a. What effect would the presence of Cu2+ in a patient's blood sample have on the determination of serum iron
concentration? Explain.
5 Any Cu+ jons present in solution will react with FerroZine in the same way as Fe2+.
b. Neocuproine is a compound that reacts selectively with Cu2+ and may be used to remove these ions from a patient
sample, thereby preventing any interference with the determination of serum iron. Should the neocuproine be added
to the patient sample before or after FerroZine? Explain your answer.
6 The concentration of inorganic phosphate (PO,3-), another important ion in the body, may be determined by
spectrophotometric analysis. The phosphate is reacted with a compound to produce a deep-blue species that strongly
absorbs light at 650 nm. For an adult, normal phosphate levels are 2.4-4.4 mg/dL. Use the following data to prepare
a calibration curve, and then determine the concentration of phosphate for a patient whose sample gave an absorbance
value of 0.782. Is this patient's level within the normal range? Include a plot of your computer generated calibration
curve and show your work. Assume all samples were analyzed in a 1.0 cm pathlength cuvette.
PO,3- Concentration (mg/dL)
Absorbance at 562 nm
1.7
0.295
2.2
0.380
3.1
0.534
4.0
0.688
5.5
0.945
Transcribed Image Text:a. What effect would the presence of Cu2+ in a patient's blood sample have on the determination of serum iron concentration? Explain. 5 Any Cu+ jons present in solution will react with FerroZine in the same way as Fe2+. b. Neocuproine is a compound that reacts selectively with Cu2+ and may be used to remove these ions from a patient sample, thereby preventing any interference with the determination of serum iron. Should the neocuproine be added to the patient sample before or after FerroZine? Explain your answer. 6 The concentration of inorganic phosphate (PO,3-), another important ion in the body, may be determined by spectrophotometric analysis. The phosphate is reacted with a compound to produce a deep-blue species that strongly absorbs light at 650 nm. For an adult, normal phosphate levels are 2.4-4.4 mg/dL. Use the following data to prepare a calibration curve, and then determine the concentration of phosphate for a patient whose sample gave an absorbance value of 0.782. Is this patient's level within the normal range? Include a plot of your computer generated calibration curve and show your work. Assume all samples were analyzed in a 1.0 cm pathlength cuvette. PO,3- Concentration (mg/dL) Absorbance at 562 nm 1.7 0.295 2.2 0.380 3.1 0.534 4.0 0.688 5.5 0.945
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