5. By using Excel or GoogleSheets, graph the Lineweaver-Burk plots for the behavior of an enzyme for which the following experimental data are available. What are the Km and Vmax values for the inhibited and uninhibited reactions? Is the inhibitor competitive or noncompetitive? [S] (mM) V, No Inhibitor (mmol min-1) V, Inhibitor Present (mmol min-1) 1x 10-4 5 × 10-4 1.5 × 10-3 2.5 × 10-3 5 × 10-3 0.026 0.010 0.092 0.136 0.040 0.086 0.120 0.150 0.165 0.142

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter6: Energy, Enzymes, And Biological Reactions
Section: Chapter Questions
Problem 9TYK: Which of the following statements about inhibition is true? a. Allosteric inhibitors and allosteric...
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5. By using Excel or GoogleSheets. graph the Lineweaver-Burk plots for the behavior of
an enzyme for which the following experimental data are available. What are the Km and
Kwax values for the inhibited and uninhibited reactions? Is the inhibitor competitive or
noncompetitive?
[S]
(mM)
V, No Inhibitor
(mmol min-)
V, Inhibitor Present
(mmol min-')
1 × 10-4
5 × 10-4
1.5 x 10-3
2.5 x 10-3
5 x 10-3
0.026
0.010
0.092
0.136
0.040
0.086
0.150
0.120
0.165
0.142
Transcribed Image Text:5. By using Excel or GoogleSheets. graph the Lineweaver-Burk plots for the behavior of an enzyme for which the following experimental data are available. What are the Km and Kwax values for the inhibited and uninhibited reactions? Is the inhibitor competitive or noncompetitive? [S] (mM) V, No Inhibitor (mmol min-) V, Inhibitor Present (mmol min-') 1 × 10-4 5 × 10-4 1.5 x 10-3 2.5 x 10-3 5 x 10-3 0.026 0.010 0.092 0.136 0.040 0.086 0.150 0.120 0.165 0.142
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