A 7 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 31 colonies of bacteria grow on the petri dish.  Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using standard notation. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.

Biology (MindTap Course List)
11th Edition
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Chapter53: Introduction To Ecology: Population Ecology
Section53.2: Changes In Population Size
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A 7 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 31 colonies of bacteria grow on the petri dish. 

Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using standard notation.

  • Since only 0.1 mL is put on the plate, this counts as an extra dilution!!!
  • Any time less than 1 mL is transfered, a dilution is being performed.
  • Any time more than 1 mL is transfered, a concentration is being performed.
 
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